Publications associées à Subwavelength-size local fluorescent sources based on color centers in LiF for scanning near-field optical microscopy

Publications associées (30)

Exploiting Covalent Chemical Labeling with Self-Labeling Proteins

The visualization and manipulation of proteins in live cells are critical for studying complex biological processes. Self-labeling proteins do so by enabling the specific and covalent attachment of synthetic probes, offering unprecedented flexibility in th ...

Annual Reviews2025

Lamellar Schwann Cells in the Pacinian Corpuscle Potentiate Vibration Perception

Graham Knott, Mary-Claude Croisier-Coeytaux, Jérôme Blanc, Stéphanie Clerc

Pacinian corpuscles are among the most sensitive mechanoreceptors found in vertebrates, and they are tuned to vibrations in the highest perceptible frequency range (100 to 2000 Hz). One of their anatomical hallmarks is the onion-like cell layers surroundin ...

AMER ASSOC ADVANCEMENT SCIENCE2025

Bioanode engineering for living solar cells

Charlotte Elisabeth Marie Roullier

Whole-cell biophotovoltaic (BPV) devices leverage photoautotrophic microorganisms like cyanobacteria to convert sunlight and water into bioelectricity. They are capable of growing on sequestered carbon dioxide (CO2), eliminating the need to add exogenous o ...

EPFL2025

Hard x-ray grazing-incidence ptychography: large field-of-view nanostructure imaging with ultra-high surface sensitivity

Manuel Guizar Sicairos

The morphology and distribution of nanoscale structures, such as catalytic active nanoparticles and quantum dots on surfaces, have a significant impact on their function. Thus, the capability of monitoring these properties during manufacturing and operatio ...

Optica Publishing Group2024

Revealing chromatin-specific functions of histone deacylases

Beat Fierz, Carlos Moreno Yruela

Histone deacylases are erasers of Nε-acyl-lysine post-translational modifications and have been targeted for decades for the treatment of cancer, neurodegeneration and other disorders. Due to their relatively promiscuous activity on peptide substrates in v ...

2024

Light- field tomographic fluorescence lifetime imaging microscopy

Edoardo Charbon, Claudio Bruschini, Samuel Burri

Fluorescence lifetime imaging microscopy (FLIM) is a powerful imaging technique that enables the visualization of biological samples at the molecular level by measuring the fluorescence decay rate of fluorescent probes. This provides critical information a ...

National Academy of Sciences2024

Data-Driven Methods for Controller Design in Atomic Force Microscopy

Navid Asmari Saadabad

Ever since the advent of Atomic Force Microscope (AFM) the community of its users is expanding which led to a broad spectrum of applications. Most of these applications require AFM instruments with fast, accurate, and reliable performance. The sensors and ...

EPFL2024

Mitochondrial nanomotion measured by optical microscopy

Sandor Kasas, María Inés Villalba

Nanometric scale size oscillations seem to be a fundamental feature of all living organisms on Earth. Their detection usually requires complex and very sensitive devices. However, some recent studies demonstrated that very simple optical microscopes and de ...

Frontiers Media SA2023

Time-Resolved Scanning Ion Conductance Microscopy and Single-Molecule Spectroscopy

Samuel Mendes Leitão

The evolution of the 3D morphology is at the center of many relevant biological processes ranging from cellular differentiation to cancer invasion and metastasis. Microscopy techniques, such as electron microscopy (EM), super-resolution (SR) optical micros ...

EPFL2023

Nfinder: automatic inference of cell neighborhood in 2D and 3D using nuclear markers

Santiago Nicolás Rodriguez Alvarez

BackgroundIn tissues and organisms, the coordination of neighboring cells is essential to maintain their properties and functions. Therefore, knowing which cells are adjacent is crucial to understand biological processes that involve physical interactions ...

2023

Optical imaging of the small intestine immune compartment across scales

Aleksandra Radenovic, Olivier Burri, Arielle Louise Planchette, Alessio Mylonas

The limitations of 2D microscopy constrain our ability to observe and understand tissue-wide networks that are, by nature, 3-dimensional. Optical projection tomography (OPT) enables the acquisition of large volumes (ranging from micrometres to centimetres) ...

NATURE PORTFOLIO2023

Revealing the Effects of Three Different Antimicrobial Agents on E. coli Biofilms by Using Soft-Probe Scanning Electrochemical Microscopy

Hubert Girault

This paper evaluated the use of soft-probe scanning electrochemical microscopy complementarily with confocal laser scanning microscopy to study the effects of different antimicrobial agents and treatments on E. coli DH5α biofilm. The antimicrobial agents w ...

2023

BigNeuron: a resource to benchmark and predict performance of algorithms for automated tracing of neurons in light microscopy datasets

Pascal Fua, Sean Lewis Hill, Jiancheng Yang, Amos Sironi, Jian Yang

BigNeuron is an open community bench-testing platform with the goal of setting open standards for accurate and fast automatic neuron tracing. We gathered a diverse set of image volumes across several species that is representative of the data obtained in m ...

Nature Publishing Group2023

espm: A Python library for the simulation of STEM-EDXS datasets

Adrien Maxime Louis Teurtrie, Cécile Hébert, Duncan Thomas Lindsay Alexander, Nathanaël Perraudin, Hui Chen, Guillaume Romain Obozinski, Hui Chen

We present two open-source Python packages: "electron spectro-microscopy"(espm) and "electron microscopy tables"(emtables). The espm software enables the simulation of scanning transmission electron microscopy energy-dispersive X-ray spectroscopy datacubes ...

ELSEVIER2023

Bacterial inactivation on sputtered TiOMoN and TiOMoN-Ag thin films under solar simulated light

Lioubov Kiwi, Sami Rtimi

The bacterial inactivation kinetics of Escherichia coli (E. coli) by innovative sputtered TiMoON and TiMoON-Ag films under simulated solar light irradiation is accounted for in a detailed, comprehensive and systematic form. This study presents the evidence ...

ELSEVIER SCIENCE SA2023

Effectiveness of X-ray micro-CT applications upon mafic and ultramafic ophiolitic rocks

Pascal Turberg

X-ray micro-computed tomography (mu CT) was applied upon selected ophiolitic rock samples from various localities of the Vardar ophiolite outcrops in North Greece. Effectiveness of the mu CT application was evaluated through this case study by comparing re ...

2022

Imaging cell biology

Suliana Manley

Imaging technologies drive discovery in cell biology. Innovations in microscopy hardware, imaging methods and computational analysis of large-scale, complex datasets can increase imaging resolution, definition and allow access to new biology. We asked expe ...

NATURE PORTFOLIO2022

Magnetic force microscopy contrast formation and field sensitivity

Marcos Penedo Garcia, Johannes Schwenk

The magnetic force microscope (MFM) is an established experimental tool for imaging stray fields with high spatial resolution and sensitivity. The MFM contrast can however contain contributions from the sample topography, variations in the surface Kelvin p ...

2022

A constrained method for lensless coherent imaging of thin samples

Michaël Unser, Christophe Moser, Manon Rostykus

Lensless inline holography can produce high-resolution images over a large field of view (FoV). In a previous work [Appl. Opt.60, B38 (2021)], we showed that (i) the actual FoV can be extrapolated outside of the camera FoV and (ii) the effective resolution ...

2021

3D Structure From 2D Microscopy Images Using Deep Learning

Suliana Manley, Christian Sieben

Understanding the structure of a protein complex is crucial in determining its function. However, retrieving accurate 3D structures from microscopy images is highly challenging, particularly as many imaging modalities are two-dimensional. Recent advances i ...

Frontiers Media Sa2021