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Pierre Gönczy

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Publications associées (110)

Resolution Doubling in 3D-STORM Imaging through Improved Buffers

Pierre Gönczy, Debora Keller, Suliana Manley

Super-resolution imaging methods have revolutionized fluorescence microscopy by revealing the nanoscale organization of labeled proteins. In particular, single-molecule methods such as Stochastic Optical Reconstruction Microscopy (STORM) provide resolution ...

Public Library of Science2013

Analysis of centriole elimination during C. elegans oogenesis

Graham Knott, Pierre Gönczy, Tamara Mikeladze-Dvali, Petr Strnad, Lukas von Tobel

Centrosomes are the principal microtubule organizing centers (MTOCs) of animal cells and comprise a pair of centrioles surrounded by pericentriolar material (PCM). Centriole number must be carefully regulated, notably to ensure bipolar spindle formation an ...

2012

Cortical dynein is critical for proper spindle positioning in human cells

Pierre Gönczy, Coralie Busso, Sachin Kotak

Correct spindle positioning is fundamental for proper cell division during development and in stem cell lineages. Dynein and an evolutionarily conserved ternary complex (nuclear mitotic apparatus protein [NuMA]–LGN–Gα in human cells and LIN-5–GPR-1/2–Gα in ...

Rockefeller University Press2012

Towards a molecular architecture of centriole assembly

Pierre Gönczy

The centriole is an evolutionarily conserved macromolecular structure that is crucial for the formation of flagella, cilia and centrosomes. The ultrastructure of the centriole was first characterized decades ago with the advent of electron microscopy, reve ...

2012

Cartwheel Architecture of Trichonympha Basal Body

Pierre Gönczy, Paul Guichard

American Association for the Advancement of Science2012

Polarity mediates asymmetric trafficking of the G beta heterotrimeric G-protein subunit GPB-1 in C. elegans embryos

Pierre Gönczy, Kalyani Thyagarajan

Asymmetric cell division is an evolutionarily conserved process that gives rise to daughter cells with different fates. In one-cell stage C. elegans embryos, this process is accompanied by asymmetric spindle positioning, which is regulated by anterior-post ...

2011

The SCF-FBXW5 E3-ubiquitin ligase is regulated by PLK4 and targets HsSAS-6 to control centrosome duplication

Pierre Gönczy, Debora Keller

Deregulated centrosome duplication can result in genetic instability and contribute to tumorigenesis(1,2). Here, we show that centrosome duplication is regulated by the activity of an E3-ubiquitin ligase that employs the F-box protein FBXW5 (ref. 3) as its ...

Nature Publishing Group2011

Structural Basis of the 9-Fold Symmetry of Centrioles

Pierre Gönczy, Debora Keller

The centriole, and the related basal body, is an ancient organelle characterized by a universal 9-fold radial symmetry and is critical for generating cilia, flagella, and centrosomes. The mechanisms directing centriole formation are incompletely understood ...

2011

Spindle positioning in human cells relies on proper centriole formation and on the microcephaly proteins CPAP and STIL

Pierre Gönczy, Debora Keller, Gregor Kohlmaier, Petr Strnad, Fernando Romero Balestra

Patients with MCPH (autosomal recessive primary microcephaly) exhibit impaired brain development, presumably due to the compromised function of neuronal progenitors. Seven MCPH loci have been identified, including one that encodes centrosome protein 4.1 as ...

Company of Biologists2011

PP2A Phosphatase Acts upon SAS-5 to Ensure Centriole Formation in C. elegans Embryos

Pierre Gönczy, Debora Keller

Centrosome duplication occurs once per cell cycle and ensures that the two resulting centrosomes assemble a bipolar mitotic spindle. Centriole formation is fundamental for centrosome duplication. In Caenorhabditis elegans, the evolutionarily conserved prot ...

Cell Press - Elsevier2011

Temperature gradient stimulation for cell division in C. Elegans Embryos on chip

Philippe Renaud, Pierre Gönczy, Christian Adamczyk, Alexandra Bezler, Sophie Baranek Delasoie

This paper reports on a new microfluidic device for temperature stimulation of cell in in-vitro culture. Micro- electrodes in a meander shape are embedded into the microfluidic channels to generate either a temperature gradient through the culture chamber ...

2010

Mutual Antagonism Between the Anaphase Promoting Complex and the Spindle Assembly Checkpoint Contributes to Mitotic Timing in Caenorhabditis elegans

Pierre Gönczy, Alexandra Bezler

The anaphase promoting complex/cyclosome (APC/C) triggers the separation of sister chromatids and exit from mitosis across eukaryotic evolution. The APC/C is inhibited by the spindle assembly checkpoint (SAC) until all chromosomes have achieved bipolar att ...

2010

ASSET: A robust algorithm for the automated segmentation and standardization of early Caenorhabditis elegans embryos

Felix Naef, Simon Blanchoud, Pierre Gönczy, Yemima Suryani Budirahardja

The early Caenorhabditis elegans embryo is an attractive model to investigate evolutionarily conserved cellular mechanisms. However, there is a paucity of automated methods to gather quantitative information with subcellular precision in this system. We de ...

Wiley-Blackwell2010

Regulation of cortical contractility and spindle positioning by the protein phosphatase 6 PPH-6 in one-cell stage C. elegans embryos

Pierre Gönczy

Modulation of the microtubule and the actin cytoskeleton is crucial for proper cell division. Protein phosphorylation is known to be an important regulatory mechanism modulating these cytoskeletal networks. By contrast, there is a relative paucity of infor ...

2010

NuMA-related LIN-5, ASPM-1, calmodulin and dynein promote meiotic spindle rotation independently of cortical LIN-5/GPR/Galpha

Pierre Gönczy

The spindle apparatus dictates the plane of cell cleavage, which is critical in the choice between symmetric or asymmetric division. Spindle positioning is controlled by an evolutionarily conserved pathway, which involves LIN-5/GPR-1/2/Galpha in Caenorhabd ...

2009

Overly long centrioles and defective cell division upon excess of the SAS-4-related protein CPAP

Pierre Gönczy, Gregor Kohlmaier

The centrosome is the principal microtubule organizing center (MTOC) of animal cells. Accurate centrosome duplication is fundamental for genome integrity and entails the formation of one procentriole next to each existing centriole, once per cell cycle. Th ...

2009

Phosphorylation of SAS-6 by ZYG-1 is critical for centriole formation in C. elegans embryos

Pierre Gönczy, Coralie Busso, Isabelle Fluckiger

Despite being essential for proper cell division, the mechanisms governing centrosome duplication are incompletely understood and represent an important open question in cell biology. Formation of a new centriole next to each existing one is critical for c ...

Cell Press - Elsevier2009

Coupling the cell cycle to development

Pierre Gönczy, Yemima Suryani Budirahardja

The core machinery that drives the eukaryotic cell cycle has been thoroughly investigated over the course of the past three decades. It is only more recently, however, that light has been shed on the mechanisms by which elements of this core machinery are ...

2009

Mechanisms of asymmetric cell division: flies and worms pave the way

Pierre Gönczy

Asymmetric cell division is fundamental for generating diversity in multicellular organisms. The mechanisms that govern asymmetric cell division are increasingly well understood, owing notably to studies that were conducted in Drosophila melanogaster and C ...

2008

Structural determinants underlying the temperature-sensitive nature of a Galpha mutant in asymmetric cell division of Caenorhabditis elegans

Pierre Gönczy

Heterotrimeric G-proteins are integral to a conserved regulatory module that influences metazoan asymmetric cell division (ACD). In the Caenorhabditis elegans zygote, GOA-1 (Galpha(o)) and GPA-16 (Galpha(i)) are involved in generating forces that pull on a ...

2008

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