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Hubert Girault

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Related publications (430)

Microfabricated Dual Sprayer for On-Line Mass Tagging of Phosphopeptides

Hubert Girault, Joël Stéphane Rossier, Michel Prudent, Niels Erik Lion

Phosphopeptides tagging reactions by dinuclear zinc(II) complexes (1,3-bis[bis(2-pyridylmethyl)amino]-propan- 2-olato dizinc(II)3+, called tag) were performed with a dual-channel microsprayer in electrospray ionization mass spectrometry. The reaction is fi ...

2008

Plate for matrix-assisted laser desorption ionization mass spectrometry for, e.g. analyzing peptide, has substrate that is covered with light sensitive matrix with light absorber, charge carrier, probe molecule and photo-sensitizer

Hubert Girault, Niels Erik Lion, Christophe Roussel, Liang Qiao

NOVELTY - The plate has electrically conductive substrate (1) that is covered with light sensitive matrix (2). The matrix comprises light absorber, charge carrier, probe molecule and photo-sensitizer (3) arranged to oxidize probe molecule by irradiation of ...

2008

Capillary electrophoresis immunoassay using magnetic beads

Hubert Girault, Anne-Laure Gassner, Hong-Xu Chen

Protein A-coated magnetic beads (0.3 μm) have been trapped in a small portion of a neutrally coated capillary (50 μm id). Anti-β-lactoglobulin (β-LG) antibodies have then been immobilized on the beads through strong affinity with protein A to subsequently ...

2008

H2O2 generation by decamethylferrocene at a liquid | liquid interface

Hubert Girault, Michel Prudent, Zdenek Samec, Bin Su, Mohamad Hojeij, Raheleh Partovi Nia, Anne-Clémence Corminboeuf

Hydrogen peroxide generation at a liquid|liquid interface occurs with a yield of 20 % with respect to the concentration of reducing agent (decamethylferrocene). The liquid|liquid interface supplies electrons from the reducing agent and protons from the aqu ...

Wiley-VCH Verlag GmbH2008

In-source photocatalytic reduction of disulfide bonds during laser desorption ionization

Hubert Girault, Hongyan Bi, Liang Qiao

A photosensitive plate based on sintered TiO2 nanoparticles has been developed to carry out in-source photo-induced reductions for cleavage of disulfide bridges using glucose as a hole scavenger during laser desorption ionization. ...

2008

Nanoporous photocathode and photoanode for light energy conversion

Hubert Girault, Bin Su, Mohamad Hojeij

2008

Generation of OH radicals at palladium oxide nanoparticle modified electrodes, and scavenging by fluorescent probes and antioxidants

Hubert Girault, Junqiu Liu

The reduction of palladium oxide nanoparticles in the presence of hydrogen peroxide or dissolved oxygen gives rise to a catalytic reduction current dependent on the hydrogen peroxide concentration or the oxygen concentration. Using terephthalic acid as an ...

2008

On-line Electrogeneration of Copper-Peptide Complexes in Microspray Mass Spectrometry

Hubert Girault, Michel Prudent

The interaction of copper ions with peptides was investigated by electrospray mass spectrometry. Two electrospray micro-emitters were compared, the first one with a platinum electrode using a copper(II) electrolyte solution containing a peptide sample, and ...

2008

Porphyrin ‘‘Mille-Feuilles” photo-electrodes

Hubert Girault, Bin Su, Mohamad Hojeij

A porphyrin ‘‘Mille-Feuilles” photo-electrode is fabricated layer-by-layer by deposition of anionic [zinc meso-tetrakis(p-sulfonato phenyl) porphyrin]4- (ZnTPPS4−) and positively charged polypeptides on a 11-mercaptoundecanoic acid-modified gold electrode ...

2008

Biphasic Electrospray Ionization for the Study of Interfacial Complexes

Hubert Girault, Michel Prudent, Manuel Alejandro Méndez Agudelo

Biphasic electrospray ionization (BESI) mass spectrometry is achieved by using a dual-channel microsprayer, where channels filled with different immiscible phases meet at the Taylor cone. Two types of interfacial complexation reactions have been studied: t ...

The Japan Society of Analytical Chemistry / The Japan Society for Analytical Chemistry2008

Peptide-Phospholipid Complex Formation at Liquid-Liquid Interfaces

Hubert Girault, Michel Prudent, Bin Su, Manuel Alejandro Méndez Agudelo

Two peptides known to interact with receptors embedded in cell membranes, angiotensin III (AngIII) and Leuenkephalin(LeuEnk), were studied electrochemically at the interface formed between two immiscible electrolyte solutions modified by an adsorbed monola ...

2008

SECM photography

Hubert Girault, Bin Su, Mohamad Hojeij, Fernando Cortes Salazar

A novel strategy for imaging commercial black-and-white negative photo films by scanning electrochemical microscopy (SECM) is demonstrated. The imaging is based on using IrCl3 6 as the redox mediator to dissolve silver grains. The SECM probe is biased at a ...

Elsevier2008

Electrokinetic supercharging for highly efficient peptide preconcentration in capillary zone electrophoresis

Hubert Girault

Electrokinetic supercharging has been integrated in CZE for the development of a highly sensitive methodology for protein tryptic digest analysis. A careful choice of the experimental conditions led to sensitivity enhancement factors between 1000 and 10 00 ...

2008

Protoporphyrin IX sensitized titanium oxide gel electrode

Hubert Girault, Christophe Roussel, Bin Su

Titanium oxide (Ti(O)) xerogel films functionalized by protoporphyrin IX (PPIX) and ferrocene carboxylic acid (FCA) were deposited on indium tin oxide (ITO) electrodes following a sol–gel synthesis. PPIX and FCA were first complexed to titanium oxide precu ...

Elsevier2008

Specific On-Plate Enrichment of Phosphorylated Peptides for Direct MALDI-TOF MS Analysis

Hubert Girault, Christophe Roussel, Jingjing Wan, Liang Qiao

An on-plate specific enrichment method is presented for the direct analysis of peptides phosphorylation. An array of sintered TiO2 nanoparticle spots was prepared on a stainless steel plate to provide porous substrate with a very large specific surface and ...

American Chemical Society2007

Controlled Reversible Adsorption of Core-Shell Metallic Nanoparticles at the Polarized Water/1,2-Dichloroethane Interface Investigated by Optical Second-Harmonic Generation

Pierre-François Marie Brevet, Hubert Girault, Jean-Pierre Abid, Christophe Bauer

We report the observation of the reversible adsorption of core-shell gold-silver nanoparticles at the polarized water/1,2-dichloroethane interface using the nonlinear optical technique of surface second-harmonic generation. This study unambiguously demonst ...

2007

Gel-free IEF in a membrane-sealed multicompartment cell for proteome prefractionation

Hubert Girault, Hoang-Trang Lam

A minidevice for performing gel-free proteome prefractionation via conventional IEF in soluble carrier ampholyte buffers is reported here. It consists of a compact block of poly-oxymethylene in which eight samples and two electrode chambers are machined. E ...

2007

Diagonal chromatographic selection of cysteinyl peptides modified with benzoquinones

Hubert Girault, Loïc Dayon

The derivatization of cysteine-containing peptides with benzoquinone compounds is rapid, quantitative and specific in acidic media. The conversion of cysteines into hydrophobic benzoquinone-adducted residues in peptides is used here to alter the chromatogr ...

Springer Verlag2007

Modeling the Isoelectric Focusing of Peptides in an OFFGEL Multicompartment Cell

Hubert Girault, Niels Erik Lion, Hoang-Trang Lam

In proteomic analysis of complex samples at the peptide level (termed shotgun proteomics), an effective prefractionation is crucial to decrease the complexity of the peptide mixture for further analysis. In this perspective, the high-resolving power of the ...

American Chemical Society2007

Fingerprint imaging by scanning electrochemical microscopy

Hubert Girault

An efficient strategy for visualizing human fingerprints on a poly(vinyldene difluoride) membrane (PVDF) by sanning electrochemical microscopy (SECM) has been developed. Compared to a classical ink fingerprint image, here the ink is replaced by an aqueous ...

Elsevier2007

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