Real-time chemical reaction monitoring with 25 MHz low-field NMR

Real-time reaction monitoring with Nuclear Magnetic Resonance (NMR) spectroscopy is an emerging and increasingly important analytical technique. In this work, we develop and implement software to identify molecules and monitor a chemical reaction in real time using a low-field benchtop NMR operating at 24.35 MHz. The approach was validated using a simple acid-catalyzed elimination reaction, the dehydration of cyclohexanol to cyclohexene, and enabled both qualitative and semi-quantitative assessment of the reaction progress. Automated peak integration and yield calculation were performed at each time step, and the results were benchmarked against a high-field 400 MHz Bruker NMR instrument.