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Hubert Girault

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Related publications (430)

Scanning Electrochemical Microscopy as a Readout Tool for Protein Electrophoresis

Scanning electrochemical microscopy (SECM) was used to image silver-stained proteins on a poly(vinylidene difluoride) membrane. The method is based on measuring the current at a scanning microelectrode in the feedback mode. The electrochemical feedback is ...

2007

Capillary Electrophoresis as a Second Dimension to Isoelectric Focusing for Peptide Separation

Hubert Girault, Niels Erik Lion

Capillary zone electrophoresis and carrier ampholytes based capillary electrophoresis have been used as a second separation step to Off-Gel isoelectric focusing for the analysis of complex peptide mixtures. A tryptic digest of four proteins (bovine serum a ...

2007

SECM imaging of MMD-enhanced latent fingermarks

Hubert Girault, Michel Prudent, Christophe Champod

Scanning electrochemical microscopy (SECM) has been used to image latent fingermarks enhanced by adsorption of gold nanoparticles onto which silver is chemically deposited, a process known as ‘‘multi-metal-deposition’’ (MMD). ...

Royal Society of Chemistry2007

On-column conductivity detection in capillary-chip electrophoresis

Hubert Girault

On-column conductivity detection in capillary-chip electrophoresis was achieved by actively coupling the high electric field with two sensing electrodes connected to the main capillary channel through two side detection channels. The principle of this conc ...

2007

3D-ITIES supported on porous reticulated vitreous carbon

Hubert Girault, Nicolas Eugster, Bin Su, Mohamad Hojeij

A thin aqueous film prepared by layer-by-layer assembly of oppositely charged polypeptides on 4-aminobenzoic acid modified porous reticulated vitreous carbon (RVC) electrodes was used with the aim of forming a 3D-ITIES. The film containing a redox mediator ...

2007

Detection of proteins on membranes and in microchannels using copper staining combined with Scanning Electrochemical Microscopy

Hubert Girault, Niels Erik Lion

Scanning Electrochemical Microscopy (SECM) and copper staining are combined to achieve visualisation of proteins on surfaces. Proteins are adsorbed on a polyvinylidene fluoride (PVDF) membrane or on a polyethylene terephthalate (PET) surface and stained us ...

2007

Electrochemical generation of Cu(I) complexes in aqueous solutions studied by on-line mass spectrometry

Hubert Girault, Michel Prudent, Christophe Roussel

Taking advantage of the electrochemical aspects of electrospray ionization (ESI) sources in positive ionization mode mass spectrometry (MS), aqueous copper complexes are electrogenerated using sacrificial copper electrodes. When using 8-hydroxyquinoline or ...

Elsevier2007

Mechanical control of fluids in micro-analytical devices

Hubert Girault, Joël Stéphane Rossier, Joël Rossier, Xiaoxia Bai

An apparatus for manipulating fluid samples comprises a micro-chip device having first and second covered channels, each channel having openings at both ends. The channels intersect to form a common intersection. Three of the openings of the channels are c ...

2006

2-Dimensional Porphyrin Self-Assemblies at Molecular Interfaces

Hubert Girault, Nicolas Eugster, Bin Su

The heterodimer formed by electrostatic association of zinc(II) meso-tetrakis(N-methylpyridyl)porphyrin (ZnTMPyP) and zinc(II) meso-tetrakis(p-sulfonatophenyl)porphyrin (ZnTPPS) exhibits a strong affinity for the interface between water and 1,2-dichloroeth ...

American Chemical Society2006

Antioxidant Redox Sensors Based on DNA Modified Carbon Screen-Printed Electrodes

Hubert Girault, Bin Su

Antioxidant redox sensors based on DNA modified carbon screen-printed electrodes were developed. The carbon ink was doped with TiO2 nanoparticles, onto which double- strand DNA was adsorbed. A redox mediator, namely, tris- 2,2′-bipyridine ruthenium(II) [Ru ...

2006

Adsorption monitoring device

Hubert Girault, Jean-Pierre Abid

A device for monitoring the adsorption of target molecules on a substrate, the device comprising a substrate (1), at least a portion of which is formed as a thin dielectric layer. A first, modified surface (2) of the layer has ionizable groups of the subst ...

2006

Assembly-Controlled Biocompatible Interface on a Microchip: Strategy to Highly Efficient Proteolysis

Hubert Girault

A biocompatible interface was constructed on a microchip by using the layer-by-layer (LBL) assembly of charged polysaccharides incorporating proteases for highly efficient proteolysis. The controlled assembly of natural polyelectrolytes and the enzyme-adso ...

2006

ELECTROWETTING Shake, rattle and roll

Hubert Girault

A comprehensive theoretical basis for understanding electrowetting is now available. It shows that it is possible to effect drastic shape changes in electrolyte droplets immersed in another (immiscible) electrolyte. ...

2006

Multitrack electrospray chips

Hubert Girault, Michel Prudent, Niels Erik Lion, Loïc Dayon, Mélanie Abonnenc

Multitrack electrospray chips (MTEC) were fabricated by UV-photoablation of polyethylene terephthalate (PET) substrates. They are composed of an array of up to six microchannels that are successively used as electrospray ionization (ESI) emitters for mass ...

Wiley-Blackwell2006

CdSe Sensitized Thin Aqueous Films: Probing the Potential Distribution Inside Multilayer Assemblies

Hubert Girault, Nicolas Eugster, Bin Su, Mohamad Hojeij

Ultrathin polypeptide multilayer films are assembled by the sequential electrostatic adsorption of monolayers of poly-L-lysine and poly-L-glutamic acid onto carboxylic acid terminated alkanethiol-modified gold surfaces. The polypeptide multilayer films are ...

American Chemical Society2006

Multilayer-Assembled Microchip for Enzyme Immobilization as Reactor Toward Low-Level Protein Identification

Hubert Girault

A microchip reactor has been developed on the basis of a layer-by-layer approach for fast and sensitive digestion of proteins. The resulting peptide analysis has been carried out by matrix-assisted laser desorption ionization timeof- flight mass spectromet ...

2006

Solvent Effect on Redox Properties of Hexanethiolate Monolayer-Protected Gold Nanoclusters

Hubert Girault, Bin Su

The capacitance of monolayer-protected gold nanoclusters (MPCs), CMPC, in solution has been theoretically reconsidered from an electrostatic viewpoint, in which an MPC is considered as an isolated charged sphere within two dielectric layers, the intrinsic ...

2006

Nanowires Networks for Biomolecular Detection using Contactless Impedance Tomoscopy Technique

Jean-Philippe Ansermet, Hubert Girault, Jean-Pierre Abid

Here we present the detection of ultralow concentrations of biomolecules in a device made from a polycarbonate membrane containing a network of gold nanowires and using a “contactless” impedance tomoscopy technique. The sensor comprises a thin dielectric l ...

2006

Role of Adsorbates on Dynamics of Hot-Electron (type I and II) Thermalization within Gold Nanoparticles

Hubert Girault, Jean-Pierre Abid, Christophe Bauer

Early stages of hot-electron thermalization in small gold nanoparticles wrapped in an adsorbates shell have been investigated by femtosecond transient absorption spectroscopy. Type-I hot electrons thermalize in 800 fs (to form type-II hot electron) either ...

Elsevier2006

Probing Cysteine Reactivity in Proteins by Mass Spectrometric EC-Tagging

Hubert Girault, Christophe Roussel, Loïc Dayon

The on-line electrochemical tagging (EC-tagging) of cysteine residues in proteins during mass spectrometry is studied to probe the cysteine environment. Benzoquinone probes electrogenerated at a microspray electrode react with the thiol functions of the pr ...

American Chemical Society2006

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