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David Hacker

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Related publications (83)

Respiratory syncytial virus subunit vaccine based on a recombinant fusion protein expressed transiently in mammalian cells

Florian Maria Wurm, Lucia Baldi Unser, Sophie Nallet, David Hacker

Although respiratory syncytial virus (RSV) causes severe lower respiratory tract infection in infants and adults at risk, no RSV vaccine is currently available. In this report, efforts toward the generation of an RSV subunit vaccine using recombinant RSV f ...

2009

Production and characterisation of rationally optimised, anti-melanoma T-cell receptors: a step closer towards immunotherapeutic treatment for patients

David Hacker

Swiss Medical Publishers Ltd / EMH Swiss Medical Publishers2008

The kinetics of polyethylenimine-mediated transfection in suspension cultures of Chinese hamster ovary cells

Florian Maria Wurm, Martin Bertschinger, David Hacker, Jean Cevey

The kinetics of polyethylenimine (PEI)-mediated gene transfer at early times after transfection of Chinese hamster ovary (CHO) cell in suspension were investigated using a novel in vitro assay. Addition of an excess of competitor DNA to the culture medium ...

Humana Press Inc, Springer2008

Calcium phosphate transfection generates mammalian recombinant cell lines with higher specific productivity than polyfection

Florian Maria Wurm, Madiha Derouazi, David Hacker, Nicolas Jaccard, Sébastien Chenuet

Transfection with polyethylenimine (PEI) was evaluated as a method for the generation of recombinant Chinese hamster ovary (CHO DG44) cell lines by direct comparison with calcium phosphate-DNA coprecipitation (CaPO4) using both green fluorescent protein (G ...

2008

Recombinant therapeutic protein production in cultivated mammalian cells: current status and future prospects

Florian Maria Wurm, Lucia Baldi Unser, David Hacker

Recombinant therapeutic proteins produced in mammalian cells represent a major class of biopharmaceuticals. In recent years, their demand has increased dramatically and is now driving the development of a variety of improvements to maximize their expressio ...

Elsevier2008

Mild hypothermia improves transient gene expression yields several fold in Chinese hamster ovary cells

Florian Maria Wurm, Sophie Bouchet, David Hacker, Stéphanie Tissot, Jean Cevey, Sarah Wulhfard

Large-scale transient gene expression (TGE) in mammalian cells is a rapid method to generate recombinant proteins, but the volumetric productivity for secreted proteins is still more than an order of magnitude lower than the yields typically achieved with ...

2008

Recombinant protein production by large-scale transient gene expression in mammalian cells : state of the art and future perspectives

Florian Maria Wurm, Lucia Baldi Unser, David Hacker

The expansion of the biologics pipeline depends on the identification of candidate proteins for clinical trials. Speed is one of the critical issues, and the rapid production of high quality, research-grade material for preclinical studies by transient gen ...

Kluwer Academic Publishers, Springer2007

Novel orbital shake bioreactors for transient production of CHO derived IgGs

Florian Maria Wurm, Mathieu Benjamin Stettler, David Hacker, Xiaowei Zhang

Large-scale transient gene expression in mammalian cells is being developed for the rapid production of recombinant proteins for biochemical and preclinical studies. Here, the scalability of transient production of a recombinant human antibody in Chinese h ...

American Chemical Society2007

Scalable transient gene expression in Chinese hamster ovary cells in instrumented and non-instrumented cultivation systems

Florian Maria Wurm, Martin Jordan, Madiha Derouazi, Natalie Muller, David Hacker, Sarah Wulhfard

Cell expansion, gene transfer and protein production were all executed with a single serum-free, animal protein-free commercial medium designed for suspension-adapted Chinese hamster ovary cells (CHO DG44). This is a most important process to consider for ...

Kluwer Academic Publishers, Springer2007

Disassembly of polyethylenimine-DNA particles in vitro: implications for polyethylenimine-mediated DNA delivery

Florian Maria Wurm, Martin Jordan, Martin Bertschinger, David Hacker, Gaurav Backliwal

Here a simple in vitro assay was used to investigate the disassembly of nanoparticles of polyethylenimine (PEI) and DNA. Particles were formed with various PEIs, allowed to mature for 10 min, and then exposed to different competitors (RNA, DNA, BSA or hepa ...

2006

New disposable tubes for rapid and precise biomass assessment for suspension cultures of mammalian cells

Florian Maria Wurm, Martin Jordan, Mathieu Benjamin Stettler, David Hacker, Nicolas Jaccard

We present a new approach for biomass assessment in cell culture using a disposable microcentrifuge tube. The specially designed tube is fitted with an upper chamber for sample loading and a lower 5 microL capillary for cell collection during centrifugatio ...

2006

Multiple glycerol shocks increase the calcium phosphate transfection of non-synchronized CHO cells

Florian Maria Wurm, Frédéric Grosjean, Martin Bertschinger, David Hacker

The exposure of CHO DG44 cells to an osmotic shock, after DNA uptake, results in a cellular volume decrease of approx. 55%. Repetitive osmotic shocks targeted different sub-populations of cells as was demonstrated using two different fluorescent reporter g ...

2006

Genetic characterization of CHO production host DG44 and derivative recombinant cell lines

Florian Maria Wurm, Madiha Derouazi, Martin Bertschinger, David Hacker

The dihydrofolate reductase-deficient Chinese hamster ovary (CHO) cell line DG44 is the dominant mammalian host for recombinant protein manufacturing, in large part because of the availability of a well-characterized genetic selection and amplification sys ...

2006

Polyethylenimine-based quality control assay for plasmid DNA

Florian Maria Wurm, Martin Jordan, Martin Bertschinger, David Hacker, Gaurav Backliwal

2006

Orbital shaker technology for the cultivation of mammalian cells in suspension

Philippe Pierre Girard, Florian Maria Wurm, Martin Jordan, Natalie Muller, David Hacker

For large-scale applications in biotechnology, cultivation of mammalian cells in suspension is an essential prerequisite. Typically, suspension cultures are grown in glass spinner flasks filled to less than 50% of the nominal volume. We propose a superior ...

2005

Low-temperature pausing of cultivated mammalian cells

Lisa Hunt, Florian Maria Wurm, Martin Jordan, Frédéric Grosjean, David Hacker

There are currently two methods for maintaining cultured mammalian cells, continuous passage at 37 degrees C and freezing in small batches. We investigated a third approach, the "pausing" of cells for days or weeks at temperatures below 37 degrees C in a v ...

2005

Mammalian cell culture productivity at large scale – from grams/liter to tens of grams/liter soon?

Florian Maria Wurm, Martin Jordan, David Hacker

2005

The CELO adenovirus Gam1 protein enhances transient and stable recombinant protein expression in Chinese hamster ovary cells

Florian Maria Wurm, David Hacker

The Gam1 protein of the avian CELO adenovirus activates transcription through inhibition of histone deacetylase 1 (HDAC1). We investigated the effect of Gam1 on both transient and stable transgene expression in Chinese hamster ovary (CHO) cells, one of the ...

2005

Reduction of adenovirus E1A mRNA by RNAi results in enhanced recombinant protein expression in transiently transfected HEK293 cells

Florian Maria Wurm, Lucia Baldi Unser, Martin Bertschinger, David Hacker

Human embryonic kidney 293 (HEK293) cells, a widely used host for large-scale transient expression of recombinant proteins, are transformed with the adenovirus E1A and E1B genes. Because the E1A proteins function as transcriptional activators or repressors ...

2004

Transient gene expression in suspension HEK-293 cells: application to large-scale protein production

Philippe Pierre Girard, Florian Maria Wurm, Lucia Baldi Unser, David Hacker

Springer2003

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