Publications by Andrzej Kulik | EPFL Graph Search

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The diagnosis of muscular dystrophies or the assessment of the functional benefit of gene or cell therapies can be difficult, especially for poorly accessible muscles, and it often lacks a single-fiber resolution. In the present study, we evaluated whether muscle diseases can be diagnosed from small biopsies using atomic force microscopy (AFM). AFM was shown to provide a sensitive and quantitative description of the resistance of normal and dystrophic myofibers within live muscle tissues explanted from Duch

This study reports HIPIMS-sputtered samples of Cu-particulate films with currents at 6 and 60 amps leading to E. coil inactivation. The Cu coverage and nanoparticle structure of the fibers is reported by TEM. Evidence is presented of redox processes in the ...

Droplet microfluidics has emerged as a powerful platform allowing a large number of individual reactions to be carried out in spatially distinct microcompartments. Due to their small size, however, the spectroscopic characterisation of species encapsulated ...

We propose and demonstrate the ability and efficiency of using a universal file format for a nanolithography pattern. A problem faced by the physicists working in the field of nanolithography is a lack of a flexible pattern design software (possibly open–source) that could be applied in combination with a broad range of commercial scanning probe microscope (SPM) systems. The current nanolithography software packages are device–specific and not portable. Therefore, it is impossible to make a lithography patt

Atomic force microscopy has been used to investigate the mechanical properties of microtubules. Method of Salvetat et al. [1], originally developed for measuring the Young's modulus of carbon nanotubes, has been extended to a biological system of similar geometry. Preliminary measurements of Young's modulus of microtubules in this configuration give a value of 74 MPa. Compression measurements on the same system give a value of 22 MPa, which is of the same order of magnitude, while values previously reported

Many proteins in alive organisms have a domain structure providing them the possibility to reversible unfolding, which seems to play an essential role in those processes occurring in tissues which are controlled by mechanical cellular tension. In this work ...

Aim: Atomic force microscopy nanoindentation of myofibers was used to assess and quantitatively diagnose muscular dystrophies from human patients. Materials & methods: Myofibers were probed from fresh or frozen muscle biopsies from human dystrophic patient ...

We propose a system to characterize the 3-D diffusion properties of the probing bead trapped by a photonic-force microscope. We follow a model-based approach, where the model of the dynamics of the bead is given by the Langevin equation. Our procedure comb ...

The integrity of the chromatin structure is essential to every process occurring within eukaryotic nuclei. However, there are no reliable tools to decipher the molecular composition of metaphase chromosomes. Here, we have applied infrared nanospectroscopy ...

Polyethylene block-copolymer films containing negative anhydride groups were used to immobilize TiO2, Fe2O3, and Fe3+ photocatalysts. The kinetics of the mineralization of azo-dye Orange II and chlorophenols on copolymer−TiO2, copolymer−Fe2O3, and copolymer−Fe3+ have been tested under optimized experimental conditions. In the case of copolymer−TiO2, the degradation kinetics for the model organic compounds were about the same as those observed with TiO2 suspensions containing about a 27 times higher amount o

The first experiment showing the effects of specific interaction forces using lateral force microscopy (LFM) was demonstrated for lectin-carbohydrate interactions some years ago. Such measurements are possible under the assumption that specific forces stro ...

Hydrogel microspheres, beads, and capsules of uniform size, differing in their chem. compn., have been prepd. by electrostatic complex formation of sodium alginate with divalent cations and polycations. These have served as model spheres to study the influence of the chem. compn. on both surface characteristics and bulk mech. properties. Resistance to compression expts. yielding the compression work clearly identified differences as a function of the compn., with forces at maximal compression in the range o

During their production, single-walled carbon nanotubes form bundles. Owing to the weak van der Waals interaction that holds them together in the bundle, the tubes can easily slide on each other, resulting in a shear modulus comparable to that of graphite. This low shear modulus is also a major obstacle in the fabrication of macroscopic fibres composed of carbon nanotubes. Here, we have introduced stable links between neighbouring carbon nanotubes within bundles, using moderate electron-beam irradiation ins

We measured the elastic modulus of individual multiwalled carbon nanotubes (MWCNTs) grown by catalytic chemical vapor deposition (CVD) over a broad diameter range (10-25 nm). Alternating current (ac) dielectrophoresis was used for efficient tube deposition ...

Background: The in vivo transfer of naked plasmid DNA into organs such as muscles is commonly used to assess the expression of prophylactic or therapeutic genes in animal disease models. Results: In this study, we devised vectors allowing a tight regulatio ...

This study presents the first account for transparent, non-scattering TiO2 films on radio-frequency (RF) plasma and ultraviolet light in the C-spectral region (UVC) pretreated polyethylene (PE). These pretreatments allow the PE to bind higher amounts of Ti ...

We have determined the mechanical anisotropy of a single microtubule by simultaneously measuring the Young's and the shear moduli in vitro. This was achieved by elastically deforming the microtubule deposited on a substrate tailored by electron-beam lithography with a tip of an atomic force microscope. The shear modulus is 2 orders of magnitude lower than the Young's, giving rise to a length-dependent flexural rigidity of microtubules. The temperature dependence of the microtubule's bending stiffness in the

Intermediate filaments (IFs), together with actin filaments and microtubules, compose the cytoskeleton. Among other functions, Ts impart mechanical stability to cells when exposed to mechanical stress and act as a support when the other cytoskeletal filaments cannot keep the structural integrity of the cells. Here we present a study on the bending properties of single vimentin Ts in which we used an atomic force microscopy (AFM) tip to elastically deform single filaments hanging over a porous membrane. We o

Uniformly dispersed deposition of various nanoparticles (gold and silver of 20?50 nm) and nanowires (ZnO and VOx) on different surfaces (Si, GaAs, mica, and steel) was obtained by boiling colloid drops on preheated substrates. Our results suggest a deposit ...

A simple method has been developed for the prodn. of Au surfaces which are uniformly flat over large areas. Si wafers serve as substrates for the evapn. of thin Au layers. A glass slide glued onto the Au film allows successful sepn. of the Au layer from the Si oxide surface. The process leaves a flat Au surface suitable for chem. modification. The surfaces were characterized using scanning force microscopy. An av. roughness of 2.5 .ANG. over areas up to 1000 mm2 was found. Scanning force microscopy images o

Andrzej Kulik

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Diagnosis of muscular dystrophies at the nanometer scale

E. coli Inactivation by High-Power Impulse Magnetron Sputtered (HIPIMS) Cu Surfaces

Nanoscale spatially resolved infrared spectra from single microdroplets

Data coding tools for color-coded vector nanolithography

Mechanical properties of individual microtubules measured using atomic force microscopy

The increase in protein contour length depends on mechanical unfolding conditions

Assessing dystrophies and other muscle diseases at the nanometer scale by atomic force microscopy

Model-Based Estimation of Three-Dimensional Stiffness Parameters in Photonic-Force Microscopy

Infrared nanospectroscopic mapping of a single metaphase chromosome

Photodynamics and surface characterization of TiO2 and Fe2O3 photocatalysts immobilized on modified polyethylene films

Probing fibronectin-antibody interactions using AFM force spectroscopy and lateral force microscopy

Hydrogel Microspheres: Influence of Chemical Composition on Surface Morphology, Local Elastic Properties, and Bulk Mechanical Characteristics

Reinforcement of single-walled carbon nanotube bundles by intertube bridging

Diameter-Dependent Elastic Modulus Supports the Metastable-Catalyst Growth of Carbon Nanotubes

MAR-mediated integration of plasmid vectors for in vivo gene transfer and regulation

Innovative transparent non-scattering TiO2 bactericide thin films inducing increased E. coli cell wall fluidity

Nanomechanics of Microtubules

Exploring the Mechanical Properties of Single Vimentin Intermediate Filaments by Atomic Force Microscopy

Uniformly Dispersed Deposition of Colloidal Nanoparticles and Nanowires by Boiling

Uniformly Flat Gold Surfaces: Imaging the Domain Structure of Organic Monolayers Using Scanning Force Microscopy