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Publications associées (31)

Decellularised tissues obtained by a CO2-philic detergent and supercritical CO2

Dominique Pioletti, Pierre-Etienne Bourban, Nathalie Hirt-Burri, Matteo Gregorio Modesto Marascio, Jens Antons, Pierre-Arnaud Aeberhard, Giulia Weissenberger

Tissue decellularisation has gained much attention in regenerative medicine as an alternative to synthetic materials. In decellularised tissues, biological cues can be maintained and provide cellular environments still unmet by synthetic materials. Supercr ...

2018

In vivo pulse labeling of isochronic cohorts of cells in the central nervous system using FlashTag

Subashika Govindan

The tracing of neuronal cell lineages is critical to our understanding of cellular diversity in the CNS. This protocol describes a fluorescence birth-dating technique to label, track and isolate isochronic cohorts of newborn cells in the CNS in vivo in mou ...

NATURE PUBLISHING GROUP2018

Visible spectrum extended-focus optical coherence microscopy for label-free sub-cellular tomography

Theo Lasser, Paul James Marchand, Séverine Coquoz, David Nguyen, Adrien Charles-François Raymond Descloux, Arno Pino Bouwens, Daniel Pawel Szlag, Jérôme Extermann, Miguel Sison

We present a novel extended-focus optical coherence microscope (OCM) attaining 0.7 μm axial and 0.4 μm lateral resolution maintained over a depth of 40 μm, while preserving the advantages of Fourier domain OCM. Our system uses an ultra-broad spectrum from ...

2017

Subsurface ablation of tissue by ultrafast laser

Thomas Jean Victor Marie Lanvin

Laser-induced optical breakdown (LIOB) is a multiphoton process which can be used for selective removal of material. It revolves around the creation of a plasma in the focal volume of a beam, and requires very high peak intensities in the order of the GW.c ...

EPFL2017

Quantitative telomeric chromatin isolation protocol for human cells

Joachim Lingner, Sophie Redon, Jana Majerská

The ends of eukaryotic chromosomes, known as telomeres, consist of repetitive DNA sequences, multiple proteins and noncoding RNAs. Telomeres are dynamic structures that play crucial roles as guardians of genome stability and tumor suppressors. Defects in t ...

2017

Label-free three-dimensional imaging of Caenorhabditis elegans with visible optical coherence microscopy

Theo Lasser, Paul James Marchand, Séverine Coquoz, Johan Auwerx, Arno Pino Bouwens, Laurent Mouchiroud, Daniel Pawel Szlag, Vincenzo Sorrentino

Fast, label-free, high-resolution, three-dimensional imaging platforms are crucial for high-throughput in vivo time-lapse studies of the anatomy of Caenorhabditis elegans, one of the most commonly used model organisms in biomedical research. Despite the ne ...

Public Library of Science2017

Impedance Spectroscopy for Single Cell Analysis and Dispensing

David Vincent Bonzon

Flow cytometry is an essential tool in biology and medicine with applications in immunodeficiency diagnostic, immunosurveillence, organ transplantation in addition to its basic research usage. Before the widespread use of the Fluorescent-Activated Cell Sor ...

EPFL2017

Microfluidic platforms for high-throughput mammalian cell printing, transfection, and dosage-dependent studies

Kristina Pan Woodruff

With the advent of high-throughput and genome-wide screening initiatives, there is a need for improved methods for cell-based assays. Current approaches require expensive equipment, rely on large-scale culturing formats not suited for small or rare sample ...

EPFL2017

Difference in suitable mechanical properties of three-dimensional, synthetic scaffolds for self-renewing mouse embryonic stem cells of different genetic backgrounds

Jeffrey Alan Hubbell, Seung Tae Lee

We evaluated whether the genetic background of embryonic stem cells (ESCs) affects the properties suitable for three-dimensional (3D) synthetic scaffolds for cell self-renewal. Inbred R1 and hybrid B6D2F1 mouse ESC lines were cultured for 7 days in hydroge ...

Wiley2017

Incremental Kinetic Modeling of Spectroscopic Data using a Reduced Calibration Model

Dominique Bonvin, Julien Léo Billeter

The identification of kinetic models is an important step for the monitoring, control and optimization of chemical processes. Kinetic models are often based on first principles that describe the evolution of concentrations by means of conservation and cons ...

2017

Fluorescence lifetime imaging using a single photon avalanche diode array sensor

Claudio Bruschini, Edoardo Charbon, Samuel Burri, Ivan Michel Antolovic

Single photon detectors allows us work with the weakest fluorescence signals. Single photon arrays, combined with ps-controlled gating allow us to create image maps of fluorescence lifetimes, which can be used for in-vivo discrimination of tissue activity. ...

2017

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