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Kai Johnsson

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Publications associées (125)

NCCR Chemical Biology: Interdisciplinary Research Excellence, Outreach, Education, and New Tools for Switzerland

Kai Johnsson, Howard Riezman

Funded by the Swiss National Science Foundation to promote cutting edge research as well as the advancement of young researchers and women, technology transfer, outreach and education, the NCCR (Swiss National Centre of Competence in Research) Chemical Bio ...

2011

Real-Time Measurements of Protein Dynamics Using Fluorescence Activation-Coupled Protein Labeling Method

Kai Johnsson

We present a fluorescence activation-coupled protein labeling (FAPL) method, which employs small-molecular probes that exhibit almost no basal fluorescence but acquire strong fluorescence upon covalent binding to tag-proteins. This method enables real-time ...

2011

Visualizing Biochemical Activities in Living Cells through Chemistry

Kai Johnsson, Luc Reymond, Matthias Albert Brun, Karolina Bojkowska, Birgit Mollwitz, Grazvydas Lukinavicius, Anastasiya Masharina, Alberto Schena, Keitaro Umezawa, Rudolf Griss

The development of molecular probes to visualize cellular processes is an important challenge in chemical biology. One possibility to create such cellular indicators is based on the selective labeling of proteins with synthetic probes in living cells. Over ...

2011

Compositions and use of sulfasalazine

Kai Johnsson, Christopher Chidley

The invention relates to compositions comprising sulfasalazine or sulfapyridine and products of a biosynthetic pathway that requires tetrahydrobiopterin. Such compositions are useful for the treatment of inflammatory bowel diseases or rheumatoid arthritis, ...

2011

Chemical Biology Approaches to Membrane Homeostasis and Function

Gerardo Turcatti, Kai Johnsson, Luc Reymond, Christian Heinis, Stefan Matile, Howard Riezman

The study of membranes is at a turning point. New theories about membrane structure and function have recently been proposed, however, new technologies, combining chemical, physical, and biochemical approaches are necessary to test these hypotheses. In par ...

2011

Semisynthesis of fluorescent metabolite sensors on cell surfaces

Horst Vogel, Kai Johnsson, Joachim Piguet, Luc Reymond, Matthias Albert Brun, Kui Thong Tan, Ruud Peters, Rudolf Griss

Progress in understanding signal transduction and metabolic pathways is hampered by a shortage of suitable sensors for tracking metabolites, second messengers, and neurotransmitters in living cells. Here we introduce a class of rationally designed semisynt ...

2011

Measuring In Vivo Protein Half-Life

Kai Johnsson, Christian Heinis, Didier Trono, Isabelle Barde, Sonia Verp, Karolina Bojkowska, Francesca Santoni de Sio, Sandra Eloise Kjeldsen

Protein turnover critically influences many biological functions, yet methods have been lacking to assess this parameter in vivo. Here, we demonstrate how chemical labeling of SNAP-tag fusion proteins can be exploited to measure the half-life of resident i ...

Elsevier2011

A yeast-based screen reveals that sulfasalazine inhibits tetrahydrobiopterin biosynthesis

Kai Johnsson, Christopher Chidley, Evelyne Muller, Miriam Grønlund Pedersen, Hirohito Haruki

2011

Searching for the Protein Targets of Bioactive Molecules

Kai Johnsson, Christopher Chidley, Cindy Fellay, Miriam Grønlund Pedersen, Simone Moser, Hirohito Haruki

The identification of all protein targets of a given drug or bioactive molecule within the human body is a prerequisite for an understanding of its beneficial and deleterious activities. Current approaches to reveal protein targets often fail to reveal phy ...

Schweizerische Chemische Gesellschaft2011

Targeted Photoswitchable Probe for Nanoscopy of Biological Structures

Iwan Märki, Theo Lasser, Kai Johnsson, Claudio Dellagiacoma, Stefan Geissbühler, Sambashiva Banala, Grazvydas Lukinavicius, Noelia Laura Bocchio

We introduce a photoswitchable O6-benzylguanine derivative and demonstrate its use for super-resolution microscopy of SNAP-tagged proteins based on single fluorophore localization. Stochastic Optical Reconstruction Microscopy (STORM) reveals SNAP-tagged mi ...

2010

Semisynthetic fluorescent sensor proteins based on self-labeling protein tags

Kai Johnsson, Matthias Albert Brun, Marlon Hinner, Kui Thong Tan

Genetically encoded fluorescent sensor proteins offer the possibility to probe the concentration of key metabolites in living cells. The approaches currently used to generate Such fluorescent sensor proteins lack. generality, as they require a protein that ...

2009

Visualizing biochemical activities in living cells

Kai Johnsson

Autofluorescent proteins have become indispensable in our quest to visualize molecular events in living cells. Further progress in the visualization and quantification of all biochemical activities of the cell will require the introduction of additional an ...

2009

Indo-1 derivatives for local calcium sensing

Kai Johnsson, Cindy Fellay, Annina Aebischer

The role of calcium in signal transduction relies on the precise spatial and temporal control of its concentration. The existing means to detect fluctuations in Ca2+ concentrations with adequate temporal and spatial resolution are limited. We introduce her ...

American Chemical Society2009

A designed protein for the specific and covalent hetero-conjugation of biomolecules

Kai Johnsson, Christopher Chidley, Katarzyna Mosiewicz

2008

Method of crosslinking two objects of interest

Kai Johnsson, Christopher Chidley, Katarzyna Mosiewicz

The invention provides a method of crosslinking two objects of interest, comprising the steps of : i) providing a fusion protein comprising at least a first protein and a second protein, wherein both the first and the second protein are, based on their str ...

2008

Labelling of fusion proteins with synthetic probes

Kai Johnsson, Alexandre Juillerat

The invention relates to new proteins called alkylcytosine transferases (ACTs) derived from O-alkylguanine-DNA alkyltransferase, and to substrates for ACTs specifically transferring a label to these ACTs and to fusion proteins comprising these. The subs ...

2008

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Kai Johnsson

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NCCR Chemical Biology: Interdisciplinary Research Excellence, Outreach, Education, and New Tools for Switzerland

Real-Time Measurements of Protein Dynamics Using Fluorescence Activation-Coupled Protein Labeling Method

Visualizing Biochemical Activities in Living Cells through Chemistry

Compositions and use of sulfasalazine

Chemical Biology Approaches to Membrane Homeostasis and Function

Semisynthesis of fluorescent metabolite sensors on cell surfaces

Measuring In Vivo Protein Half-Life

A yeast-based screen reveals that sulfasalazine inhibits tetrahydrobiopterin biosynthesis

Searching for the Protein Targets of Bioactive Molecules

Targeted Photoswitchable Probe for Nanoscopy of Biological Structures

Semisynthetic fluorescent sensor proteins based on self-labeling protein tags

Visualizing biochemical activities in living cells

Indo-1 derivatives for local calcium sensing

A designed protein for the specific and covalent hetero-conjugation of biomolecules

Method of crosslinking two objects of interest

Labelling of fusion proteins with synthetic probes

Regulation of glutamate metabolism by protein kinases in mycobacteria

A split-protein sensor for studying protein-protein interactions in mycobacteria

Caged substrates for protein labeling and immobilization

Subunit-specific surface mobility of differentially labeled AMPA receptor subunits