Protocol for live imaging of intracellular nanoscale structures using atomic force microscopy with nanoneedle probes

À propos
Confidentialité
Mentions légales

Graph Chatbot

Publications associées (32)

Method and system for determining intracellular mediators' activity

Horst Vogel, Philippe Renaud, Thamani Dahoun, Margaux Catherine Marie Duchamp, Marc Brugarolas Campillos

A method for determining the presence of a molecule coupled to the cytoplasmic side of a cellular membrane is disclosed. The method is implemented in a microfluidic setting and is particularly suitable for determining the presence and/or the activity of a ...

2022

Controlling motion at the nanoscale with light

Mintae Chung

Optical manipulation at the micro- nano-scale is a fascinating topic due to its inherent non-invasive properties and multifaceted applications in various fields such as biology, sensing, micro-fluidics, and micro- nano-robotics. This thesis involves intens ...

EPFL2022

Preparation and Analysis of Bacteria Surface-Modified with Polymer Nanoparticles

Lisa Patricia Elisabeth Bader

Cell-based drug carriers present an interesting approach to improve the targeted delivery of therapeutic drugs in vivo. Specifically, bacterial cells possess interesting properties such as motility and sensing that allow some strains to selectively target ...

EPFL2022

Live-seq enables temporal transcriptomic recording of single cells

Bart Deplancke, Pernille Yde Rainer, Riccardo Dainese, Wanze Chen, Magda Zachara, Vincent Roland Julien Gardeux, Wouter Saelens, Orane Josiane Guillaume-Gentil

Single-cell transcriptomics (scRNA-seq) has greatly advanced our ability to characterize cellular heterogeneity(1). However, scRNA-seq requires lysing cells, which impedes further molecular or functional analyses on the same cells. Here, we established Liv ...

NATURE PORTFOLIO2022

Strategic Insights into Engineering Parameters Affecting Cell Type-Specific Uptake of DNA-Based Nanomaterials

Maartje Martina Cornelia Bastings, Alice Comberlato, Hugo José Rodriguez Franco, Marianna Mainardi Koga

DNA-based nanomaterials are gaining popularity as uniform and programmable bioengineering tools as a result of recent solutions to their weak stability under biological conditions. The DNA nanotechnology platform uniquely allows decoupling of engineering p ...

American Chemical Society2022

Mass transport of water vapor and ions from Å-scale graphene nanopores

Wan-Chi Lee

Hydrodynamics at the nanoscale involves both fundamental study and application of fluid and mass transport phenomena in nanometer-sized confinements. Nanopores in single-layer graphene can be highly attractive for exploring the molecular transport of gas a ...

EPFL2022

Engineering synthetic adhesins for biophysical investigation and rewiring of host-microbe interactions

Xavier Jean-Yves Pierrat

Host attachment is often a critical step in the onset of pathogenesis. To attach to host cells, bacteria have evolved a range of adhesins that bind to specific receptors. Some of these adhesins have been thoroughly characterized using biochemical technique ...

EPFL2022

Visualizing intracellular nanostructures of living cells by nanoendoscopy-AFM

Marcos Penedo Garcia

Atomic force microscopy (AFM) is the only technique that allows label-free imaging of nanoscale biomolecular dynamics, playing a crucial role in solving biological questions that cannot be addressed by other major bioimaging tools (fluorescence or electron ...

AMER ASSOC ADVANCEMENT SCIENCE2021

Biotin-NeutrAvidin Mediated Immobilization of Polymer Micro- and Nanoparticles on T Lymphocytes

Harm-Anton Klok, Olivier Burri, Maxime Ayer, Arne Seitz, Romain Guiet

Cells are powerful carriers that can help to improve the delivery of nanomedicines. One approach to use cells as carriers is to immobilize the nanoparticulate cargo on the cell surface. While a plethora of chemical conjugation strategies are available to b ...

2021

Nanomechanical bio-sensing for fast and reliable detection of viability and susceptibility of microorganisms

Giovanni Dietler, Raphaël Foschia, Sandor Kasas, María Inés Villalba, Leonardo Venturelli

The metabolic activity of living organisms results in cellular motion on a nanometric scale that can be efficiently detected by micro- and nano- fabricated sensors. Quartz crystal microbalance and atomic force microscopy (AFM) inspired techniques have rece ...

2021

Quantum Sensing of Free Radicals in Primary Human Dendritic Cells

Mayeul Sylvain Chipaux

Free radicals are crucial indicators for stress and appear in all kinds of pathogenic conditions, including cancer, cardiovascular diseases, and infection. However, they are difficult to detect due to their reactivity and low abundance. We use relaxometry ...

AMER CHEMICAL SOC2022

Cell penetration efficiency analysis of different atomic force microscopy nanoneedles into living cells

Marcos Penedo Garcia

Over the last decade, nanoneedle-based systems have demonstrated to be extremely useful in cell biology. They can be used as nanotools for drug delivery, biosensing or biomolecular recognition inside cells; or they can be employed to select and sort in par ...

Nature Research2021

Page 2 sur 2