Publications de Stefan Catsicas

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Many approaches have been developed to characterize the heterogeneity of membranes in living cells. In this study, the elastic properties of specific membrane domains in living cells are characterized by atomic force microscopy. Our data reveal the existen ...

We have determined the mechanical anisotropy of a single microtubule by simultaneously measuring the Young's and the shear moduli in vitro. This was achieved by elastically deforming the microtubule deposited on a substrate tailored by electron-beam lithography with a tip of an atomic force microscope. The shear modulus is 2 orders of magnitude lower than the Young's, giving rise to a length-dependent flexural rigidity of microtubules. The temperature dependence of the microtubule's bending stiffness in the

The atomic force microscope is a convenient tool to probe living samples at the nanometric scale. Among its numerous capabilities, the instrument can be operated as a namo-indenter to gather information about the mechanical properties of the sample. In thi ...

Central to the biological function of microtubules. is their ability to modify their length which occurs by addition and removal of subunits at the ends of the polymer, both in vivo and in vitro. This dynamic behavior is strongly influenced by temperature. ...

The cellular toxicity of carbon-based nanomaterials was studied as a function of their aspect ratio and surface chemistry. These structures were multiwalled carbon nanotubes, carbon nanofibers, and carbon nanoparticles. Their toxicity was tested in vitro o ...

A method of analyzing a sample with a probe based measurement instrument using stiffness tomography. Stiffness or elastic modulus data obtained through contact measurement with the sample as a function of depth is used in the detection of features, particu ...

Changes in mechanical properties of the cytoplasm have been implicated in cell motility, but there is little information about these properties in specific regions of the cell at specific stages of the cell migration process. Fish epidermal keratocytes wit ...

We report a comprehensive in vitro study of the photo-oxidative stress on different biomolecular and cellular targets generated in the presence of fullerol C-60(OH)(n), a novel, fullerene-based and water-soluble sensitizer of singlet oxygen ((1)Delta(g)). ...

Stefan Catsicas

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Elastic membrane heterogeneity of living cells revealed by stiff nanoscale membrane domains

Nanomechanics of Microtubules

Stiffness Tomography by Atomic Force Microscopy

Temperature-Dependent Elasticity of Microtubules

Cellular toxicity of carbon-based nanomaterials

Stiffness tomography by atomic force microscopy

Gradient of Rigidity in the Lamellipodia of Migrating Cells Revealed by Atomic Force Microscopy

Singlet oxygen ((1)Delta(g))-mediated oxidation of cellular and subcellular components: ESR and AFM assays

Superficial and deep changes of cellular mechanical properties following cytoskeleton disassembly

Interactions between NEEP21, GRIP1 and GluR2 regulate sorting and recycling of the glutamate receptor subunit GluR2

Characterization of MAP1B heavy chain interaction with actin

Large fluctuations in the disassembly rate of microtubules revealed by atomic force microscopy

Interactions between synaptic vesicle fusion proteins explored by atomic force microscopy

Oscillation modes of microtubules

Mechanical Properties of Microtubules Explored Using the Finite Elements Method

Fuzzy logic algorithm to extract specific interaction forces from atomic force microscopy data

Vitrification of cryoelectron microscopy specimens revealed by high-speed photographic imaging

Setup for observing living cells using a commercial atomic force microscope