Publications de Giovanni Dietler

Publications associées (149)

Understanding amyloid aggregation by statistical analysis of atomic force microscopy images

Giovanni Dietler, Paolo De Los Rios, Raffaele Mezzenga

The aggregation of proteins is central to many aspects of daily life, including food processing, blood coagulation, eye cataract formation disease and prion-related neurodegenerative infections[1–5]. However, the physical mechanisms responsible for amyloid ...

Nature Publishing Group2010

A Rapid Unraveling of the Activity and Antibiotic Susceptibility of Mycobacteria

Giovanni Dietler, Sandor Kasas, Giovanni Longo, Leonardo Venturelli

The development of antibiotic-resistant bacteria is a worldwide health-related emergency that calls for new tools to study the bacterial metabolism and to obtain fast diagnoses. Indeed, the conventional analysis time scale is too long and affects our abili ...

AMER SOC MICROBIOLOGY2019

Kinetics of Antibody Binding to Membranes of Living Bacteria Measured by a Photonic Crystal-Based Biosensor

Giovanni Dietler, Serguei Sekatski, Ekaterina Rostova

Optical biosensors based on photonic crystal surface waves (PC SWs) offer a possibility to study binding interactions with living cells, overcoming the limitation of rather small evanescent field penetration depth into a sample medium that is characteristi ...

Mdpi Ag2016

Identification of Oxidative Stress in Red Blood Cells with Nanoscale Chemical Resolution by Infrared Nanospectroscopy

Giovanni Dietler, Giovanni Longo, Francesco Simone Ruggeri

During their lifespan, Red blood cells (RBC), due to their inability to self-replicate, undergo an ageing degradation phenomenon. This pathway, both in vitro and in vivo, consists of a series of chemical and morphological modifications, which include devia ...

2018

Amyloid Single Cell Cytotoxicity Assays by Nanomotion Detection

Giovanni Dietler, Hilal Lashuel, Sandor Kasas, Giovanni Longo, Francesco Simone Ruggeri, Anne-Laure Mahul Mellier

Cells are extremely complex systems able to modify actively their metabolism and behaviour in response to environmental conditions and stimuli, such as pathogenic agents or drugs. The comprehension of these responses is central to understand the molecular ...

Springer Nature2017

Combination of fluorescence microscopy and nanomotion detection to characterize bacteria

Giovanni Dietler, Sandor Kasas, Giovanni Longo, Justin Terence Notz Pekkanen

Antibiotic-resistant pathogens are a major health concern in everyday clinical practice. Because their detection by conventional microbial techniques requires minimally 24h, some of us have recently introduced a nanomechanical sensor, which can reveal moti ...

Wiley-Blackwell2013

Environmental Control of Amyloid Polymorphism by Modulation of Hydrodynamic Stress

Giovanni Dietler, François Gallaire, Sandor Kasas, Serguei Sekatski, Jiangtao Zhou, Leonardo Venturelli, Ludovic Pierre Gustave Keiser

The phenomenon of amyloid polymorphism is a key feature of protein aggregation. Unravelling this phenomenon is of great significance for understanding the underlying molecular mechanisms associated with neurodegenerative diseases and for the development of ...

AMER CHEMICAL SOC2021

Force-induced globule-coil transition in laminin binding protein and its role for viral-cell membrane fusion

Giovanni Dietler, Serguei Sekatski, Fabrizio Benedetti, Andrey Mikhaylov, Tatyana Karakouz

The specific interactions of the pairs laminin binding protein (LBP)-purified tick-borne encephalitis viral surface protein E and certain recombinant fragments of this protein, as well as West Nile viral surface protein E and certain recombinant fragments ...

Wiley-Blackwell2014

Nanomechanical sensor applied to blood culture pellets: a fast approach to determine the antibiotic susceptibility against agents of bloodstream infections

Giovanni Dietler, Sandor Kasas, Giovanni Longo, Petar Stupar

Objectives: The management of bloodstream infection, a life-threatening disease, largely relies on early detection of infecting microorganisms and accurate determination of their antibiotic susceptibility to reduce both mortality and morbidity. Recently we ...

2017

Force spectroscopy of the fibrin(ogen) - Fibrinogen interaction

Giovanni Dietler

Fibrin aggregation is of vital importance in many physiological and pathological processes, such as blood coagulation, wound healing, and thrombosis. In the present study, we investigated the forces involved in the initial steps of the fibrinogen fibrin ag ...

2008

Atomic Force Microscopy Stiffness Tomography on Living Arabidopsis thaliana Cells Reveals the Mechanical Properties of Surface and Deep Cell-Wall Layers during Growth

Giovanni Dietler, Sandor Kasas, Charles Roduit

Cell-wall mechanical properties play a key role in the growth and the protection of plants. However, little is known about genuine wall mechanical properties and their growth-related dynamics at subcellular resolution and in living cells. Here, we used ato ...

Elsevier2012

Nuclear Factor I genomic binding associates with chromatin boundaries

Giovanni Dietler, Philipp Bucher, Giovanna Ambrosini, Nicolas Mermod, Petar Pjanic

Background: The Nuclear Factor I (NFI) family of DNA binding proteins (also called CCAAT box transcription factors or CTF) is involved in both DNA replication and gene expression regulation. Using chromatin immuno-precipitation and high throughput sequenci ...

BioMed Central2013

Characterization of glycoprotein E C-End of West Nile virus and evaluation of its interaction force with alpha V beta 3 integrin as putative cellular receptor

Giovanni Dietler, Serguei Sekatski

Recombinant polypeptide containing the 260-466 amino acid sequence of West Nile virus (WNV) strain LEIV-Vlg99-27889-human glycoprotein E (gpE, E260-466) was constructed. Immunochemical similarity between the E260-466 and gpE of WNV was proven by enzyme imm ...

2010

The role of xanthophylls in the supramolecular organization of the photosynthetic complex LHCII in lipid membranes studied by high-resolution imaging and nanospectroscopy

Giovanni Dietler, Serguei Sekatski, Jiangtao Zhou

The xanthophyll cycle is a regulatory mechanism operating in the photosynthetic apparatus of plants. It consists of the conversion of the xanthophyll pigment violaxanthin to zeaxanthin, and vice versa, in response to light intensity. According to the curre ...

ELSEVIER2020

Registration of long-range surface plasmon resonance by angle-scanning feedback and its implementation for optical hydrogen sensing

Giovanni Dietler, Serguei Sekatski

An optical technique devised for the detection of the ultrasharp angular resonance of long-range surface plasmons (LRSPs) is described. The LRSPs propagate along an 8 nm-thick palladium (Pd) film deposited on a one- dimensional photonic crystal structure a ...

Institute of Physics (IoP) and Deutsche Physikalische Gesellschaft2009

AFM-Based Single Molecule Techniques: Unraveling the Amyloid Pathogenic Species

Giovanni Dietler, Francesco Simone Ruggeri, Andrea Cerreta

Background: A wide class of human diseases and neurodegenerative disorders, such as Alzheimer's disease, is due to the failure of a specific peptide or protein to keep its native functional conformational state and to undergo a conformational change into a ...

Bentham Science Publishers2016

The persistence length of adsorbed dendronized polymers

Giovanni Dietler, Plinio Maroni, Andrey Mikhaylov

The persistence length of cationic dendronized polymers adsorbed onto oppositely charged substrates was studied by atomic force microscopy (AFM) and quantitative image analysis. One can find that a decrease in the ionic strength leads to an increase of the ...

Royal Society of Chemistry2016

Nanoscale spatially resolved infrared spectra from single microdroplets

Giovanni Dietler, Andrzej Kulik, Francesco Simone Ruggeri

Droplet microfluidics has emerged as a powerful platform allowing a large number of individual reactions to be carried out in spatially distinct microcompartments. Due to their small size, however, the spectroscopic characterisation of species encapsulated ...

Royal Society of Chemistry2014

The Nt17 Domain and its Helical Conformation Regulate the Aggregation, Cellular Properties and Neurotoxicity of Mutant Huntingtin Exon 1

Giovanni Dietler, Hilal Lashuel, Anass Chiki, Francesco Simone Ruggeri, Anne-Laure Mahul Mellier, Urszula Beata Cendrowska, Sophie Vieweg, Nathan Alain Denis Riguet, Sean Michael Deguire

Converging evidence points to the N-terminal domain comprising the first 17 amino acids of the Huntingtin protein (Nt17) as a key regulator of its aggregation, cellular properties and toxicity. In this study, we further investigated the interplay between N ...

Elsevier2021

Simulations of electrophoretic collisions of DNA knots with gel obstacles

Giovanni Dietler, Paolo De Los Rios, Cédric Weber

Gel electrophoresis can be used to separate nicked circular DNA molecules of equal length but forming different knot types. At low electric fields, complex knots drift faster than simpler knots. However, at high electric field the opposite is the case and ...

Institute of Physics2006