Preparation of modified RNA sequences for biological research

Our group is working on the development of reliable access to biol. relevant, long, and modified RNA sequences. Here, we briefly present the chem. synthesis of such sequences with 2'-O-triisopropylsilyloxymethyl (= 2'-O-tom) protected ribonucleoside phosphoramidites, their template-directed enzymic ligation, and some examples of modified nucleotides, designed to promote structural and biol. studies. [on SciFinder (R)]