Benjamin Rappaz

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Publications associées (32)

Optical recording of cell electrical activity by Digital Holographic Microscopy

Christian Depeursinge, Pierre Marquet, Pierre Magistretti, Pascal Jourdain, Daniel Boss, Benjamin Rappaz

2009

Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy

Tristan Colomb, Christian Depeursinge, Pierre Marquet, Jonas Kühn, Pierre Magistretti, Benjamin Rappaz, Viesturs Simanis, Elena Cano

Digital holography microscopy (DHM) is an optical technique which provides phase images yielding quantitative information about cell structure and cellular dynamics. Furthermore, the quantitative phase images allow the derivation of other parameters, inclu ...

SPIE Society of Photo-optical Instrumentation Engineers2009

Submicrometer tomography of cells by multiple-wavelength digital holographic microscopy in reflection

Tristan Colomb, Frédéric Montfort, Christian Depeursinge, Pierre Marquet, Jonas Kühn, Nicolas Pavillon, Benjamin Rappaz, Corinne Moratal

We present first results on a method enabling mechanical scanning-free tomography with submicrometer axial resolution by multiple-wavelength digital holographic microscopy. By sequentially acquiring reflection holograms and summing 20 wavefronts equally sp ...

2009

Simultaneous cell morphometry and refractive index measurement with dual- wavelength digital holographic microscopy and dye-enhanced dispersion of perfusion medium

Christian Depeursinge, Pierre Marquet, Florian Charrière, Pierre Magistretti, Benjamin Rappaz

Digital holographic microscopy (DHM) allows optical-path-difference (OPD) measurements with nanometric accuracy. OPD induced by transparent cells depends on both the refractive index (RI) of cells and their morphology. This Letter presents a dual-wavelengt ...

2008

Cellular dynamics explored with digital holographic microscopy

Benjamin Rappaz

Obtaining high resolution 3D quantitative images is critical for elucidating the relationship between cell dynamics and physiological or pathological processes. However the feasibility of such cellular dynamic studies is limited by the resolution of the 3D ...

EPFL2008

Comparative study of human erythrocytes by digital holographic microscopy, confocal microscopy, and impedance volume analyzer

Christian Depeursinge, Pierre Marquet, Pierre Magistretti, Benjamin Rappaz

Red blood cell (RBC) parameters such as morphology, volume, refractive index, and hemoglobin content are of great importance for diagnostic purposes. Existing approaches require complicated calibration procedures and robust cell perturbation. As a result, ...

2008

Influence of shot noise on phase measurement accuracy in digital holographic microscopy

Tristan Colomb, Christian Depeursinge, Pierre Marquet, Jonas Kühn, Florian Charrière, Benjamin Rappaz

Digital Holographic Microscopy (DHM) is a single shot interferometric technique, which provides quantitative phase images with subwavelength axial accuracy. A short hologram acquisition time (down to microseconds), allows DHM to offer a reduced sensitivity ...

2007

Digital holographic microscopy applied to life sciences

Tristan Colomb, Christian Depeursinge, Pierre Marquet, Jonas Kühn, Florian Charrière, Benjamin Rappaz

Digital holographic microscopy (DHM) is applied to life sciences applications and demonstrate its capability of real-time imaging and quantitative measurements of physiological parameters such as cell volume or mean cell hemoglobin concentration (MCHC) of ...

IEEE2007

Living specimen tomography by digital holographic microscopy: morphometry of testate amoeba

Tristan Colomb, Christian Depeursinge, Pierre Marquet, Florian Charrière, Nicolas Pavillon, Edward Mitchell, Benjamin Rappaz, Thierry Jean Heger

This paper presents an optical diffraction tomography technique based on digital holographic microscopy. Quantitative 2-dimensional phase images are acquired for regularly-spaced angular positions of the specimen covering a total angle of π, allowing to bu ...

2006

Measurement of the integral refractive index and dynamic cell morphometry of living cells with digital holographic microscopy

Etienne Cuche, Christian Depeursinge, Pierre Marquet, Pierre Magistretti, Benjamin Rappaz

We have developed a digital holographic microscope (DHM), in a transmission mode, adapted to the quantitative study of cellular dynamics. Living cells in culture are optically probed by measuring the phase shift they produce on the transmitted wave front. ...

2005

Digital holographic microscopy for study cellular dynamics

Etienne Cuche, Christian Depeursinge, Pierre Marquet, Pierre Magistretti, Benjamin Rappaz

Based on an original numerical reconstruction algorithm (E. Cuche et al. Appl. Opt. 38, 6994 1999), we have developed a Digital Holographic Microscope (DHM), in a transmission mode, allowing to investigate noninvasively cellular structures and dynamics. DH ...

SPIE2005

Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy

Tristan Colomb, Etienne Cuche, Christian Depeursinge, Pierre Marquet, Pierre Magistretti, Benjamin Rappaz

We have developed a digital holographic microscope (DHM), in a transmission mode, especially dedicated to the quantitative visualization of phase objects such as living cells. The method is based on an original numerical algorithm presented in detail elsew ...

2005

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