Publications de Natalia Gasilova

Publications associées (43)

The pro-apoptotic properties of a phytonutrient rich infusion of A. cherimola leaf extract on AML cells

Natalia Gasilova, Laure Menin, Rita Sarkis

Annonaceae family has broad uses in herbal medicine for treatment of several diseases, whether through seeds' or leaves' extracts. The present study investigates the antiproliferative and antitumor activity of Annona cherimola aqueous leaf (AAL) extract/in ...

2021

The Antioxidant and Proapoptotic Effects of Sternbergia clusiana Bulb Ethanolic Extract on Triple-Negative and Estrogen-Dependent Breast Cancer Cells In Vitro

Natalia Gasilova, Laure Menin

Background: Sternbergia clusiana belongs to the Amaryllidaceae family and is recognized for the valuable biological activity of its major bioactive compounds. The aim of the current is to evaluate the anticancer effects of the ethanolic bulb extract of Ste ...

MDPI2023

Modified cyclodextrins as broad-spectrum antivirals

Matej Janecek, Francesco Stellacci, Natalia Gasilova, Daniel Ortiz Trujillo, Matteo Gasbarri, Valeria Cagno, Samuel Thomas Jones

Viral infections kill millions of people and new antivirals are needed. Nontoxic drugs that irreversibly inhibit viruses (virucidal) are postulated to be ideal. Unfortunately, all virucidal molecules described to date are cytotoxic. We recently developed n ...

AMER ASSOC ADVANCEMENT SCIENCE2020

On-chip mesoporous functionalized magnetic microspheres for protein sequencing by extended bottom-up mass spectrometry

Hubert Girault, Yury Tsybin, Liang Qiao, Natalia Gasilova, Kristina Srzentic

Limited amount and extreme concentration variability of proteomic-related samples require efficient analyte preconcentration and purification prior to the mass spectrometry (MS)-based analysis. Preferably, these steps should be coupled on-line with chosen ...

American Chemical Society2016

From allergen detection to protein-ligand interactions : novel mass spectrometry-based analytical methods

Natalia Gasilova

Fundamental biological and biochemical studies, proteomics, practical nutrition-related and medical applications, all the research fields, where bioanalytical chemistry is an essential tool, constantly require the improvement of existing techniques of bioa ...

EPFL2014

Component-resolved diagnostic of cow’s milk allergy by immunoaffinity capillary electrophoresis – matrix assisted laser desorption/ionization mass spectrometry

Hubert Girault, Natalia Gasilova

Component-resolved diagnostic (CRD) of cow’s milk allergy has been performed using immunoaffinity capillary electrophoresis (IACE) coupled with matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). First, total IgE quantification in the ...

Amer Chemical Soc2014

MALDI Detection of Exosomes: A Potential Tool for Cancer Studies

Hubert Girault, Horst Pick, Natalia Gasilova, Tzu-En Lin, Yingdi Zhu

Matrix-assisted laser desorption ionization time-of-flight mass spectrometry is used to detect intact whole exosomes, yielding exosomal fingerprints within minutes. This rapid exosome detection approach is proposed as a potential tool for cancer studies. M ...

2019

Mass Barcode Signal Amplification for Multiplex Allergy Diagnosis by MALDI-MS

Hubert Girault, Liang Qiao, Natalia Gasilova, Xiaoqin Zhong

A highly sensitive method based on mass-barcoded gold nanoparticles (AuNPs) and immunomagnetic separation has been developed for multiplex allergy diagnosis by MALDI mass spectrometry in a component-resolved manner. Different analytical probes were prepare ...

American Chemical Society2016

Rapid Untargeted Puff-by-Puff Analysis of (Electronic) Cigarette Emissions by Concentric Dielectric Barrier Discharge Ionisation Mass Spectrometry

Natalia Gasilova, Laure Menin, Luc Patiny, Maria Pavlou, Yong-Qi Gao

Despite the soaring popularity of e-cigarettes among teenagers and young adults, our understanding of the full extent of their health hazards have remained limited. This is due to the vast complexities of e-cigarette aerosols and the difficulty in their fu ...

2024

Tyrosine bioconjugation with hypervalent iodine

Jérôme Waser, Natalia Gasilova, Laure Menin, Nina Declas

Hypervalent iodine reagents have recently emerged as powerful tools for late-stage peptide and protein functionalization. Herein we report a tyrosine bioconjugation methodology for the introduction of hypervalent iodine onto biomolecules under physiologica ...

2022

Template directed synthesis of antibody Fc conjugates with concomitant ligand release

Natalia Gasilova

Antibodies are an attractive therapeutic modality for cancer treatment as they allow the increase of the treatment response rate and avoid the severe side effects of chemotherapy. Notwithstanding the strong benefit of antibodies, the efficacy of anti-cance ...

ROYAL SOC CHEMISTRY2022

Site-selective template-directed synthesis of antibody Fc conjugates with concomitant ligand release

Natalia Gasilova, Jean-Manuel Segura

Template-directed methods are emerging as some of the most effective means to conjugate payloads at selective sites of monoclonal antibodies (mAbs). We have previously reported a method based on an engineered Fc-III reactive peptide to conjugate a radionuc ...

Royal Soc Chemistry2023

Structural Analysis of Monoclonal Antibodies with Top-down and Middle-down Electron Transfer Dissociation Mass Spectrometry: The First Decade

Natalia Gasilova, Laure Menin

Monoclonal antibodies (mAbs) are protein biotherapeutics with a proven efficacy toward fighting life-threatening diseases. Their exceptional healing potential drives the annual increase in the number of novel mAbs and other antibody-like molecules entering ...

SWISS CHEMICAL SOC2022

Sensitive and fast identification of bacteria in blood samples by immunoaffinity mass spectrometry for quick BSI diagnosis

Hubert Girault, Horst Pick, Liang Qiao, Natalia Gasilova, Alexandra Bondarenko, Niels Erik Lion, Michel Prudent, Yingdi Zhu

Bloodstream infections rank among the most serious causes of morbidity and mortality in hospitalized patients, partly due to the long period (up to one week) required for clinical diagnosis. In this work, we have developed a sensitive method to quickly and ...

Royal Soc Chemistry2016

Open channel-based microchip electrophoresis interfaced with mass spectrometry via electrostatic spray ionization

Hubert Girault, Liang Qiao, Natalia Gasilova

The coupling between open channel-based microchip electrophoresis and mass spectrometry via electrostatic spray ionization is proposed for in situ detection of fractionated analytes. Electrophoretic separation is performed in an open channel fabricated in ...

Elsevier2016

Characterization of the Time-Domain Isotopic Beat Patterns of Monoclonal Antibodies in Fourier Transform Mass Spectrometry

Natalia Gasilova, Laure Menin

The time-domain transients in the Fourier transform mass spectrometry (FTMS) analysis of monoclonal antibodies (mAbs) are known to exhibit characteristic isotopic beat patterns. These patterns are defined by the isotopic distributions of all gaseous mAb io ...

2022

Highly sensitive detection of five typical fluoroquinolones in low-fat milk by field-enhanced sample injection based CE in bubble cell capillary

Hubert Girault, Liang Qiao, Natalia Gasilova

Fluoroquinolones are a group of synthetic antibiotics with a broad activity spectrum against mycoplasma, gram-positive and gram-negative bacteria. Due to the extensive use of fluoroquinolones in farming and veterinary science, there is a constant need in t ...

Wiley-Blackwell2014

Rapid, Selective Extraction of Trace Amounts of Gold from Complex Water Mixtures with a Metal-Organic Framework (MOF)/Polymer Composite

Emad Oveisi, Wendy Lee Queen, Natalia Gasilova, Shuliang Yang, Daniel Teav Sun

With the ever-increasing production of electronics, there is an ensuing need for gold extraction from sources other than virgin mines. Currently, there are no technologies reported to date that can effectively and selectively concentrate ultratrace amounts ...

AMER CHEMICAL SOC2018

Drug-to-Antibody Ratio Estimation via Proteoform Peak Integration in the Analysis of Antibody-Oligonucleotide Conjugates with Orbitrap Fourier Transform Mass Spectrometry

Yury Tsybin, Natalia Gasilova, Laure Menin, Anton Kozhinov, Konstantin Nagornov

The therapeutic efficacy and pharmacokinetics of antibody-drug conjugates (ADCs) in general, and antibody-oligonucleotide conjugates (AOCs) in particular, depend on the drug-to-antibody ratio (DAR) distribution and average value. The DAR is considered a cr ...

AMER CHEMICAL SOC2021

The methylome of the model arbuscular mycorrhizal fungus, Rhizophagus irregularis, shares characteristics with early diverging fungi and Dikarya

Natalia Gasilova, Laure Menin

Early-diverging fungi (EDF) are distinct from Dikarya and other eukaryotes, exhibiting high N6-methyldeoxyadenine (6mA) contents, rather than 5-methylcytosine (5mC). As plants transitioned to land the EDF sub-phylum, arbuscular mycorrhizal fungi (AMF; Glom ...

NATURE PORTFOLIO2021