Publications de Harm-Anton Klok

Publications associées (193)

Dual and multi-stimuli responsive polymeric nanoparticles for programmed site-specific drug delivery

In the past decades, polymeric nanoparticles have emerged as a most promising and viable technology platform for targeted and controlled drug delivery. As vehicles, ideal nanoparticles are obliged to possess high drug loading levels, deliver drug to the sp ...

Elsevier Sci Ltd2013

Temperature-Controlled Masking/Unmasking of Cell-Adhesive Cues with Poly(ethylene glycol) Methacrylate Based Brushes

Harm-Anton Klok, Solenne Desseaux

Thin, thermoresponsive polymer coatings that allow to reversibly modulate cell adhesion and detachment are attractive substrates for cell sheet engineering. Usually, this is accomplished by applying thin poly(N-isopropylacrylamide) (PNIPAM) coatings, which ...

American Chemical Society2014

Fluorescence-Based and Fluorescent Label-Free Characterization of Polymer Nanoparticle Decorated T Cells

Demetri Psaltis, Harm-Anton Klok, Ahmed Bassam Sayed Ayoub Mohamed Emam, Tanja Thomsen

Cells are attractive carriers for the transport and delivery of nanoparticulate cargo. The use of cell-based carriers allows one to enhance control over the biodistribution of drug-loaded polymers and polymer nanoparticles. One key element in the developme ...

American Chemical Society2021

Caspofungin on ARGET-ATRP grafted PHEMA polymers: Enhancement and selectivity of prevention of attachment of Candida albicans

Harm-Anton Klok, Piotr Mocny

There is a need for coatings for biomedical devices and implants that can prevent the attachment of fungal pathogens while allowing human cells and tissue to appose without cytotoxicity. Here, the authors study whether a poly(2-hydroxyethylmethacrylate) (P ...

2017

Swelling and Degrafting of Poly(3-sulfopropyl methacrylate) Brushes

Harm-Anton Klok, Sabrina Sant-Greber, Kuljeet Kaur

Upon exposure to a good solvent, polymer brushes prepared via surface-initiated polymerization can undergo degrafting via cleavage of bonds that anchor the polymer tethers to the underlying substrate. As polymer brushes are often used in a solvent swollen ...

American Chemical Society (ACS)2024

Sequentially Modified, Polymer-Stabilized Gold Nanoparticle Libraries: Convergent Synthesis and Aggregation Behavior

Harm-Anton Klok, Maarten Danial

This manuscript describes a versatile, yet experimentally facile, method for producing libraries of polymer coated (core-shell type) gold nanoparticles. The synthetic principle relies on two, sequential postmodification reactions, which ensures homogeneity ...

2011

Arginine-Specific Modification of Proteins with Polyethylene Glycol

Harm-Anton Klok

In this study, the residue-selective modification of proteins with polymers at arginine residues is reported. The difficulty in modifying arginine residues lies in the fact that they are less reactive than lysine residues. Consequently, typical chemo-selec ...

American Chemical Society2011

Sustainable polyesters via direct functionalization of lignocellulosic sugars

Maxime Alexandre Clément Hedou, François Maréchal, Véronique Michaud, Yves Leterrier, Harm-Anton Klok, Jeremy Luterbacher, Adrien Julien Demongeot, Graham Reid Dick

The development of sustainable plastics from abundant renewable feedstocks has been limited by the complexity and efficiency of their production, as well as their lack of competitive material properties. Here we demonstrate the direct transformation of the ...

2022

Mechanical Acceleration of Ester Bond Hydrolysis in Polymers

Xiaobin Gao, Harm-Anton Klok, Anne-Clémence Corminboeuf, Jian Wang, Alice Boarino, Frédéric Célerse

Polymers containing ester bonds are ubiquitous. Often, these materials are mechanically challenged, which impacts their stability and degradation. While the accelerative effect of force on the degradation of polyester polymers is well recognized, little is ...

American Chemical Society2022

Light-Activated, Bioadhesive, Poly(2-hydroxyethyl methacrylate) Brush Coatings

Dominique Pioletti, Harm-Anton Klok, Jian Wang, Peyman Karami, Nariye Cavusoglu Ataman

Rapid adhesion between tissue and synthetic materials is relevant to accelerate wound healing and to facilitate the integration of implantable medical devices. Most frequently, tissue adhesives are applied as a gel or a liquid formulation. This manuscript ...

American Chemical Society2020

Reversibly Cross-Linking Polymer Brushes Using Interchain Disulfide Bonds

Harm-Anton Klok, Piotr Mocny

The introduction of interchain cross-links in surface-grafted polymer brushes increases the robustness and mechanical properties of these thin polymer films. In most cases, cross-linked polymer brushes contain permanent interchain cross-links. The use of r ...

American Chemical Society2020

A post-modification approach to peptide foldamers

Harm-Anton Klok, Laure Menin, Nadja Franz

Hydrophilic/hydrophobic patterning is a powerful strategy to control folding in non-natural polymers/oligomers. In this contribution, we present a novel strategy for the preparation of alternating hydrophilic/hydrophobic patterned non-natural peptide folda ...

Royal Society of Chemistry2009

Functional, Hyperbranched Polyesters Via Baylis-Hillman Polymerization

Harm-Anton Klok, Frederik Roman Wurm, Sanhao Ji

Hyperbranched polyesters are among the most common hyperbranched polymers. One of the interesting features of hyperbranched polyesters is that they contain unreacted hydroxyl and carboxylic acid groups at the linear and terminal structural units, which can ...

2012

Comparative Study on the In Vitro Cytotoxicity of Linear, Dendritic, and Hyperbranched Polylysine Analogues

Harm-Anton Klok, Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Zuzana Kadlecova

Lysine-based polycations are widely used as nonviral carriers for gene delivery. This manuscript reports the results of a comparative study on the in vitro cytotoxicity of a library of three structural polylysine variants, namely, linear polylysine (LPL), ...

American Chemical Society2012

Potential novel drug carriers for inner ear treatment: hyperbranched polylysine and lipid nanocapsules

Harm-Anton Klok, Markus Scholl, Zuzana Kadlecova

Aim: Treatment of sensorineural hearing loss could be advanced using novel drug carriers such as hyperbranched polylysine (HBPL) or lipid nanocapsules (LNCs). This study examined HBPL and LNCs for their cellular uptake and possible toxicity in vitro and in ...

Future Medicine2009

Site-Specific PEGylation of HR2 Peptides: Effects of PEG Conjugation Position and Chain Length on HIV-1 Membrane Fusion Inhibition and Proteolytic Degradation

Harm-Anton Klok, Maarten Danial

Peptides derived from the HR1 or HR2 regions of the HIV-1 envelope glycoprotein gp41 have been shown to be effective inhibitors to prevent virus-host cell membrane fusion. These peptide drugs, however, suffer from relatively short plasma half-lives and are ...

Amer Chemical Soc2012

Amphiphilic multi-arm star-block copolymers for encapsulation of fragrance molecules

Jan-Anders Edvin Maansson, Harm-Anton Klok, John Christopher Plummer, Céline Ternat

Novel amphiphilic multi-arm star-block copolymers with a hyperbranched core, a hydrophobic inner shell, and a hydrophilic outer shell have been prepared from a commercial hyperbranched polyester macroinitiator by ring-opening polymerization of -caprolacton ...

2007

Polymer–protein conjugates: an enzymatic activity perspective

Harm-Anton Klok

Proteins have been modified with polymers in diverse manners over the past 30 years. However, while proteins have been used to prepare many functional constructs, they are sensitive biomolecules and their bioactivity can be either positively or negatively ...

2010

Aqueous Fabrication of pH-Gated, Polymer-Brush-Modified Alumina Hybrid Membranes

Harm-Anton Klok, Caroline Sugnaux

In this Article, we studied the surface immobilization of five organic-acid-modified atom-transfer radical polymerization (ATRP) initiators based on salicylic acid, catechol, phthalic acid, and m- and p-benzoic add on alumina, and we also investigated the ...

Amer Chemical Soc2013

Degrafting of Poly(poly(ethylene glycol) methacrylate) Brushes from Planar and Spherical Silicon Substrates

Harm-Anton Klok, Nariye Cavusoglu Ataman

Densely grafted, hydrophilic polymer brushes produced via surface-initiated controlled radical polymerization have been shown to undergo degrafting upon exposure to aqueous media. This degrafting process has been proposed to involve swelling-induced, mecha ...

American Chemical Society2016