Harald Hirling

since 2018:Adjoint du Doyen SV, Responsable Finance SVsince 2008:Adjoint du Doyen SV2002-2010:Adjoint scientifique, FSV-LNC, EPFL2006-2008:Adjoint de Section SSV2003-2004:coordinator (directeur) of the doctoral program in Neuroscience, EPFL Doctoral School1994-2001:University of LausanneStanford UniversityInstitut Curie, ParisEducation:ISREC, Epalinges (Switzerland).University of Konstanz (Germany)RECENT PUBLICATIONS - Steiner, P., Sarria, J.-C. F., Glauser, L., Magnin, S., Catsicas, S. and Hirling, H. (2002)Modulation of receptor cycling by Neuron-Enriched Endosomal Protein of 21 kDaJ. Cell Biol., 157, 1197-1209- Coppola, T., Frantz, C., Perret-Menoud, V., Gattesco, S., Hirling, H., and Regazzi, R. (2002)The pancreatic b-cell protein granuphilin binds Rab3 and Munc-18 and controls exocytosisMol. Biol. Cell, 13, 1906-1915- Sarria, J.-C. F., Catsicas, S., Hornung, J.-P. and Hirling, H. (2002)Developmental and spatial expression pattern of syntaxin 13 in the mouse central nervous systemCell Tissue Res., 309, 209-218- Steiner, P., Sarria, J.-C. F., Huni, B., Marsault, R., Catsicas, S. and Hirling, H. (2002)Overexpression of neuronal Sec1 enhances axonal branching in hippocampal neuronsNeuroscience, 113, 893-905- Morgenthaler, F.D., Knott, G., Sarria, J.-C. F., Wang, X., Staple, J.K., Catsicas, S. and Hirling, H. (2003)Morphological and molecular heterogeneity in release sites of single neuronsEur. J. Neurosci., 17, 1365-1374- Yersin, A., Hirling, H., Steiner, P., Magnin, S., Regazzi, R., Huni, B., Huguenot, P., De Los Rios, P., Dietler, G., Catsicas, S. and Kasas, S. (2003)Interactions between synaptic vesicle fusion proteins explored by atomic force microscopyProc. Natl. Acad. Sci. USA, 100, 8736-8741- Uldy, M., Steiner, P., Zürich-Graf, M.-G., Béguin, P., Hirling, H., Honneger, P. , Dolci, W. and Thorens, B. (2004)Regulated Exocytosis of a H /myo-Inositol symporter at synapses and growth conesEMBO J., 23, 531-540- Meijering, E., Jacob, M., Sarria, J.-C. F., Steiner, P., Hirling, H., and Unser, M. (2004)Design and Validation of a Tool for Neurite Tracing and Analysis in Fluorescence Microscopy ImagesCytometry, 58A,167-176- Steiner, P., Kulangara, K., Sarria, J.-C. F ., Glauser, L., Regazzi, R. and Hirling, H. (2004)Reticulon 1-C/Neuroendocrine-Specific Protein-C interacts with SNARE proteinsJ. Neurochem., 89, 569-580- Debaigt, C., Hirling, H., Vincent, J.-P., Steiner, P. and Mazella, J (2004)Crucial Role of Neuron-Enriched Endosomal Protein of 21 KD in sorting between degradation and recycling of internalized G-protein coupled receptorsJ. Biol. Chem, 279 (34), 35687-35691- Saxena, S., Howe, C.L., Cosgaya, J.M., Steiner, P., Hirling, H., Chan, J.R., Weis, J. and Krüttgen, A. (2005) Differential endocytic sorting of p75NTR and TrkA in response to NGF: a role for late endosomes in TrkA traffickingMol. Cell. Neurosci., 28, 571-587- Beretta, F., Sala, C., Saglietti, L., Hirling, H., Sheng, M. and Passafaro, M. (2005)NSF interaction is important for direct insertion of GluR2 at synaptic sitesMol. Cell. Neurosci., 28, 650-660- Alberi, S., Boda, B., Steiner, P., Nikonenko, I., Hirling, H. and Muller, D. (2005)Interference with the endosomal protein NEEP21 affects AMPA receptor mediated synaptic transmission and plasticity in the hippocampusMol. Cell. Neurosci., 29, 313-319- Kasas, S., Wang, X., Hirling, H., Marsault, R., Huni, B., Yersin, A., Regazzi, R., Grenningloh, G., Riederer, B., Forrò, L., Dietler, G. and Catsicas, S. (2005)Superficial and Deep Changes of Cellular Mechanical Properties Following Cytoskeleton DisassemblyCell Motil Cytoskeleton, 62, 124-132Steiner, P., Alberi, S, Kulangara, K., Yersin, A., Sarria, JCF., Regulier, E., Kasas, S., Dietler, G., Muller, D., Catsicas, S. and Hirling, H. (2005)Interactions between NEEP21, GRIP1 and GluR2 regulate sorting and recycling of the glutamate receptor subunit GluR2EMBO J. , 24, 2873-2884- Genoud, C., Quariauxx, C., Steiner, P., Hirling, H., Welker, E. and Knott, G.W. (2006)Plasticity of astrocyte coverage and glutamate transmporter expression in adult mouse cortexPLoS Biology, 4, e343- Brissoni, B., Agostini, L., Kropf, M., Martinon, F., Swoboda, V., Lippens, S., Everett, H., Aebi, N., Janssens, S., Meylan, E., Felbermann-Corti, M., Hirling, H., Gruenberg, J, Tschopp, J and Burns, K. (2006)Intracellular trafficking of Interleukin-1 receptor I requires TollipCurrent Biology, 16, 2265- Kulangara, K., Kropf, M., Glauser, L., Magnin, S., Alberi, S., Yersin, A. and Hirling, H. (2007)Phosphorylation of GRIP1 regulates surface expression of glutamate receptorsJ. Biol. Chem., 282 (4), 2395-2404- Yersin, A., Hirling, H., Kasas, S., Roduit, C., Kulangara, K., Dietler, G., Lafont, F., Catsicas, S. and Steiner, P. (2007)Elastic properties of the cell surface and trafficking of single AMPA receptors in living primary hippocampal neuronsBiophys. J., 92(12), 4482-4489- Brunner, J.-M., Plattet, P., Majcherczyk, P., Zurbriggen, A., Wittek, R. and Hirling, H. (2007)Canine distemper virus infection of primary hippocampal cells induces increase in extracellular glutamate and neurodegenerationJ. Neurochem., 103, 1184-1195- Yap, C.C., Wisco, D., Kujala, P., Lasiecka, Z.M., Cannon, J.T., Chang, M.C., Hirling, H., Klumperman, J. and Winckler, B. (2008)The somatodendritic endosomal regulator NEEP21 facilitates axonal targeting of L1/NgCAMJ. Cell Biol., 180, 827-842- Kropf, M.,, Rey, G., Glauser, L., Kulangara, K., Johnsson, K. and Hirling, H. (2008)Subunit-specific surface mobility of differentially labeled AMPA receptor subunitsEur. J. Cell. Biol., 87, 763-778-Rudinskiy, N., Grishchuk, Y., Vaslin, A., Puyal, J., Delacourte, A., Hirling, H., Clarke, P.G. and Luthi-Carter, R. (2009)Calpain hydrolysis of alpha- and beta2-adaptins decreases clathrin-dependent endocytosis and may promote neurodegeneration.J. Biol. Chem, 284, 12447-58- Utvik, J.K., Haglerød, C., Mylonakou, M.N., Holen, T., Kropf, M., Hirling, H., Skare, O., Laake, P., Ottersen, O.P., Haug, F.M. and Davanger, S. (2009)Neuronal enriched endosomal protein of 21 kDa colocalizes with glutamate receptor subunit GLUR2/3 at the postsynaptic membrane.Neuroscience, 158, 96-104- Mbefo, M.K., Paleologou, K.E., Boucharaba, A., Oueslati, A., Schell, H., Fournier, M., Olschewski, D., Yin, G., Zweckstetter, M., Masliah, E., Kahle, P.J., Hirling, H. and Lashuel, H.A. (2010)Phosphorylation of synucleins by members of the Polo-like kinase familyJ. Biol. Chem, 285, 2807-22TEACHING EXPERIENCE - "Introduction Biologie Cellulaire I" to 1. year chemistry students(EPFL, 2005-2006, 8h lecture per year, 8h exercises per year), in French- Histology of the Nervous Tissue and the Nervous system to 2. year medical students(University of Lausanne, 2000 – 2001, 8h per year), in French- Assistance in microscopy courses of General Histology to 2. year medical students(University of Lausanne, 1997 – 2001, 56h per year), in French- Problem-based Teaching in Biochemisty (Metabolism and Nutrition) to 2. year medical students(University of Lausanne, 2000 – 2002, 8h per year), in French- Cellular Neuroscience Module: "Intracellular Trafficking" to 3. year biology students(University of Lausanne, 2003 – 2004, 6h per year), in French- "Membrane Dynamics" and "Plasticity" lectures in orientation Neuroscience course to 3. year life sciences BA students(EPFL, 2005, 4h per year), in English- Master course "Biologie et biochimie cellulaire pour ingénieurs", to MA students in Microtechniques, Material Sciences, Informatics(EPFL, 2006 - 2011, 28h lecture per year, 28h exercises per year), in English- Graduate course "Developmental Neurobiology"; Doctoral Schools EPFL/UNIL in Neuroscience(EPFL/UniL, 2002 – 2006, 3h pro year), in English- Graduate course "Image acquisition and processing in biology", Doctoral Schools EPFL/UNIL in Neuroscience(EPFL/UniL, 2004-2005, 21h per year), in English