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This lecture covers the Fano factor, which quantifies the difference in energy resolution in ionization-based detectors. It explains how the Fano factor is derived and its significance in balancing fluctuations in energy dissipation. The comparison between different detector types, such as semiconductors, ionization chambers, and scintillators, is also discussed, highlighting the superior resolution power of semiconductors. Statistical fluctuations and the impact on resolution power are analyzed through examples and spectra comparisons.