Generation of a Large Peptide Phage Display Library by Self-Ligation of Whole-Plasmid PCR Product

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Seventh pandemic Vibrio cholerae strains contain two pathogenicity islands that encode the DNA defense modules DdmABC and DdmDE. Here we use cryogenic electron microscopy to reveal the mechanistic basis for plasmid defense by DdmDE. A structure of the DdmD ...

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Bacteria are ubiquitous single cellular organisms. Compared to eukaryotic cells, bacteria have two unique characteristics: the membrane-less nucleoid and the cell wall built of peptidoglycan (PG). In most bacteria, a single circular chromosome is compacted ...

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Scaffold-based protein libraries are designed to be both diverse and rich in functional/folded proteins. However, introducing an extended diversity while preserving stability of the initial scaffold remains a challenge. Here we developed an original approa ...

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Shearing DNA to a certain size is the first step in many medical and biological applications, especially in next-generation gene sequencing technology. In this article, we introduced a highly efficient ultrasonic DNA fragmentation method enhanced by needle ...

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The bacteriophage T4 genome contains two genes that code for proteins with lysozyme activity-e and 5. Gene e encodes the well-known T4 lysozyme (commonly called T4L) that functions to break the peptidoglycan layer late in the infection cycle, which is requ ...

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Adeno-associated virus (AAV) vectors are currently among the most commonly applied for in vivo gene therapy approaches. The evaluation of vectors during clinical development requires the production of considerable amounts of highly pure and potent vectors. ...

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The specific interaction between DNA and proteins constitutes one of the crucial elements in the regulation of gene expression. This thesis focuses on the development and optimization of two microfluidic-based technologies called SMiLE-seq and FloChIP that ...

Generation of a Large Peptide Phage Display Library by Self-Ligation of Whole-Plasmid PCR Product

Chattez avec Graph Search

Molecular mechanism of plasmid elimination by the DdmDE defense system

Advancing Cell-Free Synthetic Biology: From sustained PURE protein synthesis to an isothermal one-pot DNA replication system

Resolving bacterial cell biology: from replisome dynamics to cell wall synthesis

Development of cysteine cathepsin inhibitors combined with cell type-specific delivery for the treatment of B-cell lymphoma and solid tumors

Recycling of Polymerase Chain Reaction (PCR) Kits

Design of an artificial phage-display library based on a new scaffold improved for average stability of the randomized proteins

Development of multiplexed single-molecule imaging techniques to study chromatin invasion dynamics of chromatin effector proteins

A field-capable rapid plant DNA extraction protocol using microneedle patches for botanical surveying and monitoring

Electronic readout of DNA amplification in nanoliter chambers. Strategies towards highly parallel semiconductor-based nucleic acid amplification testing

The enhancement of DNA fragmentation in a bench top ultrasonic water bath with needle-induced air bubbles: Simulation and experimental investigation

Polydimethylsiloxane microstructure-induced acoustic streaming for enhanced ultrasonic DNA fragmentation on a microfluidic chip

Directed evolution of DNA-wrapped single-walled carbon nanotube complexes for optical sensing

Development of Covalent Chitosan-Polyethylenimine Derivatives as Gene Delivery Vehicle: Synthesis, Characterization, and Evaluation

Establishing a Ternary System for Optical Monitoring of DNA-Protein Interactions with Single-Walled Carbon Nanotubes

Structure and Function of the T4 Spackle Protein Gp61.3

Site-Specific Protein Conjugation onto Fluorescent Single-Walled Carbon Nanotubes

High-throughput microfluidic platforms for characterizing and engineering cell-free gene regulatory circuits

Scalable Production of AAV Vectors in Orbitally Shaken HEK293 Cells

Single-molecule sensing of peptides and nucleic acids by engineered aerolysin nanopores

Integrated microfluidic tools for the characterization of protein/DNA interactions in vitro and in vivo