Publications associées à Biosensor Microprobe Functionalisation Using an On-Chip Dispensing Approach

Development of new volcano-shaped microelectrodes for single cell electrochemistry, electrophysiology and impedance studies

Micro- nano-electrodes have demonstrated superior performances in measuring attenuated intracellular action potentials from electrogenic cell cultures compared to traditional multi-electrode arrays. Yet, the understanding of the critical electrode features ...

EPFL2023

A universal workflow for creation, validation and generalization of detailed neuronal models

Henry Markram, Srikanth Ramaswamy, Alexis Arnaudon, Werner Alfons Hilda Van Geit, Christian Andreas Rössert, Mustafa Anil Tuncel, Tanguy Pierre Louis Damart, Maria Reva, Darshan Mandge

Detailed single neuron modeling is widely used to study neuronal functions. While cellular and functional diversity across the mammalian cortex is vast, most of the available computational tools are dedicated to the reproduction of a small set of specific ...

Cambridge2023

Intracellular recording of cardiomyocyte action potentials with nanopatterned volcano-shaped microelectrodes

Benoît Xavier Emmanuel Desbiolles

Micro-nanotechnology based multielectrode arrays have led to remarkable progress in the field of transmembrane voltage recordings of excitable cells. However, electrode geometries alone have failed to produce a cell-electrode interface that is sufficiently ...

EPFL2020

GABA-mediated tonic inhibition differentially modulates gain in functional subtypes of cortical interneurons

Sean Lewis Hill, Christian Andreas Rössert, Bas-Jan Zandt, Steven Petrou

The binding of GABA (gamma-aminobutyric acid) to extrasynaptic GABA(A) receptors generates tonic inhibition that acts as a powerful modulator of cortical network activity. Despite GABA being present throughout the extracellular space of the brain, previous ...

National Academy of Sciences2020

Data driven building of realistic neuron model using IBEA and CMA evolution strategies

Henry Markram, Werner Alfons Hilda Van Geit, Tanguy Pierre Louis Damart

Recently the building of large neuronal circuits from realistic neuron models has gained traction. This bottom-up approach relies on the accurate description of the primitive elements composing the brain such as neurons and astrocytes, that are then aggreg ...

ACM2020

Intracellular Recording of Cardiomyocyte Action Potentials with Nanopatterned Volcano-Shaped Microelectrode Arrays

Philippe Renaud, Arnaud Bertsch, Benoît Xavier Emmanuel Desbiolles

Micronanotechnology-based multielectrode ar- rays have led to remarkable progress in the field of transmembrane voltage recording of excitable cells. However, providing long-term optoporation- or electroporation-free intracellular access remains a consider ...

American Chemical Society (ACS)2019

Microchannel-based technologies for nerve interfacing

Sandra Gribi

Biomedical technologies in neuroprosthetics are made to restore or replace functions from the nervous system, as well as alleviate pathological conditions. Peripheral nerve interfaces use neuromodulatory techniques to inhibit pain or depression, prevent ep ...

EPFL2019

Characterization of multi-channel intraneural stimulation in transradial amputees

Pablo Garcia del Valle, Edoardo D'Anna, Silvestro Micera, Stanisa Raspopovic, Francesco Maria Petrini, Ivo Strauss, Giacomo Valle, Fiorenzo Artoni

Although peripheral nerve stimulation using intraneural electrodes has been shown to be an effective and reliable solution to restore sensory feedback after hand loss, there have been no reports on the characterization of multi-channel stimulation. A deepe ...

Springer Nature2019

Nonlinear microscopy for temporally and spatially resolved label-free imaging of living cells

Marie Eve Pascale Didier

The human brain consists of approximately a hundred billion of neurons that communicate through a unique series of biochemical and electrical processes. When neuronal networks are solicited, electrochemical processes happen involving millions of cells with ...

EPFL2018

Diamond microelectrode arrays for in vitro neuronal recordings

Michele Giugliano

A novel microfabrication technique for microelectrode arrays (MEAs) with a full diamond-cell interface is demonstrated. Boron-doped nano-crystalline diamond (BNCD) is used as a conductive electrode material on metal tracks insulated by intrinsic NCD. MEAs ...

Cambridge Univ Press2017

Flexible Optoelectric Neural Interface Integrated Wire-Bonding mu LEDs and Microelectrocorticography for Optogenetics

Xiaolin Wang, Xiaowei Gu, Xiaoyang Kang

As an advanced brain-computer interface, the flexible surface electrodearrayhas been used for spatiotemporal localization of neural interactions by recording electrocorticography (ECoG) signals over brain cortical areas. Compared with the electrical stimul ...

Ieee-Inst Electrical Electronics Engineers Inc2017

Biomimetic surface patterning for long-term transmembrane access

Philippe Renaud, Jules John Vandersarl

Here we present a planar patch clamp chip based on biomimetic cell membrane fusion. This architecture uses nanometer length-scale surface patterning to replicate the structure and function of membrane proteins, creating a gigaohm seal between the cell and ...

Nature Research2016

Brief wide-field photostimuli evoke and modulate oscillatory reverberating activity in cortical networks

Michele Giugliano

Cell assemblies manipulation by optogenetics is pivotal to advance neuroscience and neuroengineering. In in vivo applications, photostimulation often broadly addresses a population of cells simultaneously, leading to feed-forward and to reverberating respo ...

Nature Research2016

Subunit-selective N-Methyl-D-aspartate (NMDA) Receptor Signaling through Brefeldin A-resistant Arf Guanine Nucleotide Exchange Factors BRAG1 and BRAG2 during Synapse Maturation

Michael Kintscher

The maturation of glutamatergic synapses in the CNS is regulated by NMDA receptors (NMDARs) that gradually change from a GluN2B- to a GluN2A-dominated subunit composition during postnatal development. Here we show that NMDARs control the activity of the sm ...

Amer Soc Biochemistry Molecular Biology Inc2016

Cell-type- and activity-dependent extracellular correlates of intracellular spiking

Henry Markram, Rodrigo de Campos Perin, Constantinos Anastassiou

Despite decades of extracellular action potential (EAP) recordings monitoring brain activity, the biophysical origin and inherent variability of these signals remains enigmatic. We performed whole-cell patch recordings of excitatory and inhibitory neurons ...

2015

Large Volume Imaging of Rodent Brain Anatomy with Emphasis on Selective Plane Illumination Microscopy

Jean Pierre Ghobril

Inside the human brain there are 86 Billion neurons exchanging electrical impulses to allow reaction, homoeostasis and intelligence. Their coarse organization has been described, leaving us with a fair understanding of macrocircuitry. The microcircuitry of ...

EPFL2015

Finger Probe Array for Topography-Tolerant Scanning Electrochemical Microscopy of Extended Samples

Hubert Girault, Gunther Wittstock, Fernando Cortes Salazar, Dmitry Momotenko, Andreas Stephan Lesch

Scanning electrochemical microscopy with soft microelectrode array probes has recently been used to enable reactivity imaging of extended areas and to compensate sample corrugation perpendicular to the scanning direction. Here, the use of a new type of mic ...

Amer Chemical Soc2014

Command-line cellular electrophysiology for conventional and real-time closed-loop experiments

Michele Giugliano

Background: Current software tools for electrophysiological experiments are limited in flexibility and rarely offer adequate support for advanced techniques such as dynamic clamp and hybrid experiments, which are therefore limited to laboratories with a si ...

Elsevier Science Bv2014

Single-cell RT-PCR, a technique to decipher the electrical, anatomical, and genetic determinants of neuronal diversity

Henry Markram

The patch-clamp technique has allowed for detailed studies on the electrical properties of neurons. Dye loading through patch pipettes enabled characterizing the morphological properties of the neurons. In addition, the patch-clamp technique also allows fo ...

Springer2014

A computer-assisted multi-electrode patch-clamp system

Henry Markram, Rodrigo de Campos Perin

The patch-clamp technique is today the most well-established method for recording electrical activity from individual neurons or their subcellular compartments. Nevertheless, achieving stable recordings, even from individual cells, remains a time-consuming ...

Journal of Visualized Experiments2013