Publications de Daniel Boss | EPFL Graph Search

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Digital holographic microscopy (DHM) is a noninvasive optical imaging technique that provides quantitative phase images of living cells. In a recent study, we showed that the quantitative monitoring of the phase signal by DHM was a simple label-free method ...

In this paper, we present an automated approach to quantify information about three-dimensional (3D) morphology, hemoglobin content and density of mature red blood cells (RBCs) using off-axis digital holographic microscopy (DHM) and statistical algorithms. ...

Quantitative phase (QP) images of red blood cells (RBCs), which are obtained by off-axis digital holographic microscopy, can provide quantitative information about three-dimensional (3D) morphology of human RBCs and the characteristic properties such as me ...

We present a method to automatically segment red blood cells (RBCs) visualized by digital holographic microscopy (DHM), which is based on the marker-controlled watershed algorithm. Quantitative phase images of RBCs can be obtained by using off-axis DHM alo ...

We present a novel technique for three-dimensional (3D) image processing of complex fields. It consists in inverting the coherent image formation by filtering the complex spectrum with a realistic 3D coherent transfer function (CTF) of a high-NA digital ho ...

Red blood cell (RBC) membrane fluctuations provide important insights into cell states. We present a spatial analysis of red blood cell membrane fluctuations by using digital holographic Microscopy (DHM). This interferometric and dye-free technique, posses ...

A dual-wavelength digital holographic microscope to measure absolute volume of living cells is proposed. The optical setup allows us to reconstruct two quantitative phase contrast images at two different wavelengths from a single hologram acquisition. When ...

The authors have developed a live-cell multimodality microscope combining epifluorescence with digital holographic microscopy; it has been implemented with a decoupling procedure allowing to separately measure from the quantitative phase important cell par ...

Red blood cells (RBCs) present unique reversible shape deformability, essential for both function and survival, resulting notably in cell membrane fluctuations (CMF). These CMF have been subject of many studies in order to obtain a better understanding of ...

We introduce a microscopic method that determines quantitative optical properties beyond the optical diffraction limit and allows direct imaging of unstained living biological specimens. In established holographic microscopy, complex fields are measured us ...

We propose to apply statistical clustering algorithms on a three-dimensional profile of red blood cells (RBCs) obtained through digital holographic microscopy (DHM). We show that two classes of RBCs stored for 14 and 38 days can be effectively classified. ...

Daniel Boss

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Determination of Transmembrane Water Fluxes in Neurons Elicited by Glutamate Ionotropic Receptors and by the Co-transporters KCC2 and NKCC1: A Digital Holographic Microscopy Study

Automated statistical quantification of three-dimensional morphology and mean corpuscular hemoglobin of multiple red blood cells

Automated quantitative analysis of 3D morphology and mean corpuscular hemoglobin in human red blood cells stored in different periods

Automated segmentation of multiple red blood cells with digital holographic microscopy

Realistic 3D coherent transfer function inverse filtering of complex fields

Spatial analysis of erythrocyte membrane fluctuations by digital holographic microscopy

Measurement of absolute cell volume, osmotic membrane water permeability, and refractive index of transmembrane water and solute flux by digital holographic microscopy

Developments in digital holographic microscopy to explore the role of astrocytes in brain water homeostasis

Cell Morphology and Intracellular Ionic Homeostasis explored with a Multimodal Approach combining Epifluorescence and Digital Holographic Microscopy

Spatially-Resolved Eigenmode Decomposition of Red Blood Cells Membrane Fluctuations Questions the Role of ATP in Flickering

Marker-free phase nanoscopy

Recognition and classification of red blood cells using digital holographic microscopy and data clustering with discriminant analysis

Red blood cell structure and dynamics explored with digital holographic microscopy