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Thomas Lehnert

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Related publications (49)

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Microfluidic systems for high-throughput and high-content screening using the nematode Caenorhabditis elegans

Martinus Adela Maria Gijs, Thomas Lehnert, Matteo Cornaglia

In a typical high-throughput drug screening (HTS) process, up to millions of chemical compounds are applied to cells cultured in well plates, aiming to find molecules that exhibit a robust dose-response, as evidenced for example by a fluorescence signal. I ...

Royal Soc Chemistry2017

Versatile size-dependent sorting of C-elegans nematodes and embryos using a tunable microfluidic filter structure

Martinus Adela Maria Gijs, Thomas Lehnert, Matteo Cornaglia, Li Dong

The roundworm Caenorhabditis elegans (C. elegans) is a powerful model organism for addressing fundamental biological questions related to human disease and aging. Its life cycle consists of an embryo stage, four larval stages that can be clearly distinguis ...

Royal Soc Chemistry2016

Microfluidic systems for infectious disease diagnostics

Martinus Adela Maria Gijs, Thomas Lehnert

Microorganisms, encompassing both uni- and multicellular entities, exhibit remarkable diversity as omnipresent life forms in nature. They play a pivotal role by supplying essential components for sustaining biological processes across diverse ecosystems, i ...

Royal Soc Chemistry2024

Effect of inoculum size and antibiotics on bacterial traveling bands in a thin microchannel defined by optical adhesive

Martinus Adela Maria Gijs, Thomas Lehnert

Phenotypic diversity in bacterial flagella-induced motility leads to complex collective swimming patterns, appearing as traveling bands with transient locally enhanced cell densities. Traveling bands are known to be a bacterial chemotactic response to self ...

SPRINGERNATURE2021

Bubble-enhanced ultrasonic microfluidic chip for rapid DNA fragmentation

Martinus Adela Maria Gijs, Thomas Lehnert

DNA fragmentation is an essential process in developing genetic sequencing strategies, genetic research, as well as for the diagnosis of diseases with a genetic signature like cancer. Efficient on-chip DNA fragmentation protocols would be beneficial to pro ...

ROYAL SOC CHEMISTRY2022

On-chip microfluidic biocommunication assay for studying male-induced demise in C. elegans hermaphrodites

Martinus Adela Maria Gijs, Thomas Lehnert, Matteo Cornaglia, Li Dong

Like other animals, C. elegans nematodes have the ability to socially interact and to communicate through exchange and sensing of small soluble signaling compounds that help them cope with complex environmental conditions. For the time being, worm biocommu ...

Royal Soc Chemistry2016

Dose-response curve of a microfluidic magnetic bead-based surface coverage sandwich assay

Martinus Adela Maria Gijs, Thomas Lehnert, Matteo Cornaglia, Hüseyin Cumhur Tekin, Raphaël Etienne Jean Trouillon

Magnetic micro-and nanoparticles ('magnetic beads') have been used to advantage in many microfluidic devices for sensitive antigen (Ag) detection. Today, assays that use as read-out of the signal the number count of immobilized beads on a surface for quant ...

Elsevier2015

Surface Microstructures on Planar Substrates and Textile Fibers Guide Neurite Outgrowth: A Scaffold Solution to Push Limits of Critical Nerve Defect Regeneration?

Thomas Lehnert

The treatment of critical size peripheral nerve defects represents one of the most serious problems in neurosurgery. If the gap size exceeds a certain limit, healing can't be achieved. Connection mismatching may further reduce the clinical success. The pre ...

Public Library Science2012

Three-Dimensional Magnetic Focusing of Superparamagnetic Beads for On-Chip Agglutination Assays

Martinus Adela Maria Gijs, Thomas Lehnert, Rana Afshar Ghasemlouy

We present a new method for three-dimensional (3D) magnetic focusing of magnetic microparticles in a microfluidic system. On-chip magnetic particle manipulation in themicrochannel is achieved by a high-gradientmagnetic field generated by means of a microma ...

2011

Anin vivomicrofluidic study of bacterial transit inC. elegansnematodes

Martinus Adela Maria Gijs, Thomas Lehnert, Matteo Cornaglia, Hüseyin Baris Atakan, Vittorio Viri

Caenorhabditis elegans(C. elegans) constitutes an important model organism for use in nutrition and aging studies. We report a novel method for studying the dynamics ofEscherichia coli(E. coli) bacterial transit through the worms' intestine. A microfluidic ...

2020

C. Elegans Immobilization Using Deformable Microfluidics For In Vivo Studies Of Early Embryogenesis And Intestinal Microbiota

Martinus Adela Maria Gijs, Thomas Lehnert, Matteo Cornaglia, Li Dong, Xiaopeng Huang

The genome of the nematode Caenorhabditis elegans shows far-reaching similarities to that of humans. C. elegans is therefore considered as a powerful model organism in biological research related to humans, for instance for the study of human diseases. Suc ...

Ieee2017

Force microscopy of the Caenorhabditis elegans embryonic eggshell

Martinus Adela Maria Gijs, Thomas Lehnert, Roger Philippe Krenger

Assays focusing on emerging biological phenomena in an animal's life can be performed during embryogenesis. While the embryo of Caenorhabditis elegans has been extensively studied, its biomechanical properties are largely unknown. Here, we demonstrate that ...

NATURE PUBLISHING GROUP2020

Quadrupolar magnetic actuation of superparamagnetic particles for enhanced microfluidic perfusion

Martinus Adela Maria Gijs, Thomas Lehnert

We report on the magnetic actuation of superparamagnetic particles in a microfluidic channel. The formation of a highly confined and dynamic plug of particles extending over the cross section of the microchannel is enabled by superposing a static and a tim ...

AIP American Institute of Physics2009

An Integrated Microfluidic Device For C-Elegans Early Embryogenesis Studies And Drug Assays

Martinus Adela Maria Gijs, Pierre Gönczy, Thomas Lehnert, Matteo Cornaglia, Li Dong, Radek Jankele

The Caenorhabditis elegans embryo is a widely used model for the functional analysis of dynamic cellular processes, such as chromosome segregation, cytokinesis or lineage analysis. However, the conventional embryo preparation method that relies on manually ...

Ieee2017

An automated microfluidic platform for C. elegans embryo arraying, phenotyping, and long-term live imaging

Martinus Adela Maria Gijs, Johan Auwerx, Thomas Lehnert, Matteo Cornaglia, Laurent Mouchiroud, Alexis Pierre Henri Marette, Virginija Jovaisaite

Studies of the real-time dynamics of embryonic development require a gentle embryo handling method, the possibility of long-term live imaging during the complete embryogenesis, as well as of parallelization providing a population's statistics, while keepin ...

Nature Publishing Group2015

Nanocalorimetric platform for accurate thermochemical studies in microliter volumes

Martinus Adela Maria Gijs, Thomas Lehnert, Raphaël Etienne Jean Trouillon, Rima Padovani

A straightforward and general way for monitoring chemical reactions is via their thermal signature. Such approach requires however an experimental setup with a high thermal stability that simultaneously allows time-resolved heat detection with high sensiti ...

Royal Soc Chemistry2015

An In Vivo Microfluidic Study of Bacterial Load Dynamics and Absorption in the C. elegans Intestine

Martinus Adela Maria Gijs, Thomas Lehnert, Vittorio Viri

Caenorhabditiselegans (C. elegans) has gained importance as a model for studying host-microbiota interactions and bacterial infections related to human pathogens. Assessing the fate of ingested bacteria in the worm's intestine is therefore of great interes ...

MDPI2021

Antimicrobial susceptibility testing by measuring bacterial oxygen consumption on an integrated platform

Martinus Adela Maria Gijs, Thomas Lehnert

Cellular respiration is a fundamental feature of metabolic activity and oxygen consumption can be considered as a reliable indicator of bacterial aerobic respiration, including for facultative anaerobic bacteria like E. coli. Addressing the emerging global ...

ROYAL SOC CHEMISTRY2021

Fast antimicrobial susceptibility testing onEscherichia coliby metabolic heat nanocalorimetry

Martinus Adela Maria Gijs, Thomas Lehnert, Yang Liu

Fast spreading of antimicrobial resistance is now considered a major global health threat. New technologies are required, enabling rapid diagnostics of bacterial infection combined with fast antimicrobial susceptibility testing (AST) for evaluating the eff ...

2020

Study of constrained Brownian motion of nanoparticles near an interface using optical tweezers

Martinus Adela Maria Gijs, Thomas Lehnert, Matteo Cornaglia, Raphaël Etienne Jean Trouillon, Hui Yang

We demonstrate a method to determine the Brownian motion and the diffusion coefficient of a nanoparticle in water in a plane that is parallel to a solid boundary and as function of the distance normal to that boundary by using an optical tweezers instrumen ...

Spie-Int Soc Optical Engineering2015