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Thomas Lehnert

Cette personne a quitté l’EPFL

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Publications associées (49)

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The enhancement of DNA fragmentation in a bench top ultrasonic water bath with needle-induced air bubbles: Simulation and experimental investigation

Martinus Adela Maria Gijs, Thomas Lehnert

Shearing DNA to a certain size is the first step in many medical and biological applications, especially in next-generation gene sequencing technology. In this article, we introduced a highly efficient ultrasonic DNA fragmentation method enhanced by needle ...

AIP Publishing2022

Magnetic Particle-Scanning for Ultrasensitive Immunodetection On-Chip

Martinus Adela Maria Gijs, Thomas Lehnert, Matteo Cornaglia, Hüseyin Cumhur Tekin, Raphaël Etienne Jean Trouillon

We describe the concept of magnetic particle-scanning for on-chip detection of biomolecules: a magnetic particle, carrying a low number of antigens (Ag's) (down to a single molecule), is transported by hydrodynamic forces and is subjected to successive sto ...

American Chemical Society2014

An automated microfluidic platform for long-term high-resolution imaging of C. elegans

Martinus Adela Maria Gijs, Johan Auwerx, Thomas Lehnert, Matteo Cornaglia, Laurent Mouchiroud, Govindan Narayanan Krishnamani, Marie-Pierre Meurville, Gopalan Hariharan Krishnamani

We describe a microfluidic platform for the automated culture, treatment and long-term highresolution imaging of Caenorhabditis elegans nematodes under normal physiological conditions. Our device features: (i) a microfluidic design tailored for the isolati ...

2014

Automated longitudinal monitoring of in vivo protein aggregation in neurodegenerative disease C. elegans models

Martinus Adela Maria Gijs, Johan Auwerx, Thomas Lehnert, Matteo Cornaglia, Laurent Mouchiroud, Vincenzo Sorrentino, Govindan Narayanan Krishnamani

Background: While many biological studies can be performed on cell-based systems, the investigation of molecular pathways related to complex human dysfunctions - e.g. neurodegenerative diseases - often requires long-term studies in animal models. The nemat ...

BioMed Central2016

Polydimethylsiloxane microstructure-induced acoustic streaming for enhanced ultrasonic DNA fragmentation on a microfluidic chip

Martinus Adela Maria Gijs, Thomas Lehnert

Next-generation sequencing (NGS) is an essential technology for DNA identification in genomic research. DNA fragmentation is a critical step for NGS and doing this on-chip is of great interest for future integrated genomic solutions. Here we demonstrate fa ...

ROYAL SOC CHEMISTRY2022

Microfluidic system for Caenorhabditis elegans culture and oxygen consumption rate measurements

Martinus Adela Maria Gijs, Thomas Lehnert, Matteo Cornaglia, Roger Philippe Krenger

Mitochondrial respiration is a key signature for the assessment of mitochondrial functioning and mitochondrial dysfunction is related to many diseases including metabolic syndrome and aging-associated conditions. Here, we present a microfluidic Caenorhabdi ...

ROYAL SOC CHEMISTRY2020

Magneto-microfluidic three-dimensional focusing of magnetic particles

Martinus Adela Maria Gijs, Thomas Lehnert, Rana Afshar Ghasemlouy

We present a method for magneto-microfluidic three-dimensional focusing of magnetic microparticles from a dense plug into a single streamline with longitudinal inter-particle spacing. Plug formation on-chip is induced by a high-gradient magnetic field gene ...

Amer Inst Physics, 2 Huntington Quadrangle, Ste 1No1 D, Melville, Ny 11747-4501 Usa2010

A quadrupolar magnetic actuation system for superparamagnetic particles

Martinus Adela Maria Gijs, Thomas Lehnert

2009

8-Channel Single Embryo Pipette For Accurate C-Elegans Bioassays

Martinus Adela Maria Gijs, Thomas Lehnert, Li Dong

The nematode Caenorhabditis elegans (C. elegans) is a powerful model organism for addressing fundamental biological questions related to human disease and aging. Accurate control of the number of worms is a primary step for quantitative biological or bioch ...

IEEE2018

Track C. Biomedical Instrumentation & Micro and Nano Systems

Johan Auwerx, Martinus Adela Maria Gijs, Thomas Lehnert, Laurent Mouchiroud, Matteo Cornaglia, Vincenzo Sorrentino, Alexis Pierre Henri Marette, Virginija Jovaisaite, Govindan Narayanan Krishnamani

De Gruyter2016

Size-dependent sorting of C. elegans nematodes through an adjustable microfluidic filter structure

Martinus Adela Maria Gijs, Thomas Lehnert, Matteo Cornaglia, Li Dong

We introduce a new and simple microfluidic sorting device based on adjustable filter structures that can be easily tuned by external pressure for a specific size-dependent C. elegans worm sorting experiment. A single device can be used for selecting and so ...

2016

Bubble-enhanced ultrasonic microfluidic chip for rapid DNA fragmentation (vol 22, pg 560, 2022)

Martinus Adela Maria Gijs, Thomas Lehnert

ROYAL SOC CHEMISTRY2023

Magnetic particle dosing and size separation in a microfluidic channel

Martinus Adela Maria Gijs, Thomas Lehnert, Rana Afshar Ghasemlouy

Separation of functional magnetic particles or magnetically labeled entities is a key feature for bioanalytical or biomedical applications and therefore also an important component of lab-on-a-chip devices for biological applications. We present a novel in ...

2011

Fine-tuning of magnetic and microfluidic viscous forces for specific magnetic bead-based immunocomplex formation

Martinus Adela Maria Gijs, Thomas Lehnert, Matteo Cornaglia, Hüseyin Cumhur Tekin

We investigate the working principle of a novel type of microfluidic sandwich immunoassay, as used for the detection of biomarkers. The heterogeneous assay is based on the specific interactions between an array of functionalized superparamagnetic beads and ...

American Institute of Physics2013

Magnetic particle dosing, release and separation in a microfluidic chip with magnetic actuation

Martinus Adela Maria Gijs, Thomas Lehnert, Rana Afshar Ghasemlouy

2009

An automated microfluidic platform for reversible immobilization and imaging of C. elegans

Martinus Adela Maria Gijs, Johan Auwerx, Thomas Lehnert, Matteo Cornaglia, Laurent Mouchiroud, Govindan Narayanan Krishnamani, Marie-Pierre Meurville, Gopalan Hariharan Krishnamani

2014

Microfluidic integration of gel-based reference electrodes for electrophysiological measurements on chip

Martinus Adela Maria Gijs, Thomas Lehnert

2006

Suspended SU-8 structures for monolithic microfluidic channels

Martinus Adela Maria Gijs, Thomas Lehnert, Sébastien Jiguet

SU-8 photoresist is commonly used in the field of microfabrication as structural material or for molding of microfluidic devices. One major limitation, however, is the difficulty to process partially freestanding SU-8 structures or monolithic closed caviti ...

Springer-Verlag2011

Microfluidic chip for the separation of magnetic particles

Martinus Adela Maria Gijs, Thomas Lehnert, Rana Afshar Ghasemlouy

2007

Defect structure in micropillars using X-ray microdiffraction

Martinus Adela Maria Gijs, Helena Van Swygenhoven, Thomas Lehnert, Steven Van Petegem

AIP American Institute of Physics2006