Monitoring alpha-synuclein oligomerization and aggregation using bimolecular fluorescence complementation assays: What you see is not always what you get

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Mitochondria are essential organelles participating in numerous cellular functions, including energy harvesting, regulation of homeostasis and apoptosis. Changes in mitochondrial number, morphology, and function not only impact cellular metabolism but also ...

Redox homeostasis is a key factor in maintaining cellular function and health. The main determinant of the intracellular redox potential is ubiquitous glutathione (GSH) together with its oxidized dimer (GSSG). Importantly, both redox equilibrium and GSH ho ...

The invention provides microfluidic screening systems for the identification of compounds or compositions influencing cellular transcriptomes. The invention is predicated upon taking into account differences in newly synthesized mRNA in contrast to so call ...

Cells are the smallest operational units of living systems. Through synthesis of various biomolecules and exchange of signals with the environment, cells tightly regulate their composition to realize a specific functional state. The transformation of a cel ...

Cis-genetic effects are key determinants of transcriptional divergence in discrete tissues and cell types. However, how cis- and trans-effects act across continuous trajectories of cellular differentiation in vivo is poorly understood. Here, we quantify al ...

Analysis of single-cell datasets generated from diverse organisms offers unprecedented opportunities to unravel fundamental evolutionary processes of conservation and diversification of cell types. However, interspecies genomic differences limit the joint ...

The invention relates to a fluorometer system for analyzing fluid samples comprising a plurality of particles capable of being labeled with at least two fluorescent labels, in particular two distinct fluorophores. The invention also relates to a fluorescen ...

Macrocycles provide an attractive modality for drug development but the identification of ligands to targets of interest is hindered by the lack of large macrocyclic compound libraries for high-throughput screening. A strategy to efficiently synthesize lar ...

Networks of tryptophan (Trp)& horbar;an aromatic amino acid with strong fluorescence response & horbar;are ubiquitous in biological systems, forming diverse architectures in transmembrane proteins, cytoskeletal filaments, subneuronal elements, photorecepto ...

Traditional cell cultures have long been fundamental to biological research, offering an alternative to animal models burdened by ethical constraints and procedural intricacies, often lacking relevance to human physiology and disease. Moreover, their inabi ...

Significance: Fluorescence guidance is used clinically by surgeons to visualize anatomical and/or physiological phenomena in the surgical field that are difficult or impossible to detect by the naked eye. Such phenomena include tissue perfusion or molecula ...

As the fundamental machinery orchestrating cellular functions, proteins influence the state of every cell profoundly. As cells exhibit significant variations from one to another, analyzing the proteome on a single-cell level is imperative to unravel their ...

Fluorescent probes are an indispensable tool in the realm of bioimaging technologies, providing valuable insights into the assessment of biomaterial integrity and structural properties. However, incorporating fluorophores into scaffolds made from melt elec ...

High-throughput transcriptomics is of increasing fundamental biological and clinical interest. The generation of molecular data from large collections of samples, such as biobanks and drug libraries, is boosting the development of new biomarkers and treatm ...

Serial crystallography at X-ray free-electron lasers (XFELs) permits the determination of radiation-damage free static as well as time-resolved protein structures at room temperature. Efficient sample delivery is a key factor for such experiments. Here, we ...

Investigating the dynamic activities of protein expression and signaling in living organisms is a crucial focus of intense research aimed at elucidating the processes that underlie disease progression and improving treatments and drug development. Resolvin ...

This manuscript proposes a new dual-mode cell imaging system for studying the relationships between calcium dynamics and the contractility process of cardiomyocytes derived from human-induced pluripotent stem cells. Practically, this dual-mode cell imaging ...

Retinoblastoma is a rare childhood cancer of the eye. Of the small number of drugs are used to treat retinoblastoma, all have been repurposed from drugs developed for other conditions. In order to find drugs or drug combinations better suited to the improv ...

The present invention relates to a method for analyzing cellular responsiveness to hormones or drags in a tissue sample based on an alginate-based 3D ex vivo culture system for normal and malignant tissue microstructures and uses thereof. ...

These datasets contain the total particle number concentrations and normalized size distributions (dN/dlogDp) of excited, fluorescent, and hyper-fluorescent particles of sizes 0.5 to 20 μm (optical diameter). The normalized size distribution datasets are s ...

Monitoring alpha-synuclein oligomerization and aggregation using bimolecular fluorescence complementation assays: What you see is not always what you get

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Drug Screening in C. elegans to identify new pharmacological mitochondrial stress inducers

HaloTag-Based Tools for Live-Cell Imaging and Elucidation of Organellar Redox Biology

Improved microfluidic transcriptome based screening methods

Deciphering the nature of cell state transitions in single cells using quantitative modeling of temporal dynamics

The dynamic genetic determinants of increased transcriptional divergence in spermatids

Toward universal cell embeddings: integrating single-cell RNA-seq datasets across species with SATURN

Fluorometer system, fluorescene analysis method, and use thereof

Methods for high-throughput synthesis and screening of peptide libraries

Ultraviolet Superradiance from Mega-Networks of Tryptophan in Biological Architectures

Accessible and highly observable gastrointestinal organoid model systems for studying host-pathogen interactions

Subsurface fluorescence time-of-flight imaging using a large-format single-photon avalanche diode sensor for tumor depth assessment

Development of tools and methods for protein identification: From single molecules to in vivo applications

[n]Cycloparaphenylenes as Compatible Fluorophores for Melt Electrowriting

Multi-well plate lid for single-step pooling of 96 samples for high-throughput barcode-based sequencing

A multi-reservoir extruder for time-resolved serial protein crystallography and compound screening at X-ray free-electron lasers

Label-free plasmonic microarray for multiplexed analysis of cells and tumor organoids

Automated Dual-Mode Cell Monitoring To Simultaneously Explore Calcium Dynamics and Contraction-Relaxation Kinetics within Drug-Treated Stem Cell-Derived Cardiomyocytes

The predictive capacity of in vitro preclinical models to evaluate drugs for the treatment of retinoblastoma

Method for hormone or drug screening in a tissue sample

Wideband integrated bioaerosol sensor (WIBS) excited, fluorescent, and hyper-fluorescent particle number concentrations and normalized size distributions (dN/dlogDp) measured in the Swiss container during MOSAiC 2019/2020