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Thomas Lehnert

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Related publications (52)

Automated longitudinal monitoring of in vivo protein aggregation in neurodegenerative disease C. elegans models

Thomas Lehnert, Martinus Gijs, Johan Auwerx, Matteo Cornaglia, Laurent Mouchiroud, Govindan Narayanan Krishnamani, Vincenzo Sorrentino

Background: While many biological studies can be performed on cell-based systems, the investigation of molecular pathways related to complex human dysfunctions - e.g. neurodegenerative diseases - often requires long-term studies in animal models. The nemat ...

BioMed Central2016

On-chip microfluidic biocommunication assay for studying male-induced demise in C. elegans hermaphrodites

Thomas Lehnert, Martinus Gijs, Matteo Cornaglia, Li Dong

Like other animals, C. elegans nematodes have the ability to socially interact and to communicate through exchange and sensing of small soluble signaling compounds that help them cope with complex environmental conditions. For the time being, worm biocommu ...

Royal Soc Chemistry2016

Size-dependent sorting of C. elegans nematodes through an adjustable microfluidic filter structure

Thomas Lehnert, Martinus Gijs, Matteo Cornaglia, Li Dong

We introduce a new and simple microfluidic sorting device based on adjustable filter structures that can be easily tuned by external pressure for a specific size-dependent C. elegans worm sorting experiment. A single device can be used for selecting and so ...

2016

Versatile size-dependent sorting of C-elegans nematodes and embryos using a tunable microfluidic filter structure

Thomas Lehnert, Martinus Gijs, Matteo Cornaglia, Li Dong

The roundworm Caenorhabditis elegans (C. elegans) is a powerful model organism for addressing fundamental biological questions related to human disease and aging. Its life cycle consists of an embryo stage, four larval stages that can be clearly distinguis ...

Royal Soc Chemistry2016

An automated microfluidic platform for C. elegans embryo arraying, phenotyping, and long-term live imaging

Thomas Lehnert, Martinus Gijs, Johan Auwerx, Shreya Narasimhan, Matteo Cornaglia, Laurent Mouchiroud, Alexis Pierre Henri Marette, Virginija Jovaisaite

Studies of the real-time dynamics of embryonic development require a gentle embryo handling method, the possibility of long-term live imaging during the complete embryogenesis, as well as of parallelization providing a population's statistics, while keepin ...

Nature Publishing Group2015

Dose-response curve of a microfluidic magnetic bead-based surface coverage sandwich assay

Thomas Lehnert, Martinus Gijs, Hüseyin Cumhur Tekin, Matteo Cornaglia, Raphaël Etienne Jean Trouillon

Magnetic micro-and nanoparticles ('magnetic beads') have been used to advantage in many microfluidic devices for sensitive antigen (Ag) detection. Today, assays that use as read-out of the signal the number count of immobilized beads on a surface for quant ...

Elsevier2015

Nanocalorimetric platform for accurate thermochemical studies in microliter volumes

Thomas Lehnert, Martinus Gijs, Rima Padovani, Raphaël Etienne Jean Trouillon

A straightforward and general way for monitoring chemical reactions is via their thermal signature. Such approach requires however an experimental setup with a high thermal stability that simultaneously allows time-resolved heat detection with high sensiti ...

Royal Soc Chemistry2015

Study of constrained Brownian motion of nanoparticles near an interface using optical tweezers

Thomas Lehnert, Martinus Gijs, Hui Yang, Matteo Cornaglia, Raphaël Etienne Jean Trouillon

We demonstrate a method to determine the Brownian motion and the diffusion coefficient of a nanoparticle in water in a plane that is parallel to a solid boundary and as function of the distance normal to that boundary by using an optical tweezers instrumen ...

Spie-Int Soc Optical Engineering2015

A random-walk based model to explain ultrasensitive magnetic bead-based immunoassays

Thomas Lehnert, Martinus Gijs, Hüseyin Cumhur Tekin, Matteo Cornaglia, Raphaël Etienne Jean Trouillon

We introduce a new modeling approach for understanding the fundamental principle of bead-based surface coverage immunoassays that rely on the specific interaction between surface-bound “small” magnetic beads (1 μm in diameter) and a flow of “large” magneti ...

2014

Magnetic Particle-Scanning for Ultrasensitive Immunodetection On-Chip

Thomas Lehnert, Martinus Gijs, Hüseyin Cumhur Tekin, Matteo Cornaglia, Raphaël Etienne Jean Trouillon

We describe the concept of magnetic particle-scanning for on-chip detection of biomolecules: a magnetic particle, carrying a low number of antigens (Ag's) (down to a single molecule), is transported by hydrodynamic forces and is subjected to successive sto ...

American Chemical Society2014

An automated microfluidic platform for reversible immobilization and imaging of C. elegans

Thomas Lehnert, Martinus Gijs, Johan Auwerx, Matteo Cornaglia, Marie-Pierre Meurville, Laurent Mouchiroud, Govindan Narayanan Krishnamani

2014

An automated microfluidic platform for long-term high-resolution imaging of C. elegans

Thomas Lehnert, Martinus Gijs, Johan Auwerx, Matteo Cornaglia, Marie-Pierre Meurville, Laurent Mouchiroud, Govindan Narayanan Krishnamani

We describe a microfluidic platform for the automated culture, treatment and long-term highresolution imaging of Caenorhabditis elegans nematodes under normal physiological conditions. Our device features: (i) a microfluidic design tailored for the isolati ...

2014

Fine-tuning of magnetic and microfluidic viscous forces for specific magnetic bead-based immunocomplex formation

Thomas Lehnert, Martinus Gijs, Hüseyin Cumhur Tekin, Matteo Cornaglia

We investigate the working principle of a novel type of microfluidic sandwich immunoassay, as used for the detection of biomarkers. The heterogeneous assay is based on the specific interactions between an array of functionalized superparamagnetic beads and ...

American Institute of Physics2013

Surface Microstructures on Planar Substrates and Textile Fibers Guide Neurite Outgrowth: A Scaffold Solution to Push Limits of Critical Nerve Defect Regeneration?

Thomas Lehnert, Li Yao

The treatment of critical size peripheral nerve defects represents one of the most serious problems in neurosurgery. If the gap size exceeds a certain limit, healing can't be achieved. Connection mismatching may further reduce the clinical success. The pre ...

Public Library Science2012

Magnetic particle dosing and size separation in a microfluidic channel

Thomas Lehnert, Martinus Gijs, Yves Moser, Rana Afshar Ghasemlouy

Separation of functional magnetic particles or magnetically labeled entities is a key feature for bioanalytical or biomedical applications and therefore also an important component of lab-on-a-chip devices for biological applications. We present a novel in ...

2011

Suspended SU-8 structures for monolithic microfluidic channels

Thomas Lehnert, Martinus Gijs, Sébastien Jiguet, Yves Moser, Riccardo Forti

SU-8 photoresist is commonly used in the field of microfabrication as structural material or for molding of microfluidic devices. One major limitation, however, is the difficulty to process partially freestanding SU-8 structures or monolithic closed caviti ...

Springer-Verlag2011

Three-Dimensional Magnetic Focusing of Superparamagnetic Beads for On-Chip Agglutination Assays

Thomas Lehnert, Martinus Gijs, Yves Moser, Rana Afshar Ghasemlouy

We present a new method for three-dimensional (3D) magnetic focusing of magnetic microparticles in a microfluidic system. On-chip magnetic particle manipulation in themicrochannel is achieved by a high-gradientmagnetic field generated by means of a microma ...

2011

Magneto-microfluidic three-dimensional focusing of magnetic particles

Thomas Lehnert, Martinus Gijs, Yves Moser, Rana Afshar Ghasemlouy

We present a method for magneto-microfluidic three-dimensional focusing of magnetic microparticles from a dense plug into a single streamline with longitudinal inter-particle spacing. Plug formation on-chip is induced by a high-gradient magnetic field gene ...

Amer Inst Physics, 2 Huntington Quadrangle, Ste 1No1 D, Melville, Ny 11747-4501 Usa2010

A quadrupolar magnetic actuation system for superparamagnetic particles

Thomas Lehnert, Martinus Gijs, Yves Moser

2009

Quadrupolar magnetic actuation of superparamagnetic particles for enhanced microfluidic perfusion

Thomas Lehnert, Martinus Gijs, Yves Moser

We report on the magnetic actuation of superparamagnetic particles in a microfluidic channel. The formation of a highly confined and dynamic plug of particles extending over the cross section of the microchannel is enabled by superposing a static and a tim ...

AIP American Institute of Physics2009

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