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David Hacker

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Publications associées (82)

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Fluid dynamics of flow fields in a disposable 600-mL orbitally shaken bioreactor

Florian Maria Wurm, David Hacker, Dominique Terry Monteil Jr

Orbitally shaken bioreactors (OSRs) are commonly used for the cultivation of mammalian cells in suspension. Here we conducted a three-dimensional computational fluid dynamics (CFD) simulation to characterize the fluid field in the disposable 600-mL orbital ...

Elsevier2018

Innovative, Non-stirred Bioreactors in Scales from Milliliters up to 1000 Liters for Suspension Cultures of Cells using Disposable Bags and Containers – A Swiss Contribution

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Mathieu Benjamin Stettler

Innovative mixing principles in bioreactors, for example using the rocking of a platform to induce a backwards and forwards 'wave' , or using orbital shaking to generate a 'wave' that runs round in a cylindrical container, have proved to be successful for ...

Schweizerische Chemische Gesellschaft2010

The CELO adenovirus Gam1 protein enhances transient and stable recombinant protein expression in Chinese hamster ovary cells

Florian Maria Wurm, David Hacker

The Gam1 protein of the avian CELO adenovirus activates transcription through inhibition of histone deacetylase 1 (HDAC1). We investigated the effect of Gam1 on both transient and stable transgene expression in Chinese hamster ovary (CHO) cells, one of the ...

2005

Large-Scale Transfection of Mammalian Cells

Florian Maria Wurm, David Hacker, Lucia Baldi Unser

The large-scale transfection of mammalian cells allows moderate (milligram to gram) amounts of recombinant proteins (r-proteins) to be obtained for fundamental or clinical research. In this article, we describe a one-liter transfection using polyethyleneim ...

Humana Press2011

DNA delivery by microinjection for the generation of recombinant mammalian cell lines

Florian Maria Wurm, David Hacker, Madiha Derouazi, Sébastien Chenuet

Gene transfer methods for producing recombinant cell lines are often not very efficient. One reason is that the recombinant DNA is delivered into the cell cytoplasm and only a small fraction reaches the nucleus. This chapter describes a method for microinj ...

Humana Press2009

Transient gene expression: A novel mammalian cell-based technology for recombinant protein production. A historical and technical perspective

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Martin Jordan, Philippe Pierre Girard, Natalie Muller, Jason Wright, Frédéric Grosjean

Springer2002

BRB-seq: ultra-affordable high-throughput transcriptomics enabled by bulk RNA barcoding and sequencing

Bart Deplancke, David Hacker, Vincent Roland Julien Gardeux, Daniel Alpern, Bastien Mangeat, Antonio Carlos Alves Meireles Filho, Julie Marie Russeil

Despite its widespread use, RNA-seq is still too laborious and expensive to replace RT-qPCR as the default gene expression analysis method. We present a novel approach, BRB-seq, which uses early multiplexing to produce 3 cDNA libraries for dozens of sample ...

2019

Production and characterisation of rationally optimised, anti-melanoma T-cell receptors: a step closer towards immunotherapeutic treatment for patients

David Hacker

Swiss Medical Publishers Ltd / EMH Swiss Medical Publishers2008

The use of filler DNA for improved transfection and reduced DNA needs in transient gene expression with CHO and HEK cells

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Yashas Rajendra, Divor Kiseljak

2011

David Hacker

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Fluid dynamics of flow fields in a disposable 600-mL orbitally shaken bioreactor

Innovative, Non-stirred Bioreactors in Scales from Milliliters up to 1000 Liters for Suspension Cultures of Cells using Disposable Bags and Containers – A Swiss Contribution

The CELO adenovirus Gam1 protein enhances transient and stable recombinant protein expression in Chinese hamster ovary cells

Large-Scale Transfection of Mammalian Cells

DNA delivery by microinjection for the generation of recombinant mammalian cell lines

Transient gene expression: A novel mammalian cell-based technology for recombinant protein production. A historical and technical perspective

BRB-seq: ultra-affordable high-throughput transcriptomics enabled by bulk RNA barcoding and sequencing

Production and characterisation of rationally optimised, anti-melanoma T-cell receptors: a step closer towards immunotherapeutic treatment for patients

Pausing of mammalian cells by cold exposure, limits and opportunities

Rapid recombinant protein production from pools of transposon-generated CHO cells

Transient transfection of insect Sf-9 cells in TubeSpin® bioreactor 50 tubes

Chromatin 3D interactions mediate genetic effects on gene expression

Transposon mediated co-integration and co-expression of transgenes in CHO-DG44 cells

Hydrodynamic stress in orbitally shaken bioreactors

Mammalian cell culture productivity at large scale – from grams/liter to tens of grams/liter soon?

Transient gene expression with CHO cells in conditioned medium: a study using TubeSpin® bioreactors

The optimization of a rapid low-cost alternative of large-scale medium sterilization

Transient gene expression in suspension HEK-293 cells: application to large-scale protein production

Influence of glutamine on transient and stable recombinant protein production in CHO and HEK-293 cells

The use of filler DNA for improved transfection and reduced DNA needs in transient gene expression with CHO and HEK cells