Benjamin Rappaz

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Related publications (32)

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Image- and Fluorescence-Based Test Shows Oxidant-Dependent Damages in Red Blood Cells and Enables Screening of Potential Protective Molecules

Gerardo Turcatti, Benjamin Rappaz, Michel Prudent, Manon Sandra Bardyn

An increase of oxygen saturation within blood bags and metabolic dysregulation occur during storage of red blood cells (RBCs). It leads to the gradual exhaustion of RBC antioxidant protective system and, consequently, to a deleterious state of oxidative st ...

2021

Living specimen tomography by digital holographic microscopy: morphometry of testate amoeba

Pierre Marquet, Christian Depeursinge, Tristan Colomb, Nicolas Pavillon, Benjamin Rappaz, Florian Charrière

This paper presents an optical diffraction tomography technique based on digital holographic microscopy. Quantitative 2-dimensional phase images are acquired for regularly-spaced angular positions of the specimen covering a total angle of π, allowing to bu ...

2006

Label-Free Cytotoxicity Screening Assay by Digital Holographic Microscopy

Pierre Magistretti, Christian Depeursinge, Gerardo Turcatti, Benjamin Rappaz, Jonas Kühn, Marc Chambon, Etienne Shaffer, Jérôme Parent, Julien Mena

We introduce a label-free technology based on digital holographic microscopy (DHM) with applicability for screening by imaging, and we demonstrate its capability for cytotoxicity assessment using mammalian living cells. For this first high content screenin ...

Mary Ann Liebert2013

Digital holographic microscopy applied to life sciences

Pierre Marquet, Christian Depeursinge, Jonas Kühn, Tristan Colomb, Benjamin Rappaz, Florian Charrière

Digital holographic microscopy (DHM) is applied to life sciences applications and demonstrate its capability of real-time imaging and quantitative measurements of physiological parameters such as cell volume or mean cell hemoglobin concentration (MCHC) of ...

IEEE2007

Phase Retrieval by Using Transport-of-Intensity Equation and Differential Interference Contrast Microscopy

Michaël Unser, Daniel Sage, Benjamin Rappaz, Emrah Bostan, Emmanuel Augustin Julien Froustey

We present a variational reconstruction algorithm for the phase-retrieval problem by using the differential interference contrast microscopy. Principally, we rely on the transport-of-intensity equation that specifies the sought phase as the solution of a p ...

IEEE2014

Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy

Pierre Magistretti, Pierre Marquet, Christian Depeursinge, Tristan Colomb, Benjamin Rappaz, Etienne Cuche

We have developed a digital holographic microscope (DHM), in a transmission mode, especially dedicated to the quantitative visualization of phase objects such as living cells. The method is based on an original numerical algorithm presented in detail elsew ...

2005

Measurement of the integral refractive index and dynamic cell morphometry of living cells with digital holographic microscopy

Pierre Magistretti, Pierre Marquet, Christian Depeursinge, Benjamin Rappaz, Etienne Cuche

We have developed a digital holographic microscope (DHM), in a transmission mode, adapted to the quantitative study of cellular dynamics. Living cells in culture are optically probed by measuring the phase shift they produce on the transmitted wave front. ...

2005

Automated fast label-free quantification of cardiomyocyte dynamics with raw holograms for cardiotoxicity screening

Gerardo Turcatti, Benjamin Rappaz

Traditional cell analysis approaches based on quantitative phase imaging (QPI) necessitate a reconstruction stage, which utilizes digital holography. However, phase retrieval processing can be complicated and time-consuming since it needs numerical reconst ...

Optica Publishing Group2025

Loss of Protein Tyr-phosphorylation During in vitro Storage of Human Erythrocytes: Impact on RBC Morphology

Pierre Marquet, Gerardo Turcatti, Niels Erik Lion, Benjamin Rappaz, Michel Prudent

Wiley-Blackwell2014

Digital holographic microscopy for study cellular dynamics

Pierre Magistretti, Pierre Marquet, Christian Depeursinge, Benjamin Rappaz, Etienne Cuche

Based on an original numerical reconstruction algorithm (E. Cuche et al. Appl. Opt. 38, 6994 1999), we have developed a Digital Holographic Microscope (DHM), in a transmission mode, allowing to investigate noninvasively cellular structures and dynamics. DH ...

SPIE2005

Red blood cell structure and dynamics explored with digital holographic microscopy

Pierre Magistretti, Pierre Marquet, Christian Depeursinge, Benjamin Rappaz, Pascal Jourdain, Daniel Boss

2009

Optical recording of cell electrical activity by Digital Holographic Microscopy

Pierre Magistretti, Pierre Marquet, Christian Depeursinge, Benjamin Rappaz, Daniel Boss, Pascal Jourdain

2009