Publication

Light-field tomographic fluorescence lifetime imaging microscopy (LIFT-FLIM)

Claudio Bruschini
2024

Abstract

Fluorescence lifetime imaging microscopy (FLIM) measures fluorescence lifetimes of fluorescent probes to investigate molecular interactions. However, conventional FLIM systems often requires extensive scanning that is time-consuming. To address this challenge, we developed a novel computational imaging technique called light field tomographic FLIM (LIFT-FLIM). Our approach acquires volumetric fluorescence lifetime images in a highly data-efficient manner, significantly reducing the number of scanning steps. We demonstrated LIFT-FLIM using a single-photon avalanche diode array on various biological systems. Additionally, we expanded to spectral FLIM and demonstrated high-content multiplexed imaging of lung organoids. LIFT-FLIM can open new avenues in the biomedical research.

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https://infoscience.epfl.ch/entities/publication/2b3f2946-1b31-4cd5-8acf-3e190c3680b6

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Claudio Bruschini
2024

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/2b3f2946-1b31-4cd5-8acf-3e190c3680b6

About this result

Related concepts (32)

Fluorescence in the life sciences

Fluorescence is used in the life sciences generally as a non-destructive way of tracking or analysing biological molecules. Some proteins or small molecules in cells are naturally fluorescent, which is called intrinsic fluorescence or autofluorescence (such as NADH, tryptophan or endogenous chlorophyll, phycoerythrin or green fluorescent protein). Alternatively, specific or general proteins, nucleic acids, lipids or small molecules can be "labelled" with an extrinsic fluorophore, a fluorescent dye which can be a small molecule, protein or quantum dot.

Fluorescence

Fluorescence is the emission of light by a substance that has absorbed light or other electromagnetic radiation. It is a form of luminescence. In most cases, the emitted light has a longer wavelength, and therefore a lower photon energy, than the absorbed radiation. A perceptible example of fluorescence occurs when the absorbed radiation is in the ultraviolet region of the electromagnetic spectrum (invisible to the human eye), while the emitted light is in the visible region; this gives the fluorescent substance a distinct color that can only be seen when the substance has been exposed to UV light.

Förster resonance energy transfer

Förster resonance energy transfer (FRET), fluorescence resonance energy transfer, resonance energy transfer (RET) or electronic energy transfer (EET) is a mechanism describing energy transfer between two light-sensitive molecules (chromophores). A donor chromophore, initially in its electronic excited state, may transfer energy to an acceptor chromophore through nonradiative dipole–dipole coupling. The efficiency of this energy transfer is inversely proportional to the sixth power of the distance between donor and acceptor, making FRET extremely sensitive to small changes in distance.

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Light-field tomographic fluorescence lifetime imaging microscopy (LIFT-FLIM)

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