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Hubert Girault

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Related publications (430)

Immobilized pH Gradient Gel Cell To Study the pH Dependence of Drug Lipophilicity

Hubert Girault, Christophe Roussel, Hoang-Trang Lam, Carlos Manuel De Melo Pereira

An experimental setup to study the pH dependence of standard ion-transfer potentials at the water/NPOE interface is presented. The system is composed of a micro-hole generated by laser photoablation in a 12-μm polyethylene terephthalate film, the aqueous p ...

2006

Hot Adsorbate-Induced Retardation of the Internal Thermalization of Nonequilibrium Electrons in Adsorbate-Covered Metal Nanoparticles

Hubert Girault, Jean-Pierre Abid, Christophe Bauer

Femtosecond transient absorption spectroscopy has been used to investigate the electron-electron scattering dynamics in sulfate-covered gold nanoparticles of 2.5 and 9.2 nm in diameter. We observe an unexpected retardation of the absolute internal thermali ...

2006

Microscale total analysis system

Hubert Girault

The invention relates to an apparatus for performing chemical assays in an aqueous medium. The apparatus contains a reaction chamber(s) and a liquid in-flow channel connected to each chamber. The flow of liquid through the fluid in-flow channel to the reac ...

2005

Chip electrospray mass spectrometry for carbohydrate analysis

Hubert Girault, Niels Erik Lion

Currently two types of chip systems are used in conjunction with MS: out-of-plane devices, where hundreds of nozzles, nanospray emitters are integrated onto a single silicon substrate from which electrospray is established perpendicular to the substrate, a ...

2005

Thin chip microsprayer system coupled to quadrupole time-of-flight mass spectrometer for glycoconjugate analysis

Hubert Girault, Joël Stéphane Rossier, Niels Erik Lion

A thin chip polymer-based microsprayer has been coupled to a hybrid quadrupole time-of-flight mass spectrometer (QTOF MS) and introduced in carbohydrate research. The feasibility of the approach is demonstrated for mapping, sequencing and structural elucid ...

Royal Society of Chemistry2005

Study of peptide on-line complexation with transition-metal ions generated from sacrificial electrodes in thin-chip polymer microsprays

Tatiana Rohner, Hubert Girault

A miniaturized polymer electrospray-type interface is used to study metal-ion chelation with model peptides. Taking advantage of the intrinsic electrochemical behavior of electrospray, a sacrificial electrode is used to generate at the same time electrospr ...

Wiley-Blackwell2005

Editorial: Microfluidics in system biology

Hubert Girault, Joël Stéphane Rossier, Niels Erik Lion

2005

Size Dependence of Investigations of Hot Electron Cooling Dynamincs in Metal/Adsorbates Nanoparticles

Hubert Girault, Jean-Pierre Abid, Christophe Bauer

The size dependence of electron-phonon coupling rate has been investigated by femtosecond transient absorption spectroscopy for gold nanoparticles (NPs) wrapped in a shell of sulfate with diameter varying from 1.7 to 9.2 nm. Broad-band spectroscopy gives a ...

Elsevier2005

Reactivity of Monolayer-Protected Gold Nanoclusters at Dye-Sensitized Liquid/Liquid Interfaces

Hubert Girault, Nicolas Eugster, Bin Su

Hexanethiolate monolayer-protected gold nanoclusters (MPCs) were used as redox quenchers at the polarizable water/1,2-dichloroethane (DCE) interface. Photocurrent responses originating from the heterogeneous quenching of photoexcited water soluble porphyri ...

2005

Dynamic protein adsorption in microchannels by "stop-flow" and continuous flow

Hubert Girault, Andrea Lionello

This work addresses two ways of loading proteins on microchannel surfaces for immunoassay applications: the “stop-flow” and the continuous flow processes. The “stop-flow” method consists of successive static incubation periods where the bulk solution deple ...

2005

Numerical Simulation of Two-Phase Partition Chromatography in Microchannels for Moderated Log P Measurements

Hubert Girault, Pierre-Alain Carrupt

A finite element simulation has been used in order to study the partition chromatography process of one species between an aqueous mobile phase and an organic stationary phase located at the bottom of a rectangular microchannel. The transient model incorpo ...

Elsevier2005

Antioxidant Sensors Based on DNA-Modified Electrodes

Hubert Girault, Christophe Roussel

TiO2/ITO modified electrodes were developed to quantitatively photooxidize adsorbed ds-DNA and to study the effect of antioxidants as ds-DNA protecting agents. TiO2 films are used for efficient ds-DNA immobilization, for ds- DNA oxidation through photogene ...

2005

Protein adsorption in static microsystems: effect of the surface to volume ratio

Hubert Girault, Andrea Lionello

A numerical model for the adsorption kinetics of proteins on the walls of a microchannel has been developed using the finite element method (FEM) to address the coupling with diffusion phenomena in the restricted microchannel volume. Time evolutions of the ...

Royal Society of Chemistry2005

Copper staining/labeling and scanning electrochemical microscopy readout of proteins on poly(vinylidene difluoride) membranes

Hubert Girault, Niels Erik Lion

Scanning Electrochemical Microscopy (SECM) is used in combination with copper labeling to visualize roteins on surfaces. Proteins are adsorbed on a poly(vinylidene difluoride) (PVDF) membrane and stained using a standard protocol involving copper salts. Th ...

2005

Simulations of the adsorption of ionic species at polarisable liquid liquid interfaces

Hubert Girault, Nicolas Eugster, Bin Su

The adsorption of ions at the interface between two immiscible electrolyte solutions (ITIES) is primarily controlled by the potential distribution across the interface, which in turn is influenced by the adsorption process. In the present paper, we simulat ...

2005

Salt Removal During Off-GelTM Electrophoresis of Protein Samples

Hubert Girault, Niels Erik Lion, Christophe Roussel

The Off-Gel™ technology was recently described for protein fractionation in a solution placed on top of an immobilized pH gradient gel. In addition, this process was found to remove salts from the biological samples to analyze. This desalting effect is stu ...

2005

Absolute Standard Redox Potential of Monolayer-Protected Gold Nanoclusters

Hubert Girault, Bin Su

The redox properties of monolayer-protected gold nanoclusters (MPCs) are considered from both the theoretical and experimental viewpoints. The “absolute standard redox potential” ([ ]abs) of MPCs is first derived from electrostatic considerations. A linear ...

2005

Supercapacitive Admittance Tomoscopy

Hubert Girault, Jean-Pierre Abid

A sensor for measuring adsorption on a substrate has been designed including a contactless detection scheme, called supercapacitive admittance tomoscopy (SCAT). The sensor comprises a thin dielectric layer with two parallel band electrodes on the one side ...

2005

On-Line Counting of Cysteine Residues in Peptides during Electrospray Ionization by Electrogenerated Tags and Their Application to Protein Identification

Hubert Girault, Michel Prudent, Niels Erik Lion, Christophe Roussel, Loïc Dayon

The electrochemically induced mass spectrometric tagging of cysteines by substituted hydroquinones was studied for peptides in a classical electrospray solvent (i.e., MeOH/H2O/AcOH 50/49/1). The tagging efficiency was tested with different hydroquinone com ...

2005

Adsorption and photoreactivity of CdSe nanoparticles at liquid|liquid interfaces

Hubert Girault, Jean-Pierre Abid, Nicolas Eugster, Bin Su

The voltage induced assembly and photoreactivity of cadmium selenide (CdSe) nanoparticles protected by mercaptosuccinic acid are studied at the polarisable interface between water and 1,2-dichloroethane electrolyte solutions. Cyclic voltammograms and admit ...

2005

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