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Horst Arthur Vogel

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Related publications (115)

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Visualizing odorant receptor trafficking in living cells down to the single-molecule level

Horst Arthur Vogel, Horst Pick, Jean-Manuel Segura, Valérie Jacquier

Despite the importance of trafficking for regulating G protein-coupled receptor signaling, for many members of the seven transmembrane helix protein family, such as odorant receptors, little is known about this process in live cells. Here, the complete lif ...

National Academy of Sciences2006

Microfluidic array cytometer based on refractive optical tweezers for parallel trapping, imaging and sorting of individual cells

René Salathé, Horst Arthur Vogel, Fabrice Merenda, Joachim Piguet, Michael Werner

Analysis of genetic and functional variability in populations of living cells requires experimental techniques capable of monitoring cellular processes such as cell signaling of many single cells in parallel while offering the possibility to sort interesti ...

Royal Society of Chemistry2011

Two-dimensional crystallization of the mouse serotonin 5-HT3A receptor

Horst Arthur Vogel, Henning Paul-Julius Stahlberg, Ghérici Hassaïne

The mouse serotonin 5-HT3A receptor is a homo-pentameric ligand-gated ion channel (pLGIC) mediating fast excitatory neurotransmission in the central nervous system. The molecular mechanism of ion permeation of 5-HT3A receptors triggered by the neurotransmi ...

Pergamon-Elsevier Science Ltd2017

Supported cell-membrane sheets for functional fluorescence imaging of membrane proteins

Horst Arthur Vogel, Jean-Manuel Segura, Jean-Baptiste Perez

Cell-membrane sheets suitable for in-vitro functional fluorescence studies have been prepd. by direct detachment from cell membranes using poly-L-lysine-coated glass slides. The resulting transferred planar membranes conserve the compn. as well as most pro ...

2006

A synthetic membrane protein in tethered lipid bilayers for immunosensing in whole blood

Horst Arthur Vogel, Samuel Terrettaz, Solomzi Makohliso

Tethered lipid bilayers, containing a transmembrane synthetic ligand-gated ion channel (SLIC), have been formed on gold surfaces. The SLIC was designed as a highly selective receptor and reporter protein to detect antibodies in whole blood, which are of im ...

Elsevier2009

Single-Vesicle Assays Using Liposomes and Cell-Derived Vesicles: From Modeling Complex Membrane Processes to Synthetic Biology and Biomedical Applications

Ana Catarina Rodrigues Alves, Horst Arthur Vogel, Horst Pick

The plasma membrane is of central importance for defining the closed volume of cells in contradistinction to the extracellular environment. The plasma membrane not only serves as a boundary, but it also mediates the exchange of physical and chemical inform ...

AMER CHEMICAL SOC2018

Diffusion-Time Distribution Analysis Reveals Characteristic Ligand-Dependent Interaction Patterns of Nuclear Receptors in Living Cells

Horst Arthur Vogel, Horst Pick, Hanna Jankevics, Paulina Izewska

Nuclear receptors initiate transcription, interact with regulatory proteins, and are influenced by hormones, drugs, and pollutants. Herein, we discover ligand-specific mobility patterns of human estrogen receptor-a (ER) in living cells using diffusion-time ...

2005

Deamidation and Transamidation of Substance P by Tissue Transglutaminase Revealed by Electron-Capture Dissociation Fourier Transform Mass Spectrometry (Chem. Eur. J. 2/2011)

Horst Arthur Vogel, Yury Tsybin, Adrian Schmid, Luca Fornelli, Luigino Grasso

Tissue transglutaminase (tTGase) catalyzes both deamidation and transamidation of peptides and proteins by using a peptidyl glutamine as primary substrate. A precise consensus sequence for the enzyme is unknown and the ratio between deamidated and transami ...

Wiley-Blackwell2011

The Structure of the Mouse Serotonin 5-HT3 Receptor in Lipid Vesicles

Horst Arthur Vogel, Henning Paul-Julius Stahlberg, Cédric Deluz, Luigino Grasso, Ghérici Hassaïne

The function of membrane proteins is best understood if their structure in the lipid membrane is known. Here, we determined the structure of the mouse serotonin 5-HT3 receptor inserted in lipid bilayers to a resolution of 12 Å without stabilizing antibodie ...

Elsevier2016

The Mouse Eugenol Odorant Receptor: Structural and Functional Plasticity of a Broadly Tuned Odorant Binding Pocket

Horst Arthur Vogel, Horst Pick, Olivia Baud, Sylvain Etter

Molecular interactions of odorants with their olfactory receptors (ORs) are of central importance for the ability of the mammalian olfactory system to detect and discriminate a vast variety of odors with a limited set of receptors. How a particular OR bind ...

American Chemical Society2011

Kinetics of the initial steps of G protein-coupled receptor-mediated cellular signaling revealed by single-molecule imaging

Horst Arthur Vogel, Bruno Meyer

We report on an in vivo single-mol. study of the signaling kinetics of G protein-coupled receptors (GPCR) performed using the neurokinin 1 receptor (NK1R) as a representative member. The NK1R signaling cascade is triggered by the specific binding of a fluo ...

2005

Ligand binding transmits conformational changes across the membrane-spanning region to the intracellular side of the 5-HT3 serotonin receptor

Horst Arthur Vogel, Horst Pick, Cédric Deluz, Erwin Ilegems

Conformational changes of channel activation: Five enhanced green fluorescent protein (EGFP) mols. (green cylinders) were integrated into the intracellular part of the homopentameric ionotropic 5-HT3 receptor. This allowed the detection of extracellular bi ...

2005

Study of the solar anisotropy of cosmic ray primaries of about 200 GeV energy with the L3+C muon detector

Qian Wang, Zhirui Xu, Aurelio Bay, Liang Sun, Horst Arthur Vogel, Jian Zhao, Eduardo Sanchez

Context. Primary cosmic rays experience multiple deflections in the non-uniform galactic and heliospheric magnetic fields which may generate anisotropies. Aims. A study of anisotropies in the energy range between 100 and 500 GeV is performed. This energy r ...

2008

Mechanistic Studies on the Stereoselectivity of the Serotonin 5-HT1A Receptor

Horst Arthur Vogel, Shuguang Yuan

G-protein-coupled receptors (GPCRs) are involved in a wide range of physiological processes, and they have attracted considerable attention as important targets for developing new medicines. A central and largely unresolved question in drug discovery, whic ...

Wiley-Blackwell2016

Repetitive Reversible Labeling of Proteins at Polyhistidine Sequences for Single-Molecule Imaging in Live Cells

Horst Arthur Vogel, Rudolf Hovius, Jean-Manuel Segura, Emmanuel Guignet

Sensitive live-cell fluorescence microscopy and single-molecule imaging are severely limited by rapid photobleaching of fluorescent probes. Herein, we show how to circumvent this problem using a novel, generic labeling strategy. Small nickel-nitrilotriacet ...

2007

The Molecular Mechanism of P2Y 1 Receptor Activation

Horst Arthur Vogel, Shuguang Yuan

Human purinergic Gprotein-coupled receptor P2Y(1) (P2Y(1)R) is activated by adenosine 5'-diphosphate (ADP) to induce platelet activation and thereby serves as an important antithrombotic drug target. Crystal structures of P2Y(1)R revealed that one ligand ( ...

Wiley-Blackwell2016

Refractive multiple optical tweezers for parallel biochemical analysis in micro-fluidics

René Salathé, Horst Arthur Vogel, Fabrice Merenda, Johann Rohner

We present a multiple laser tweezers system based on refractive optics. The system produces an array of 100 optical traps thanks to a refractive microlens array, whose focal plane is imaged into the focal plane of a high-NA microscope objective. This refra ...

2007

New conjugate of agonist ligand directed to ligand-gated ion channels of cysteine loop-family with chromophore, useful, e.g. in receptor proteins/their interaction with agonistic ligand and to study the effect of unlabelled ligands

Horst Arthur Vogel, Rudolf Hovius, Jörg Grandl

NOVELTY - Conjugate (I) of an agonist ligand directed to ligand-gated ion channels (LGICs) of the Cys loop-family with a chromophore, is new. USE - (I) is useful in the receptor proteins and/or their interaction with the agonistic ligand, where the interac ...

2007

Enhancing Multifunctional Drug Screening via Artificial Intelligence

Horst Arthur Vogel

Computational drug screening is of fundamental importance in early-stage drug discovery. The current computational methods predict compound affinities to their targets based on docking or pharmacophore (PH4) hypotheses. Here, we develop Alpha-Pharm3D, a ve ...

ROYAL SOC CHEMISTRY2025

Functional asymmetry of transmembrane segments in nicotinic acetylcholine receptors

Horst Arthur Vogel, Rudolf Hovius, Christophe Danelon, Jörg Grandl

Nicotinic acetylcholine receptors are heteropentameric ion channels that open upon activation to a single conducting state. The second transmembrane segments of each subunit were identified as channel-forming elements, but their respective contribution in ...

Springer-Verlag2006