Publications associées à Kinetic and Structural Characterization of the Self-Labeling Protein Tags HaloTag7, SNAP-tag, and CLIP-tag

Publications associées (14)

Ring-Opening Reactions of Aminocyclopropanes and Aminocyclobutanes

Nitrogen-containing compounds are an important class of molecules in medicinal chemistry, chemical biology, biochemistry, material sciences or environmental sciences. Organic nitrogen occurs in many forms, ranging from small building blocks such as urea, a ...

EPFL2022

Nature-Inspired Circular-Economy Recycling for Proteins: Proof of Concept

Francesco Stellacci, Laure Menin, Luc Patiny, Daniel Ortiz Trujillo, Anna Murello, Laura Roset Julia, Shiyu Cheng, Vincenzo Scamarcio, Simone Giaveri

The billion tons of synthetic-polymer-based materials (i.e. plastics) produced yearly are a great challenge for humanity. Nature produces even more natural polymers, yet they are sustainable. Proteins are sequence-defined natural polymers that are constant ...

Wiley2021

Single-Photon, Time-Gated, Phasor-Based Fluorescence Lifetime Imaging through Highly Scattering Medium

Edoardo Charbon, Claudio Bruschini, Arin Can Ülkü

Fluorescence lifetime imaging (FLI) is increasingly recognized as a powerful tool for biochemical and cellular investigations, including in vivo applications. Fluorescence lifetime is an intrinsic characteristic of any fluorescent dye which, to a large ext ...

AMER CHEMICAL SOC2020

Genetically encoded betaxanthin-based small-molecular fluorescent reporter for mammalian cells

Karl Gademann, Leo Scheller

We designed and engineered a dye production cassette encoding a heterologous pathway, including human tyrosine hydroxylase and Amanita muscaria 4,5-DOPA dioxygenase, for the biosynthesis of the betaxanthin family of plant and fungal pigments in mammalian c ...

2020

Nanodiamond Relaxometry-Based Detection of Free-Radical Species When Produced in Chemical Reactions in Biologically Relevant Conditions

Mayeul Sylvain Chipaux

Diamond magnetometry is a quantum sensing method involving detection of magnetic resonances with nanoscale resolution. For instance, T1 relaxation measurements, inspired by equivalent concepts in magnetic resonance imaging (MRI), provide a signal that is e ...

AMER CHEMICAL SOC2020

Small-Molecule Fluorescent Probes for Live-Cell Super-Resolution Microscopy

Kai Johnsson, Lu Wang, Michelle Susanne Frei, Aleksandar Salim

Super-resolution fluorescence microscopy is a powerful tool to visualize biomolecules and cellular structures at the nanometer scale. Employing these techniques in living cells has opened up the possibility to study dynamic processes with unprecedented spa ...

AMER CHEMICAL SOC2019

In Silico Brain Imaging Physically-plausible Methods for Visualizing Neocortical Microcircuitry

Marwan Muhammad Ahmed Abdellah

The recent years have witnessed growing interest in the power of scientific visualization for simulation-based neuroscience. The research presented in this thesis develops methods to generate physically-realistic visualizations of neocortical models recons ...

EPFL2017

Physically-based in silico light sheet microscopy for visualizing fluorescent brain models

Henry Markram, Felix Schürmann, Marwan Muhammad Ahmed Abdellah, Stefan Eilemann, Ahmet Bilgili

We present a physically-based computational model of the light sheet fluorescence microscope (LSFM). Based on Monte Carlo ray tracing and geometric optics, our method simulates the operational aspects and image formation process of the LSFM. This simulated ...

BioMed Central2015

Live-cell multiplane three-dimensional super-resolution optical fluctuation imaging

Theo Lasser, Françoise Gisou van der Goot Grunberg, Marcel Leutenegger, Noelia Laura Bocchio, Aurélien Godinat, Patrick Alain Sandoz, Azat Sharipov

Super-resolution optical fluctuation imaging (SOFI) provides an elegant way of overcoming the diffraction limit in all three spatial dimensions by computing higher-order cumulants of image sequences of blinking fluorophores acquired with a classical widefi ...

Nature Research2014

Advanced Image Processing for Biology, and the Open Bio Image Alliance (OBIA)

Michaël Unser, Daniel Sage, Ricard Delgado Gonzalo

The field of biological imaging has evolved considerably during the past decade as a result of recent (r)evolutions in fluorescence labeling and optical microscopy. Bioimage informatics has been identified as a top priority to cope with the ever-increasing ...

EUSIPCO2013

Interplay between cytoskeletal forces, membrane tension and shape in rapidly migrating cells

Chiara Gabella

Cell migration is a fundamental process in all animal cells. The ability of cell to migrate is crucial for many pathological and physiological conditions such as morphogenesis, the immune response to inflammatory or vascular diseases and cancer metastasis. ...

EPFL2013

Quantitative fluorescence loss in photobleaching for analysis of protein transport and aggregation

Daniel Sage

Background: Fluorescence loss in photobleaching (FLIP) is a widely used imaging technique, which provides information about protein dynamics in various cellular regions. In FLIP, a small cellular region is repeatedly illuminated by an intense laser pulse, ...

Biomed Central Ltd2012

Fluorescence Behavioral Imaging (FBI) tracks identity in heterogeneous groups of Drosophila

Dario Floreano, Pavan P Ramdya, Thomas Schaffter

Distinguishing subpopulations in group behavioral experiments can reveal the impact of differences in genetic, pharmacological and life-histories on social interactions and decision-making. Here we describe Fluorescence Behavioral Imaging (FBI), a toolkit ...

2012

Fluorescent magnetic bead and cell differentiation/counting using a CMOS SPAD matrix

Edoardo Charbon, Martinus Adela Maria Gijs, Caroline Vandevyver, Ulrike Lehmann, Emile Dupont

We present a monolithic silicon chip comprising a matrix of 84 single photon avalanche diodes (SPADs) to detect and discriminate fluorescent beads or fluorescently labeled single cells in a polydimethyl(-siloxane) (PDMS) cartridge that is positioned on top ...

Elsevier2012