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Pierre Gönczy

Cette personne a quitté l’EPFL

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Publications associées (103)

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Correlative multicolor 3D SIM and STORM microscopy

Pierre Gönczy, Arne Seitz, Romain Guiet, Virginie Hamel, Paul Guichard, Mathias Fournier

Within the last decade, super-resolution methods that surpass the diffraction limit of light microscopy have provided invaluable insight into a variety of biological questions. Each of these approaches has inherent advantages and limitations, such that the ...

Optical Soc Amer2014

Extensive programmed centriole elimination unveiled in C. elegans embryos

Pierre Gönczy, Nils Kalbfuss

Centrioles are critical for fundamental cellular processes, including signaling, motility, and division. The extent to which centrioles are present after cell cycle exit in a developing organism is not known. The stereotypical lineage of Caenorhabditis ele ...

2023

Centriolar CPAP/SAS-4 Imparts Slow Processive Microtubule Growth

Ashwani Sharma, Pierre Gönczy, Mary-Claude Croisier-Coeytaux

Centrioles are fundamental and evolutionarily conserved microtubule-based organelles whose assembly is characterized by microtubule growth rates that are orders of magnitude slower than those of cytoplasmic microtubules. Several centriolar proteins can int ...

Cell Press - Elsevier2016

Centrosomes and cancer: revisiting a long-standing relationship

Pierre Gönczy

Over a century ago, centrosome aberrations were postulated to cause cancer by promoting genome instability. The mechanisms governing centrosome assembly and function are increasingly well understood, allowing for a timely reappraisal of this postulate. Thi ...

Nature Publishing Group2015

Discovering Regulators of Centriole Biogenesis through siRNA-Based Functional Genomics in Human Cells

Pierre Gönczy, Fernando Romero Balestra, Petr Strnad

Centrioles are essential for forming cilia, flagella, and centrosomes and are thus critical for a range of fundamental cellular processes. Despite their importance, the mechanisms governing centriole biogenesis remain incompletely understood. We performed ...

Cell Press - Elsevier2013

TRIM37 prevents formation of centriolar protein assemblies by regulating Centrobin

Tessa Vivianne Averink, Pierre Gönczy, Coralie Busso, Fernando Romero Balestra, Benita Wolf

TRIM37 is an E3 ubiquitin ligase mutated in Mulibrey nanism, a disease with impaired organ growth and increased tumor formation. TRIM37 depletion from tissue culture cells results in supernumerary foci bearing the centriolar protein Centrin. Here, we chara ...

2021

Simple buffers for 3D STORM microscopy

Vinoth Edal Joseph Sundar Rajan, Pierre Gönczy, Suliana Manley, Debora Keller

3D STORM is one of the leading methods for super-resolution imaging, with resolution down to 10 nm in the lateral direction, and 30-50 nm in the axial direction. However, there is one important requirement to perform this type of imaging: making dye molecu ...

Optical Soc Amer2013

KAT2A/KAT2B-targeted acetylome reveals a role for PLK4 acetylation in preventing centrosome amplification

Pierre Gönczy, Mathias Fournier

Lysine acetylation is a widespread post-translational modification regulating various biological processes. To characterize cellular functions of the human lysine acetyltransferases KAT2A (GCN5) and KAT2B (PCAF), we determined their acetylome by shotgun pr ...

Nature Research2016

Mechanisms of HsSAS-6 assembly promoting centriole formation in human cells

Pierre Gönczy, Suliana Manley, Romain Wyss, Debora Keller

SAS-6 proteins are thought to impart the ninefold symmetry of centrioles, but the mechanisms by which their assembly occurs within cells remain elusive. In this paper, we provide evidence that the N-terminal, coiled-coil, and C-terminal domains of HsSAS-6 ...

Rockefeller University Press2014

Native Architecture of the Centriole Proximal Region Reveals Features Underlying Its 9-Fold Radial Symmetry

Pierre Gönczy, Davide Demurtas, Paul Guichard, Aitana Neves da Silva

Centrioles are cylindrical microtubule-based structures whose assembly is critical for the formation of cilia, flagella, and centrosomes. The centriole proximal region harbors a cartwheel that dictates the 9-fold symmetry of centrioles. Although the cartwh ...

2013

Structures of SAS-6 coiled coil hold implications for the polarity of the centriolar cartwheel

Pierre Gönczy, Georgios Hatzopoulos

Centrioles are eukaryotic organelles that template the formation of cilia and flagella, as well as organize the microtubule network and the mitotic spindle in animal cells. Centrioles have proximal-distal polarity and a 9 fold radial symmetry imparted by a ...

CELL PRESS2022

Sequential Protein Recruitment in C. elegans Centriole Formation

Pierre Gönczy

Formation of the microtubule-based centriole is a poorly understood process that is crucial for duplication of the centrosome, the principal microtubule-organizing center of animal cells . Five proteins have been identified as being essential for centriole ...

2006

Regulated HsSAS-6 levels ensure formation of a single procentriole per centriole during the centrosome duplication cycle

Pierre Gönczy, Petr Strnad

Centrosome duplication involves the formation of a single procentriole next to each centriole, once per cell cycle. The mechanisms governing procentriole formation and those restricting its occurrence to one event per centriole are poorly understood. Here, ...

Cell Press - Elsevier2007

Stereotyped distribution of midbody remnants in early C. elegans embryos requires cell death genes and is dispensable for development

Pierre Gönczy, Christian Gentili

Nature Publishing Group2014

Pierre Gönczy

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Correlative multicolor 3D SIM and STORM microscopy

Extensive programmed centriole elimination unveiled in C. elegans embryos

Centriolar CPAP/SAS-4 Imparts Slow Processive Microtubule Growth

Centrosomes and cancer: revisiting a long-standing relationship

Discovering Regulators of Centriole Biogenesis through siRNA-Based Functional Genomics in Human Cells

TRIM37 prevents formation of centriolar protein assemblies by regulating Centrobin

Simple buffers for 3D STORM microscopy

KAT2A/KAT2B-targeted acetylome reveals a role for PLK4 acetylation in preventing centrosome amplification

Mechanisms of HsSAS-6 assembly promoting centriole formation in human cells

Native Architecture of the Centriole Proximal Region Reveals Features Underlying Its 9-Fold Radial Symmetry

Structures of SAS-6 coiled coil hold implications for the polarity of the centriolar cartwheel

Sequential Protein Recruitment in C. elegans Centriole Formation

Regulated HsSAS-6 levels ensure formation of a single procentriole per centriole during the centrosome duplication cycle

Multicolor Single-Particle Reconstruction of Protein Complexes

NuMA links the mitotic spindle with plasma membrane lipids

Cortical Dynein Powered by Polarized Actomyosin Contractions and Pronuclear Dynein Separate Centrosomes

Paternally contributed centrioles exhibit exceptional persistence in C-elegans embryos

Cartwheel Architecture of Trichonympha Basal Body

NuMA phosphorylation dictates dynein-dependent spindle positioning

Stereotyped distribution of midbody remnants in early C. elegans embryos requires cell death genes and is dispensable for development