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Subcellular Chemical Imaging: New Avenues in Cell Biology

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Publications associées (43)

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Controlled Formation of Nanoribbons and Their Heterostructures via Assembly of Mass-Selected Inorganic Ions

Klaus Kern

Control of nanomaterial dimensions with atomic precision through synthetic methods is essential to understanding and engineering of nanomaterials. For single-layer inorganic materials, size and shape controls have been achieved by self-assembly and surface ...

Wiley-V C H Verlag Gmbh2024

Polarization-sensitive optics diffraction tomography

Demetri Psaltis, Joowon Lim, Amirhossein Saba Shirvan

Polarization of light has been widely used as a contrast mechanism in two-dimensional (2D) microscopy and also in some three-dimensional (3D) imaging modalities. In this paper, we report the 3D tomographic reconstruction of the refractive index (RI) tensor ...

2021

Rapid Untargeted Puff-by-Puff Analysis of (Electronic) Cigarette Emissions by Concentric Dielectric Barrier Discharge Ionisation Mass Spectrometry

Natalia Gasilova, Laure Menin, Luc Patiny, Maria Pavlou, Yong-Qi Gao

Despite the soaring popularity of e-cigarettes among teenagers and young adults, our understanding of the full extent of their health hazards have remained limited. This is due to the vast complexities of e-cigarette aerosols and the difficulty in their fu ...

2024

Composition-tunable transition metal dichalcogenide nanosheets via a scalable, solution-processable method

Kevin Sivula, Andras Kis, Victor Carle Adrien Boureau, Zhenyu Wang, Yanfei Zhao, Simon Nussbaum, Rebekah Anne Wells, Marina Caroline Michèle Caretti, Nicolas Johannes Diercks

The alloying of two-dimensional (2D) transition metal dichalcogenides (TMDs) is an established route to produce robust semiconductors with continuously tunable optoelectronic properties. However, typically reported methods for fabricating alloyed 2D TMD na ...

Royal Soc Chemistry2024

Megapixel SPAD cameras for time-resolved applications

Kazuhiro Morimoto

Progress in optics technology has enriched our daily lives in many ways. Various types of single-photon detector technologies have emerged in the last century. Among these, single-photon avalanche diode (SPAD) technology outperforms other detectors for its ...

EPFL2020

Sequential CNN-based Enhancement of Ultrafast Ultrasound Imaging for Sparse Arrays

Jean-Philippe Thiran, Roser Vinals Terres

With the emergence of ultra-portable ultrasound systems, the need to reduce cost and complexity of ultrasound systems has intensified. Sparse arrays, employing fewer transducer elements to acquire images, offer a viable solution by effectively minimizing d ...

European Signal Processing Conference, EUSIPCO2024

Method and system for generating a three-dimensional model based on spherical photogrammetry

Frédéric Kaplan, Pierre-André Mudry, Nils Hamel, Albane Bénédicte Descombes

A computer-implemented method is proposed for creating a three-dimensional model of the environment. The method comprises the steps of planning (105) a trajectory for a moving system carrying an omnidirectional camera comprising a first image sensor facing ...

2021

Quasi-operando Transmission Electron Microscopy Diagnostics for Electrocatalytic Processes in Liquids

Vasiliki Tileli

It is of great interest to the energy community to understand how the mechano-physico-chemical phenomena that eventually lead to device degradation are related to the startup, operation, and shutdown phases. For electrocatalytic systems operating in liquid ...

Swiss Chemical Soc2024

Systematic Tuning of Rhodamine Spirocyclization for Super-resolution Microscopy

Kai Johnsson, Lu Wang

Rhodamines are the most important class of fluorophores for applications in live-cell fluorescence microscopy. This is mainly because rhodamines exist in a dynamic equilibrium between a fluorescent zwitterion and a nonfluorescent but cell-permeable spirocy ...

AMER CHEMICAL SOC2021

Quantified interface chemistry and biophysics via water imaging

Sylvie Roke

High throughput wide-field second harmonic imaging enables the label-free imaging of interfacial (< 3 nm thick) water, with a spatial resolution of similar to 370 nm using similar to 100 ms acquisition times per image. The obtained interfacial orientationa ...

Spie-Int Soc Optical Engineering2024

3DPD: A photogrammetric pipeline for a PUSH frame stereo cameras

Nicolas Le Thomas

An innovative photogrammetric pipeline has been developed by INAF-Padova for the processing of the stereo images from the CaSSIS (Colour and Stereo Imaging System) (Thomas et al., 2014). CaSSIS is the multispectral stereo push frame camera on board ExoMars ...

PERGAMON-ELSEVIER SCIENCE LTD2021

Single-molecule localization microscopy

Suliana Manley, Juliette Griffie

Single-molecule localization microscopy (SMLM) describes a family of powerful imaging techniques that dramatically improve spatial resolution over standard, diffraction-limited microscopy techniques and can image biological structures at the molecular scal ...

SPRINGERNATURE2021

Surpassing light inhomogeneities in structured-illumination microscopy with FlexSIM

Michaël Unser, Daniel Sage

Super-resolution structured-illumination microscopy (SIM) is a powerful technique that allows one to surpass the diffraction limit by up to a factor two. Yet, its practical use is hampered by its sensitivity to imaging conditions which makes it prone to re ...

2024

Live-cell imaging powered by computation

Suliana Manley

The proliferation of microscopy methods for live-cell imaging offers many new possibilities for users but can also be challenging to navigate. The prevailing challenge in live-cell fluorescence microscopy is capturing intra-cellular dynamics while preservi ...

Nature Portfolio2024

Computational methods for live heart imaging with speed-constrained microscopes

Olivia Mariani

Imaging methods to capture the beating and developing heart inside embryonic animal models such as the zebrafish are a key component for the study of fundamental biological processes such as cardiac birth defects or tissue regeneration. However, live heart ...

EPFL2021

Towards video-rate compressive spontaneous Raman imaging using single-photon avalanche diode arrays

Edoardo Charbon, Claudio Bruschini, Samuel Burri

Compressive Raman imaging has emerged as a promising technique to speed up chemical imaging by compressing the data during acquisition. Yet, current scanning imaging speed is fundamentally limited by the sensors pixel dwell times of at best 1µs. Here, we i ...

SPIE2024

System and methods for differential imaging using a lock-in camera

Christophe Moser, Damien Claude-Marie Loterie, Timothé Laforest, Chiara Bonati

The present invention describes an imaging system that allows visualization of a wide range of samples both in terms of morphology and in terms of material (e.g. density distribution, varying chemical composition, or anything that induces a change of optic ...

2021

Comparing various AI approaches to traditional quantitative assessment of the myocardial perfusion in [82Rb] PET for MACE prediction

Julien René Fageot, Adrien Raphaël Depeursinge

Assessing the individual risk of Major Adverse Cardiac Events (MACE) is of major importance as cardiovascular diseases remain the leading cause of death worldwide. Quantitative Myocardial Perfusion Imaging (MPI) parameters such as stress Myocardial Blood F ...

Nature Portfolio2024

Development of an 11-channel multi wavelength imaging diagnostic for divertor plasmas in MAST Upgrade

Basil Duval, Wouter Vijvers, Yanis Andrebe, Artur Perek

Divertor detachment and alternative divertor magnetic geometries are predicted to be promising approaches to handle the power exhaust of future fusion devices. In order to understand the detachment process caused by volumetric losses in alternative diverto ...

2021

Structure-function analysis of the cyclic β-1,2-glucan synthase from Agrobacterium tumefaciens

Henning Paul-Julius Stahlberg, Dongchun Ni

The synthesis of complex sugars is a key aspect of microbial biology. Cyclic beta-1,2-glucan (C beta G) is a circular polysaccharide critical for host interactions of many bacteria, including major pathogens of humans (Brucella) and plants (Agrobacterium). ...

Nature Portfolio2024