Publications de Alberto Schena

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Attempts to create novel ligand-binding proteins often focus on formation of a binding pocket with shape complementarity against the desired ligand (particularly for compounds that lack distinct polar moieties). Although designed proteins often exhibit bin ...

Acetylcholine is a key neurotransmitter, and anticholinesterase agents are essential compounds used as medical drugs, pesticides, and chemical warfare agents. A semisynthetic fluorescence-based probe for the direct, real-time detection of acetylcholine and ...

The development of molecular probes to visualize cellular processes is an important challenge in chemical biology. One possibility to create such cellular indicators is based on the selective labeling of proteins with synthetic probes in living cells. Over ...

Two new water-soluble gold nanoparticles (AO-TEG-Au and AO-PEG-Au NPs) are prepared and characterized. They are stabilized by thioalkylated oligoethylene glycols and functionalized with fluorescent Acridine Orange (AO) derivatives. Despite the different co ...

The invention generally relates to methods, reagents and devices for determining a concentration of an analyte in a sample. Provided is a proteinaceous sensor molecule for detecting an analyte of interest (A), comprising a signal generating moiety tethered ...

We introduce a general method to transform antibodies into ratiometric, bioluminescent sensor proteins for the no-wash quantification of analytes. Our approach is based on the genetic fusion of antibody fragments to NanoLuc luciferase and SNAP-tag, the lat ...

Cell-permeable DNA stains are popular markers in live-cell imaging. Currently used DNA stains for live-cell imaging are either toxic, require illumination with blue light or are not compatible with super-resolution microscopy, thereby limiting their utilit ...

The invention relates to the field of in vitro detection methods using luminescence. Provided is a sensor molecule for detecting an analyte of interest in a sample using bioluminescence resonance energy transfer (BRET), the sensor molecule comprising a pro ...

We introduce luciferases whose emission maxima can be tuned to different wavelengths by chemical labeling. These tunable luciferases are generated by inserting the small luciferase NanoLuc into the structures of the self-labeling proteins SNAP-tag or HaloT ...

Obtaining patient-specific information through the quantification of small molecules and proteins in bodily fluids is essential for personalized therapies. Point-of-care (POC) diagnostic devices hold the promise of delivering such benefit to a wide range o ...

Alberto Schena

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Unintended specificity of an engineered ligand-binding protein facilitated by unpredicted plasticity of the protein fold

Sensing Acetylcholine and Anticholinesterase Compounds

Visualizing Biochemical Activities in Living Cells through Chemistry

Synthesis, characterization, DNA interaction and potential applications of gold nanoparticles functionalized with Acridine Orange fluorophores

Sensor molecules and uses thereof

Bioluminescent Antibodies for Point-of-Care Diagnostics

SiR–Hoechst is a far-red DNA stain for live-cell nanoscopy

Means and methods for bioluminescence resonance energy transfer (bret) analysis in a biological sample

Sensing Acetylcholine and Anticholinesterases and Generalization of Semisynthetic Biosensors

Luciferases with tunable emission wavelengths

Highly Modular Bioluminescent Sensors for Small Molecules and Proteins