Recherche

Afficher tous les résultats pour

Accueil

Personne

Sebastian Maerkl

À propos
Confidentialité
Mentions légales

Graph Chatbot

Publications associées (67)

Page 2 sur 4

Integration column: Microfluidic high-throughput screening

Sebastian Maerkl

Biology has always been a heavily technology limited field. Burgeoning fields such as systems biology require the development and implementation of new technologies, enabling high-throughput and high-fidelity measurements of large systems. Microfluidics pr ...

2009

Targeting protein-ligand neosurfaces with a generalizable deep learning tool

Luc Reymond, Sebastian Maerkl, Philippe Schwaller, Sandrine Madeleine Suzanne Georgeon, Anthony Marchand, Bruno Emanuel Ferreira De Sousa Correia, Pablo Gainza Cirauqui, Leo Scheller, Joseph Giuseppe Schmidt, Stephen Michael Buckley, Maddalena Elia, Arne Schneuing, Yangyang Miao, Martin Pacesa, Evgenia Elizarova, Rebecca Manuela Neeser

Molecular recognition events between proteins drive biological processes in living systems1. However, higher levels of mechanistic regulation have emerged, in which protein-protein interactions are conditioned to small molecules2, 3, 4-5. Despite recent ad ...

NATURE PORTFOLIO2025

Rapid Synthesis of Defined Eukaryotic Promoter Libraries

Sebastian Maerkl, Arun Stephen Rajkumar

Current gene synthesis methods allow the generation of long segments of dsDNA. We show that these techniques can be used to create synthetic regulatory elements and describe a method for the creation of completely defined, synthetic variants of the PHOS pr ...

Amer Chemical Soc2012

Does Positive Selection Drive Transcription Factor Binding Site Turnover? A Test with Drosophila Cis-Regulatory Modules

Sebastian Maerkl

Transcription factor binding site(s) (TFBS) gain and loss (i.e., turnover) is a well-documented feature of cis-regulatory module (CRM) evolution, yet little attention has been paid to the evolutionary force(s) driving this turnover process. The predominant ...

Public Library of Science2011

A partially self-regenerating synthetic cell

Sebastian Maerkl, Nadanai Laohakunakorn, Barbora Lavickova

Self-regeneration is a fundamental function of all living systems. Here we demonstrate partial molecular self-regeneration in a synthetic cell. By implementing a minimal transcription-translation system within microfluidic reactors, the system is able to r ...

Nature Research2020

Live mammalian cell arrays

Matthias Lütolf, Sebastian Maerkl, Samy Gobaa, Luis Miguel Fidalgo Rabanera, Kristina Pan Woodruff

High-content assays have the potential to drastically increase throughput in cell biology and drug discovery, but handling and culturing large libraries of cells such as primary tumor or cancer cell lines requires expensive, dedicated robotic equipment. We ...

Nature Publishing Group2013

A Digital-Analog Microfluidic Platform for Patient-Centric Multiplexed Biomarker Diagnostics of Ultralow Volume Samples

Sebastian Maerkl, Francesca Volpetti, Francesco Piraino

Microfluidic diagnostic devices have the potential to transform the practice of medicine. We engineered a multiplexed digital-analog microfluidic platform for the rapid and highly sensitive detection of 3-4 biomarkers in quadruplicate in 16 independent and ...

Amer Chemical Soc2016

Mechanically Induced Trapping of Molecular Interactions and Its Applications

Sebastian Maerkl

Measuring binding affinities and association/dissociation rates of molecular interactions is important for a quantitative understanding of cellular mechanisms. Many low-throughput methods have been developed throughout the years to obtain these parameters. ...

Sage Publications Inc2016

Topology and dynamics of the zebrafish segmentation clock core circuit

Bart Deplancke, Sebastian Maerkl, Alina Isakova, Andrew Charles Oates, Daniele Soroldoni

During vertebrate embryogenesis, the rhythmic and sequential segmentation of the body axis is regulated by an oscillating genetic network termed the segmentation clock. We describe a new dynamic model for the core pace-making circuit of the zebrafish segme ...

2012

Multi-Target Tracking Of Packed Yeast Cells

Michaël Unser, Nicolas Dénervaud, Sebastian Maerkl, Ricard Delgado Gonzalo

The tracking of cell populations in time-lapse microscopy images enables high-throughput spatiotemporal measurements of cell dynamics. In this paper, we present a new algorithm to simultaneously track many cells in crowded areas. The algorithm runs in real ...

Ieee Service Center, 445 Hoes Lane, Po Box 1331, Piscataway, Nj 08855-1331 Usa2010

A high-throughput microfluidic nanoimmunoassay for detecting anti-SARS-CoV-2 antibodies in serum or ultralow-volume blood samples

Grégoire Michielin, Sebastian Maerkl, Zoe Newell Swank, Hon Ming Andrew Yip

Novel technologies are needed to facilitate large-scale detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) specific antibodies in human blood samples. Such technologies are essential to support seroprevalence studies and vaccine clin ...

National Academy of Sciences2021

Long-Term Single Cell Analysis of S. pombe on a Microfluidic Microchemostat Array

Jean-Bernard Nobs, Sebastian Maerkl

Although Schyzosaccharomyces pombe is one of the principal model organisms for studying the cell cycle, surprisingly few methods have characterized S. pombe growth on the single cell level, and no methods exist capable of analyzing thousands of cells and t ...

Public Library of Science2014

p High-Throughput Single-Cell TCR-pMHC Dissociation Rate Measurements Performed by an Autonomous Microfluidic Cellular Processing Unit

Sebastian Maerkl, Fabien Louis Claude Robert Jammes, George Coukos

We developed an integrated microfluidic cellular processing unit (mCPU) capable of autonomously isolating single cells and performing measurements and on-the-fly analysis of cell- surface dissociation rates, followed by recovery of selected cells. We perfo ...

AMER CHEMICAL SOC2022

Bottom-Up Construction of Complex Biomolecular Systems With Cell-Free Synthetic Biology

Grégoire Michielin, Sebastian Maerkl, Zoe Newell Swank, Nadanai Laohakunakorn, Barbora Lavickova, Amir Shahein, Laura Sophie Grasemann

Cell-free systems offer a promising approach to engineer biology since their open nature allows for well-controlled and characterized reaction conditions. In this review, we discuss the history and recent developments in engineering recombinant and crude e ...

2020

A software-programmable microfluidic device for automated biology

Sebastian Maerkl, Luis Miguel Fidalgo Rabanera

Specific-purpose microfluidic devices have had considerable impact on the biological and chemical sciences, yet their use has largely remained limited to specialized laboratories. Here we present a general-purpose software-programmable microfluidic device ...

Royal Society of Chemistry2011

Single-Cell Tracing Dissects Regulation of Maintenance and Inheritance of Transcriptional Reinduction Memory

Sebastian Maerkl, Johannes Becker

Transcriptional memory of gene expression enables adaptation to repeated stimuli across many organisms. However, the regulation and heritability of transcriptional memory in single cells and through divisions remains poorly understood. Here, we combined mi ...

2020

Two distinct promoter architectures centered on dynamic nucleosomes control ribosomal protein gene transcription

Felix Naef, Philippe Jacquet, Nicolas Dénervaud, Sebastian Maerkl, Jacques Rougemont

In yeast, ribosome production is controlled transcriptionally by tight coregulation of the 138 ribosomal protein genes (RPGs). RPG promoters display limited sequence homology, and the molecular basis for their coregulation remains largely unknown. Here we ...

Cold Spring Harbor Laboratory Press2014

A high-throughput nanoimmunoassay chip applied to large-scale vaccine adjuvant screening

Jeffrey Alan Hubbell, Sebastian Maerkl

Large-scale experimentation is becoming instrumental in enabling new discoveries in systems biology and personalized medicine. We developed a multiplexed high-throughput nanoimmunoassay chip capable of quantifying four biomarkers in 384 5 nL samples, for a ...

Royal Soc Chemistry2013

On biochemical constructors and synthetic cells

Sebastian Maerkl

Is it possible to build life? More specifically, is it possible to create a living synthetic cell from inanimate building blocks? This question precipitated into one of the most significant grand challenges in biochemistry and synthetic biology, with sever ...

ROYAL SOC2023

A microfluidic platform for high-throughput multiplexed protein quantitation

Sebastian Maerkl, Francesca Volpetti

We present a high-throughput microfluidic platform capable of quantitating up to 384 biomarkers in 4 distinct samples by immunoassay. The microfluidic device contains 384 unit cells, which can be individually programmed with pairs of capture and detection ...

Public Library of Science2015