Publications de Kristopher Pataky

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This thesis work focuses on applying microtechnology to produce three toolkits for life-sciences research. The first technique presented is nanostencil lithography for patterning cell adhesions. Nanostencil lithography is a shadow-mask micro and nanopatter ...

We provide a common theoretical framework reuniting specific models for the Ca2+-alginate system and general reaction diffusion theory along with experimental validation on a microfluidic chip. As a starting point, we use a set of nonlinear, partial differ ...

NMR spectra of mammalian cells are taken using surface microprobes that are based on microfabricated planar coils. The surface microprobe resembles a miniaturized Petri dish commonly used in biological research. The diameter of the planar coils is 1 mm. Ch ...

Controlling alginate gel formation by diffusion of Ca2+ ions through a filter barrier, a layer-by-layer deposition technique with resolution on the size scale of a single cell is presented. It offers the possibility of exposing cells under biocompatible co ...

53BP1, the vertebrate ortholog of the budding yeast Rad9 and fission yeast Crb2/Rhp9 checkpoint proteins, is recruited rapidly to sites of DNA double-strand breaks (DSBs). A tandem tudor domain in human 53BP1 that recognizes methylated residues in the hist ...

Vascularization arguably poses the most significant hurdle for the success of most biomaterials-based tissue engineering therapies. In this work, we report two printing strategies that permit the 3D Inkjet printing of fluorescent alginate hydro gels into o ...

Resistless processes to realize the pattern transfer of your designs into the substrate present some advantages, as the reduction in fabrication steps. Stencil Lithography (SL) is one of the most used resistless processes and, up to now, it has mainly been ...

The exposure of subnuclear compartments of cells to ionizing radiation is currently not trivial. We describe here a collimator for micrometer-wide stripe irradiation designed to work with conventional high-voltage X-ray tubes and cells cultured on standard ...

In this contribution we describe the application of Ink-Jet printing and Stencil Lithography in bionanotechnology. Both techniques are alternative patterning methods that can be used for the fabrication of biocompatible micro- and nanostructures out of the ...

Kristopher Pataky

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Stencil Lithography and Inkjet Printing as New Tools for Life Sciences Research

Link between Alginate Reaction Front Propagation and General Reaction Diffusion Theory

NMR spectroscopy and perfusion of mammalian cells using surface microprobes

Fluidic microstructuring of alginate hydrogels for the single cell niche

An Oligomerized 53BP1 Tudor Domain Suffices for Recognition of DNA Double-Strand Breaks

High-Fidelity Printing Strategies for Printing 3D Vascular Hydrogel Structures

Etching of sub-micrometer structures through Stencil

Microcollimator for Micrometer-Wide Stripe Irradiation of Cells Using 20–30 keV X Rays

Nanostencil and InkJet Printing for Bionanotechnology Applications