Light-field tomographic fluorescence lifetime imaging microscopy (LIFT-FLIM)

Fluorescence lifetime imaging microscopy (FLIM) measures fluorescence lifetimes of fluorescent probes to investigate molecular interactions. However, conventional FLIM systems often requires extensive scanning that is time-consuming. To address this challenge, we developed a novel computational imaging technique called light field tomographic FLIM (LIFT-FLIM). Our approach acquires volumetric fluorescence lifetime images in a highly data-efficient manner, significantly reducing the number of scanning steps. We demonstrated LIFT-FLIM using a single-photon avalanche diode array on various biological systems. Additionally, we expanded to spectral FLIM and demonstrated high-content multiplexed imaging of lung organoids. LIFT-FLIM can open new avenues in the biomedical research.