Generation of a Large Peptide Phage Display Library by Self-Ligation of Whole-Plasmid PCR Product

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The DNA Curtains assay is a recently developed experimental platform for protein-DNA interaction studies at the single-molecule level that is based on anchoring and alignment of DNA fragments. The DNA Curtains so far have been made by using chromium barrie ...

In this study, the effect of MS2 bacteriophage on the inactivation of its Escherichia coli host was evaluated in an effort to recreate some the main factors governing solar disinfection of wastewater (namely microbial growth, infection, photonic flux). As ...

Recent progress in protein engineering, empowered by the rapidly evolving fields of synthetic biology and DNA synthesis, has enabled the generation of various custom-designed proteins. Their thorough analysis has led to applications in areas including medi ...

Introduction. Oligochaetes are recognized as valuable bioindicators of sediment quality in streams and lakes. The development of an oligochaete index based on the identification of specimens using DNA barcodes requires a method for simultaneously preservin ...

The isothermal amplification of DNA in minimally buffered conditions allows to perform and monitor nucleic acid amplification with minimal technological and operative requirements. We show in this work how phi29 can operate multiple displacement amplificat ...

Bacteriophages of the myoviridae family, R-type pyocin, Photorhabus virulence cassette (PVC), Serratia antifeeding prophage (Afp) and type 6 secretion system (T6SS) form a class of contractile injection systems that share common structural and functional c ...

Phage display relies on a bacterial infection step in which the phage particles are replicated to perform multiple affinity selection rounds and to enable the identification of isolated clones by DNA sequencing. While this process is efficient for wild-typ ...

Cyclic peptide ligands are a promising molecular format for the development of therapeutics. They combine some of the advantages of large protein therapeutics (high affinity and specificity) and of small molecule drugs (accessibility to chemical synthesis ...

Cyclic peptides can bind to protein targets with high affinities and selectivities, which makes them an attractive modality for the development of research reagents and therapeutics. Additional properties, including low inherent toxicity, efficient chemica ...

Untargeted plasmid integration into mammalian cell genomes remains a poorly understood and inefficient process. The formation of plasmid concatemers and their genomic integration has been ascribed either to non-homologous end-joining (NHEJ) or homologous r ...

The ability to electronically interface living cells with electron accepting scaffolds is crucial for the development of next-generation biophotovoltaic technologies. Although recent studies have focused on engineering synthetic interfaces that can maximiz ...

Generation of a Large Peptide Phage Display Library by Self-Ligation of Whole-Plasmid PCR Product

Chattez avec Graph Search

Fixed DNA Molecule Arrays for High-Throughput Single DNA-Protein Interaction Studies

E-coli-MS2 bacteriophage interactions during solar disinfection of wastewater and the subsequent post-irradiation period

Development of microfluidic tools and methods for the biochemical characterization of scFv and Cas9 affinity reagents and their applications in molecular detection

Simultaneous preservation of the DNA quality, the community composition and the density of freshwater oligochaetes for the development of genetically based biological indices

Evaluation of Auramine O staining and conventional PCR for leprosy diagnosis: A comparative cross-sectional study from Ethiopia

Isothermal multiple displacement amplification of DNA templates in minimally buffered conditions using phi29 polymerase

Structural and functional analysis of host attachment proteins from contractile injection systems

Bypassing bacterial infection in phage display by sequencing DNA released from phage particles

New methods for developing (bi)cyclic peptides by phage display

Phage Selection of Cyclic Peptides for Application in Research and Drug Development

MAR-Mediated transgene integration into permissive chromatin and increased expression by recombination pathway engineering

A synthetic biology approach to engineering living photovoltaics