Helical-Track Bioreactors for Bacterial, Mammalian and Insect Cell Cultures

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Due to conservation of neuronal functioning across phyla, molecular targets of insecticides are similar in insects and vertebrates. Insecticides thus pose a risk to aquatic vertebrates, such as fish, and potentially cause neurotoxic effects. Although these ...

Spiroplasma is a genus of Mollicutes whose members include plant pathogens, insect pathogens and endosymbionts of animals. Spiroplasma phenotypes have been repeatedly observed to be spontaneously lost in Drosophila cultures, and several studies have docume ...

The use of modern molecular biology tools and simple but powerful tractable model organisms such as Drosophila has contributed significantly to our recent advances in the innate immunity field, notably phagocytosis. Since 2001, many phagocytic transmembran ...

Flight through cluttered environments, such as forests, poses great challenges for animals and machines alike because even small changes in flight path may lead to collisions with nearby obstacles. When flying along narrow corridors, insects use the magnit ...

Flying robots are increasingly used for tasks such as aerial mapping, fast exploration, video footage and monitoring of buildings. Autonomous flight at low altitude in cluttered and unknown environments is an active research topic because it poses challen ...

Eater and NimC1 are transmembrane receptors of the Drosophila Nimrod family, specifically expressed in haemocytes, the insect blood cells. Previous ex vivo and in vivoRNAi studies have pointed to their role in the phagocytosis of bacteria. Here, we have cr ...

Adeno-associated virus (AAV) vectors are currently among the most commonly applied for in vivo gene therapy approaches. The evaluation of vectors during clinical development requires the production of considerable amounts of highly pure and potent vectors. ...

A device for sorting biological entities is disclosed. The device comprises a channel to canalize the biological entities and a selector having slots to accommodate said entities. Means for detecting and analysing an optical parameter of the biological ent ...

Origami manufacturing has led to considerable advances in the field of foldable structures with innovative applications in robotics, aerospace, and metamaterials. However, existing origami are either load-bearing structures that are prone to tear and fail ...

Many insect species are associated with endosymbiotic bacteria which have the particularity of living within host tissues. Endosymbionts benefit from this stable and nutritious environment, while providing ecological advantages to their host, such as prote ...

In most well-studied rod-shaped bacteria, peptidoglycan is primarily crosslinked by penicillin-binding proteins (PBPs). However, in mycobacteria, crosslinks formed by L,D-transpeptidases (LDTs) are highly abundant. To elucidate the role of these unusual cr ...

The gastrointestinal tract has recently come to the forefront of multiple research fields. It is now recognized as a major source of signals modulating food intake, insulin secretion and energy balance. It is also a key player in immunity and, through its ...

Functional requirements may constrain phenotypic diversification or foster it. For insect mouthparts, the quantification of the relationship between shape and function in an evolutionary framework remained largely unexplored. Here, the question of a functi ...

Insect flight is characterised by complex time-dependent flows in response to the unsteady wing movements. Biological fliers exploit the unsteady flow fields to modulate aerodynamic forces, thereby displaying unmatched flight performance, especially in hov ...

The High Five (H5) cell line, derived from the lepidopteran Trichoplusia ni, is one of the major insect cell hosts for the production of recombinant proteins using the baculovirus expression vector system (BEVS). Here, we describe a simple polyethylenimine ...

Trypanosomatid parasites are significant causes of human disease and are ubiquitous in insects. Despite the importance of Drosophila melanogaster as a model of infection and immunity and a long awareness that trypanosomatid infection is common in the genus ...

The goal of this thesis was to evaluate the use of artificial transposon systems for the generation of recombinant cell pools and cell lines with Chinese hamster ovary (CHO) cells as the production host. Transposons are naturally occurring genetic elements ...

Due to the high demand for heterogeneous protein production in insect cells, we have established efficient methods for plasmid-based transient gene expression (TGE) in various insect cell lines, including commonly used Spodoptera cell lines Sf9 and HighFiv ...

The aim of the pharmaceutical industry is to manufacture complex therapeutic proteins or biologics to treat human diseases. The manufacture of biologics requires a significant amount of financial and infrastructure resources. For this reason, efforts are b ...

Insect cells have been widely used for the production of recombinant proteins using recombinant baculovirus for gene delivery [1]. To simplify protein production in insect cells, we have previously described a method, based on transient gene expression (TG ...

Helical-Track Bioreactors for Bacterial, Mammalian and Insect Cell Cultures

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Mechanistic Understanding of Neurobehavioral Effects of Insecticides on the Larvae of Zebrafish (Danio rerio)

Rapid molecular evolution of Spiroplasma symbionts of Drosophila

Role of Nimrod receptors in the Drosophila melanogaster cellular immune response

The role of optic flow pooling in insect flight control in cluttered environments

Insect-Inspired Visual Perception for Flight Control and Collision Avoidance

Two Nimrod receptors, NimC1 and Eater, synergistically contribute to bacterial phagocytosis in Drosophila melanogaster

Scalable Production of AAV Vectors in Orbitally Shaken HEK293 Cells

Device and method for sorting biological entities

Bioinspired dual-stiffness origami

The double-edged toxins : how Spiroplasma Ribosome-Inactivating Proteins affect Drosophila melanogaster life history traits

Maturing Mycobacterium smegmatis peptidoglycan requires non-canonical crosslinks to maintain shape

Anatomy and Physiology of the Digestive Tract of Drosophila melanogaster

Form - function relationships in dragonfly mandibles under an evolutionary perspective

Unsteady fluid dynamics around a hovering flat plate wing

A simple plasmid-based transient gene expression method using High Five cells

Infection Dynamics and Immune Response in a Newly Described Drosophila-Trypanosomatid Association

Study of transposon-mediated cell pool and cell line generation in CHO cells

Development of plasmid-based expression technology for rapid and sustained production of recombinant proteins in insect cells

The Engineering of a Scalable Disposable Orbitally Shaken Bioreactor System for Animal Cell Cultivation and Therapeutic Protein Production

Study of the improved Sf9 transient gene expression process