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Aleksandra Radenovic

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Publications associées (153)

The emergence of nanopores in next-generation sequencing

Aleksandra Radenovic, Lorenz Jan Steinbock

Next-generation sequencing methods based on nanopore technology have recently gained considerable attention, mainly because they promise affordable and fast genome sequencing by providing long read lengths (5 kbp) and do not require additional DNA amplific ...

2015

Identification of single nucleotides in MoS2 nanopores

Andras Kis, Aleksandra Radenovic, Roman Bulushev, Sergey Khlybov, Jiandong Feng, Ke Liu, Dumitru Dumcenco

The size of the sensing region in solid-state nanopores is determined by the size of the pore and the thickness of the pore membrane, so ultrathin membranes such as graphene and single-layer molybdenum disulphide could potentially offer the necessary spati ...

Nature Publishing Group2015

Large-area MoS2 grown using H2S as the sulphur source

Andras Kis, Aleksandra Radenovic, Duncan Thomas Lindsay Alexander, Philippe Gillet, Oriol López Sánchez, Dmitry Ovchinnikov, Dumitru Dumcenco

We report on the growth of molybdenum disulphide (MoS2) using H2S as a gas-phase sulfur precursor that allows controlling the domain growth direction of domains in both vertical (perpendicular to the substrate plane) and horizontal (within the substrate pl ...

2015

Probing Rotational and Translational Diffusion of Nanodoublers in Living Cells on Microsecond Time Scales

Aleksandra Radenovic, Fabrizia Dutto, Lucas Delannoy, Sylvie Roke, Carlos Alberto Macias Romero, Marie Eve Pascale Didier, Vitalijs Zubkovs

Nonlinear microscopes have seen an increase in popularity in the life sciences due to their molecular and structural specificity, high resolution, large penetration depth, and volumetric imaging capability. Nonetheless, the inherently weak optical signals ...

American Chemical Society2014

Combination of Optical Tweezers with Nanocapillaries as System for Estimation of DNA/Ligand Interactions

Aleksandra Radenovic, Lorenz Jan Steinbock, Roman Bulushev

Cell Press2014

ComEA Is Essential for the Transfer of External DNA into the Periplasm in Naturally Transformable Vibrio cholerae Cells

Aleksandra Radenovic, Matteo Dal Peraro, Melanie Blokesch, Patrick Martin Seitz, Hasan Pezeshgi Modarres, Lorenz Jan Steinbock, Roman Bulushev

The DNA uptake of naturally competent bacteria has been attributed to the action of DNA uptake machineries resembling type IV pilus complexes. However, the protein(s) for pulling the DNA across the outer membrane of Gram-negative bacteria remain speculativ ...

Public Library Science2014

Probing the size of proteins with glass nanopores

Aleksandra Radenovic, Lely Feletti, Melanie Blokesch, Lorenz Jan Steinbock, Roman Bulushev, Swati Krishnan

Single molecule studies using nanopores have gained attention due to the ability to sense single molecules in aqueous solution without the need to label them. In this study, short DNA molecules and proteins were detected with glass nanopores, whose sensiti ...

Royal Soc Chemistry2014

Measurement of the Position-Dependent Electrophoretic Force on DNA in a Glass Nanocapillary

Aleksandra Radenovic, Lorenz Jan Steinbock, Roman Bulushev, Sergey Khlybov

The electrophoretic force on a single DNA molecule inside a glass nanocapillary depends on the opening size and varies with the distance along the symmetrical axis of the nanocapillary. Using optical tweezers and DNA-coated beads, we measured the stalling ...

American Chemical Society2014

Nanopore Integrated Nanogaps for DNA Detection

László Forró, Axel Fanget, Aleksandra Radenovic, Arnaud Magrez, Floriano Traversi, Sergey Khlybov

A high-throughput fabrication of sub-10 nm nanogap electrodes combined with solid-state nanopores is described. These devices should allow concomitant tunneling and ionic current detection of translocating DNA molecules. We report the optimal fabrication p ...

American Chemical Society2014

Atomically Thin Molybdenum Disulfide Nanopores with High Sensitivity for DNA Translocation

Andras Kis, Aleksandra Radenovic, Jiandong Feng, Ke Liu

Atomically thin nanopore membranes are considered to be a promising approach to achieve single base resolution with the ultimate aim of rapid and cheap DNA sequencing. Molybdenum disulfide (MoS2) is newly emerging as a material complementary to graphene du ...

Amer Chemical Soc2014

High throughput second harmonic imaging for label-free biological applications

Pierre Marquet, Pierre Magistretti, Pascal Jourdain, Aleksandra Radenovic, Sylvie Roke, Carlos Alberto Macias Romero, Marie Eve Pascale Didier, Vitalijs Zubkovs, Orly Bagunu Tarun

Second harmonic generation (SHG) is inherently sensitive to the absence of spatial centrosymmetry, which can render it intrinsically sensitive to interfacial processes, chemical changes and electrochemical responses. Here, we seek to improve the imaging th ...

Optical Society of America2014

Progress in quantitative single-molecule localization microscopy

Aleksandra Radenovic, Paolo Annibale, Marco Scarselli, Arun Shivanandan, Hendrik Georges Deschout

With the advent of single-molecule localization microscopy (SMLM) techniques, intracellular proteins can be imaged at unprecedented resolution with high specificity and contrast. These techniques can lead to a better understanding of cell functioning, as t ...

Springer2014

Electron Spin Resonance of Nitrogen-Vacancy Defects Embedded in Single Nanodiamonds in an ABEL Trap

Aleksandra Radenovic, Metin Kayci, Huan-Cheng Chang

Room temperature optically detected magnetic resonance of a single quantum object with nanoscale position control is an outstanding challenge in many areas, particularly in the life sciences. We introduce a novel approach to control the nitrogen-vacancy (N ...

Amer Chemical Soc2014

Shrinking Nanocapillaries to Low Noise Nanopores for Single Molecule Detection

Aleksandra Radenovic, Lorenz Jan Steinbock, Roman Bulushev, Swati Krishnan

Cell Press2014

Challenges in quantitative single molecule localization microscopy

Aleksandra Radenovic, Marco Scarselli, Arun Shivanandan, Hendrik Georges Deschout

Single molecule localization microscopy (SMLM), which can provide up to an order of magnitude improvement in spatial resolution over conventional fluorescence microscopy, has the potential to be a highly useful tool for quantitative biological experiments. ...

Elsevier2014

Detecting the translocation of DNA through a nanopore using graphene nanoribbons

Mohamed Malik Benameur, Andras Kis, Aleksandra Radenovic, Camille Alice Raillon, Floriano Traversi, Daria Krasnozhon, Sergey Khlybov, Ke Liu

Solid-state nanopores can act as single-molecule sensors and could potentially be used to rapidly sequence DNA molecules. However, nanopores are typically fabricated in insulating membranes that are as thick as 15 bases, which makes it difficult for the de ...

Nature Publishing Group2013

Controllable Shrinking and Shaping of Glass Nanocapillaries under Electron Irradiation

Aleksandra Radenovic, Lorenz Jan Steinbock

The ability to reshape nanopores and observe their shrinkage under an electron microscope is a powerful and novel technique. It increases the sensitivity of the resistive pulse sensing and enables to detect very short and small molecules. However, this has ...

American Chemical Society2013

MosaicIA: an ImageJ/Fiji plugin for spatial pattern and interaction analysis

Aleksandra Radenovic, Arun Shivanandan

Background: Analyzing spatial distributions of objects in images is a fundamental task in many biological studies. The relative arrangement of a set of objects with respect to another set of objects contains information about potential interactions between ...

BioMed Central2013

DNA Trans location through Low-Noise Glass Nanopores

Aleksandra Radenovic, Camille Alice Raillon, Lorenz Jan Steinbock, Roman Bulushev, Swati Krishnan

The effect of electron irradiation-induced shrinking on glass nanocapillaries with diameters ranging from 75 to 14 nm was analyzed by measuring the conductance characteristics with and without DNA translocation. We have investigated nanocapillary shrinking ...

American Chemical Society2013

Enlightening G-protein-coupled receptors on the plasma membrane using super-resolution photoactivated localization microscopy

Aleksandra Radenovic, Paolo Annibale, Marco Scarselli

The possibility to visualize and image the arrangement of proteins within the cell at the molecular level has always been an attraction for scientists in biological research. In particular, for signalling molecules such as GPCRs (G-protein-coupled receptor ...

Portland Press2013

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