Publications associées à Device and method for sorting biological entities

Design and construction of a microfluidics workstation for high-throughput multi-wavelength fluorescence and transmittance activated droplet analysis and sorting

Christoph Merten, Jatin Panwar, Alexis Louis Autour

Droplet microfluidics has revolutionized quantitative high-throughput bioassays and screening, especially in the field of single-cell analysis where applications include cell characterization, antibody discovery and directed evolution. However, droplet mic ...

NATURE PORTFOLIO2023

Sperm-contributed centrioles segregate stochastically into blastomeres of 4-cell stage Caenorhabditis elegans embryos

Pierre Gönczy, Fernando Romero Balestra

Whereas both sperm and egg contribute nuclear genetic material to the zygote in metazoan organisms, the inheritance of other cellular constituents is unequal between the 2 gametes. Thus, 2 copies of the centriole are contributed solely by the sperm to the ...

GENETICS SOCIETY AMERICA2023

Worldwide analysis of reef surveys sorts coral taxa by associations with recent and past heat stress

Stéphane Joost, Oliver Michele Selmoni, Véronique Berteaux-Lecellier

Coral reefs around the world are under threat from anomalous heat waves that are causing the widespread decline of hard corals. Different coral taxa are known to have different sensitivities to heat, although variation in susceptibilities have also been ob ...

FRONTIERS MEDIA SA2022

Mechanistic Understanding of Neurobehavioral Effects of Insecticides on the Larvae of Zebrafish (Danio rerio)

Sarah-Katharina Könemann

Due to conservation of neuronal functioning across phyla, molecular targets of insecticides are similar in insects and vertebrates. Insecticides thus pose a risk to aquatic vertebrates, such as fish, and potentially cause neurotoxic effects. Although these ...

EPFL2022

Targeted search for scaling genes reveals matrix metalloproteinase 3 as a scaler of the dorsal-ventral pattern in Xenopus laevis embryos

Daria Korotkova

How embryos scale patterning according to size is still not fully understood. Through in silico screening and analysis of reaction-diffusion systems that could be responsible for scaling, we predicted the existence of genes whose expression is sensitive to ...

Cell Press - Elsevier2022

Computational methods for live heart imaging with speed-constrained microscopes

Olivia Mariani

Imaging methods to capture the beating and developing heart inside embryonic animal models such as the zebrafish are a key component for the study of fundamental biological processes such as cardiac birth defects or tissue regeneration. However, live heart ...

EPFL2021

Towards a secure and trustworthy imaging with non-fungible tokens

Touradj Ebrahimi, Evgeniy Upenik, Davi Nachtigall Lazzarotto

Non fungible tokens (NFTs) are used to define the ownership of digital assets. More recently, there has been a surge of platforms to auction digital art as well as other digital assets in form of image, video, and audio content of all sorts. Although NFTs ...

2021

How Many CPU Cores is an FPGA Worth? Lessons Learned from Accelerating String Sorting on a CPU-FPGA System

Paolo Ienne, Mikhail Asiatici, Damian Maiorano

String sorting is a fundamental kernel of string matching and database index construction; yet, it has not been studied as extensively as fixed-length keys sorting. Because processing variable-length keys in hardware is challenging, it is no surprise that ...

SPRINGER2021

From Trees to Barcodes and Back Again: Theoretical and Statistical Perspectives

Kathryn Hess Bellwald, Lida Kanari, Adélie Eliane Garin

Methods of topological data analysis have been successfully applied in a wide range of fields to provide useful summaries of the structure of complex data sets in terms of topological descriptors, such as persistence diagrams. While there are many powerful ...

2020

Three-dimensional electrodes for dielectrophoretic applications

Kevin Keim

This thesis reports the use of metal-coated three-dimensional SU-8 electrodes for dielectrophoretic bio sensing and particle manipulation applications. Placing free standing three-dimensional electrodes in microfluidic channels, electric fields can be appl ...

EPFL2020

Transformation of the Drosophila Sex-Manipulative Endosymbiont Spiroplasma poulsonii and Persisting Hurdles for Functional Genetic Studies

Bruno Lemaitre, Florent François Masson, Chloé Christiane Laurence Jollivet

Insects are frequently infected by bacterial symbionts that greatly affect their physiology and ecology. Most of these endosymbionts are, however, barely tractable outside their native host, rendering functional genetics studies difficult or impossible. Sp ...

AMER SOC MICROBIOLOGY2020

Legacy and alternative halogenated flame retardants in Lake Geneva fish

Benoît Jean Dominique Ferrari

Legacy (i.e., polybrominated diphenyl ethers (PBDEs) and hexabromocyclododecane (HBCDD)) and alternative halogenated flame retardants (HFRs) were analyzed in 31 whole fish samples from Lake Geneva in 2018. Two fish species, namely, the burbot (Lota lota) a ...

2021

Role of Nimrod receptors in the Drosophila melanogaster cellular immune response

Claudia Melcarne

The use of modern molecular biology tools and simple but powerful tractable model organisms such as Drosophila has contributed significantly to our recent advances in the innate immunity field, notably phagocytosis. Since 2001, many phagocytic transmembran ...

EPFL2020

The role of optic flow pooling in insect flight control in cluttered environments

Dario Floreano, Julien Jean Denis Nicolas Lecoeur, Emily Baird

Flight through cluttered environments, such as forests, poses great challenges for animals and machines alike because even small changes in flight path may lead to collisions with nearby obstacles. When flying along narrow corridors, insects use the magnit ...

Nature Research2019

A fish intestinal barrier model to assess the interaction with chemicals in vitro

Hannah Inka Schug

The intestinal epithelium acts as vital gate keeper between the fish and its surrounding environment. A single layer of connected epithelial cells forms a selective barrier, which, among other essential functions, is important for nutrient uptake and defen ...

EPFL2019

Insect-Inspired Visual Perception for Flight Control and Collision Avoidance

Julien Jean Denis Nicolas Lecoeur

Flying robots are increasingly used for tasks such as aerial mapping, fast exploration, video footage and monitoring of buildings. Autonomous flight at low altitude in cluttered and unknown environments is an active research topic because it poses challen ...

EPFL2019

Automated Platform for Long-Term Culture and High-Content Phenotyping of Single C. elegans Worms

Martinus Gijs, Elena Katsyuba, Johan Auwerx, Matteo Cornaglia, Laurent Mouchiroud, Hüseyin Baris Atakan, Rongrong Xiang

The nematode Caenorhabditis elegans is a suitable model organism in drug screening. Traditionally worms are grown on agar plates, posing many challenges for long-term culture and phenotyping of animals under identical conditions. Microfluidics allows for ' ...

Nature Research2019

Efficient Learning from Comparisons

Lucas Maystre

Humans are comparison machines: comparing and choosing an item among a set of alternatives (such as objects or concepts) is arguably one of the most natural ways for us to express our preferences and opinions. In many applications, the analysis of data con ...

EPFL2018

Novel compartment-specific biosensors reveal a complementary subcellular distribution of bioactive Furin and PC7

Pierpaolo Ginefra

Proprotein Convertases (PCs) constitute a family of serine proteases that activate various hormones, growth factors and cell adhesion molecules by mediating endoproteolytic cleavage of their secreted inactive precursors during their transit in the secret ...

EPFL2018

The double-edged toxins : how Spiroplasma Ribosome-Inactivating Proteins affect Drosophila melanogaster life history traits

Mario Gonzalo Garcia Arraez

Many insect species are associated with endosymbiotic bacteria which have the particularity of living within host tissues. Endosymbionts benefit from this stable and nutritious environment, while providing ecological advantages to their host, such as prote ...

EPFL2018