Publications associées à Development of Sample-Adaptable Holders for Lightsheet Microscopy

Design and construction of a microfluidics workstation for high-throughput multi-wavelength fluorescence and transmittance activated droplet analysis and sorting

Christoph Merten, Jatin Panwar, Alexis Louis Autour

Droplet microfluidics has revolutionized quantitative high-throughput bioassays and screening, especially in the field of single-cell analysis where applications include cell characterization, antibody discovery and directed evolution. However, droplet mic ...

NATURE PORTFOLIO2023

Exploring the foundations of tonality: statistical cognitive modeling of modes in the history of Western classical music

Martin Alois Rohrmeier, Daniel Harasim, Fabian Claude Moss

Tonality is one of the most central theoretical concepts for the analysis of Western classical music. This study presents a novel approach for the study of its historical development, exploring in particular the concept of mode. Based on a large dataset of ...

2021

Concordia: A musical XR instrument for playing the solar system

Petter Harald Ericson

Kepler Concordia, a new scientific and musical instrument enabling players to explore the solar system and other data within immersive extended-reality (XR) platforms, is being designed by a diverse team of musicians, artists, scientists and engineers usin ...

ROUTLEDGE JOURNALS, TAYLOR & FRANCIS LTD2020

HISTOBREAST, a collection of brightfield microscopy images of Haematoxylin and Eosin stained breast tissue

Tiberiu Totu

Modern histopathology workflows rely on the digitization of histology slides. The quality of the resulting digital representations, in the form of histology slide image mosaics, depends on various specific acquisition conditions and on the image processing ...

2020

Active illumination and computational Methods for Temporal and Spectral Super-Resolution Microscopy

Christian Jaques

Light microscopy is a tool of paramount importance for biologists and has been constantly improved for the past four centuries. Despite many recent developments, microscopy techniques still require improvement, especially to reach better temporal and spect ...

EPFL2020

High-throughput and adaptive super-resolution fluorescence microscopy of organelles

Dora Mahecic

Fluorescence super-resolution microscopy has allowed unprecedented insight into the workings of biological systems below the diffraction limit of light. Over the past decade, it has overcome several challenges to deliver 3D, multi-color and faster imaging ...

EPFL2020

Dual-Activatable Cell Tracker for Controlled and Prolonged Single-Cell Labeling

Pablo Rivera Fuentes, Salome Riccarda Püntener

Cell trackers are fluorescent chemical tools that facilitate imaging and tracking cells within live organisms. Despite their versatility, these dyes lack specificity, tend to leak outside of the cell, and stain neighboring cells. Here, we report a dual-act ...

AMER CHEMICAL SOC2020

Nanometric axial resolution of fibronectin assembly units achieved with an efficient reconstruction approach for multi-angle-TIRF microscopy

Sébastien Schaub, Emmanuel Emilien Louis Soubies

High resolution imaging of molecules at the cell-substrate interface is required for understanding key biological processes. Here we propose a complete pipeline for multi-angle total internal reflection fluorescence microscopy (MA-TIRF) going from instrume ...

Nature Research2019

Tokamak plasma edge studies by microwave short-pulse reflectometry and backscattering

Pedro Andres Molina Cabrera

Moving towards an increased understanding of the dynamic processes in the edge of tokamak plasmas requires continuous innovation in diagnostic capabilities, pushing the envelope in both spatial and temporal dimenstions. Plasma density and rotation, and the ...

EPFL2019

Compact lensless digital holography

Manon Rostykus

Microscopy is of high interest for biology since it allows imaging features that are too small to be seen with naked eyes. However, cells are mostly transparent to visible and infrared light which makes it difficult to see with a traditional microscope. To ...

EPFL2018

Influence of DNA Binding Dyes on Bare DNA Structure Studied with Atomic Force Microscopy

Giovanni Dietler, Aleksandre Japaridze, Sylvain Renevey, Aleksandr Benke

Fluorescent dyes are widely used for staining and visualization of DNA in optical microscopy based methods. Even though for some dyes the mechanism of binding is known, how this binding affects DNA remains poorly understood. Here we present a novel experim ...

American Chemical Society2015

Contrast Enhancement in Optical Coherence Microscopy

Christophe Pache

Optical Coherence Microscopy (OCM) is a three-dimensional imaging technique that provides cross-sectional views of the subsurface microstructure of biological tissue, with a high axial and lateral resolution. In OCM, a low time coherence light source is sp ...

EPFL2012

Stiffness Tomography by Atomic Force Microscopy

Charles Roduit, Giovanni Dietler, Sandor Kasas, Serguei Sekatski

The atomic force microscope is a convenient tool to probe living samples at the nanometric scale. Among its numerous capabilities, the instrument can be operated as a namo-indenter to gather information about the mechanical properties of the sample. In thi ...

2009

Smooth muscle Notch1 mediates neointimal formation after vascular injury

Freddy Radtke, Yiming Li

Notch1 regulates binary cell fate determination and is critical for angiogenesis and cardiovascular development. However, the pathophysiological role of Notch1 in the postnatal period is not known. We hypothesize that Notch1 signaling in vascular smooth mu ...

2009

Model-Based 2.5-D Deconvolution for Extended Depth of Field in Brightfield Microscopy

Michaël Unser, François Aguet, Dimitri Nestor Alice Van De Ville

Due to the limited depth of field of brightfield microscopes, it is usually impossible to image thick specimens entirely in focus. By optically sectioning the specimen, the in-focus information at the specimen's surface can be acquired over a range of imag ...

IEEE2008

Single molecule detection on surfaces

Marcel Leutenegger

Monitoring biological relevant reactions on the single molecule level based on fluorescence spectroscopy techniques has become one of the most promising approaches for understanding a variety of phenomena in biophysics, biochemistry and life science. By ap ...

EPFL2007

Thrombosis efficacy induced by photodynamic therapy using different photosensitizers

Bernadette Pegaz-Toquet

Various human pathologies, such as cancers or, notably, exudative age-related macular degeneration (AMD), are characterized by an abnormal vascular proliferation (angiogenesis), which in the case of this type of AMD, can rapidly lead to blindness if no cou ...

EPFL2007

Joint Texture and Topography Estimation for Extended Depth of Field in Brightfield Microscopy

Michaël Unser, François Aguet, Dimitri Nestor Alice Van De Ville

Brightfield microscopy often suffers from limited depth of field, which prevents thick specimens from being imaged entirely in-focus. By optically sectioning the specimen, the in-focus regions can be acquired over multiple images. Extended depth of field m ...

IEEE2006

The Colored Revolution of Bioimaging

Michaël Unser, François Aguet, Cédric René Jean Vonesch

With the recent development of fluorescent probes and new high-resolution microscopes, biological imaging has entered a new era and is presently having a profound impact on the way research is being conducted in the life sciences. Biologists have come to d ...

IEEE2006