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Kai Johnsson

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Publications associées (125)

NanoSIMS analysis of an isotopically labelled organometallic ruthenium(II) drug to probe its distribution and state in vitro

Kai Johnsson, Paul Joseph Dyson, Graham Knott, Mary-Claude Croisier-Coeytaux, Anders Meibom, Stéphane Laurent Escrig

Royal Society of Chemistry2015

Modulating protein-protein interaction with a molecular tether

Kai Johnsson, Oliver Hantschel, Helen Rebecca Ostlund Farrants

Wiley-Blackwell2015

SiR–Hoechst is a far-red DNA stain for live-cell nanoscopy

Kai Johnsson, Luc Reymond, Grazvydas Lukinavicius, Alberto Schena

Cell-permeable DNA stains are popular markers in live-cell imaging. Currently used DNA stains for live-cell imaging are either toxic, require illumination with blue light or are not compatible with super-resolution microscopy, thereby limiting their utilit ...

Nature Research2015

A fluorogenic probe for SNAP-tagged plasma membrane proteins based on the solvatochromic molecule Nile Red

Rudolf Hovius, Kai Johnsson, Luc Reymond, Howard Riezman, Efthymia Prifti

A fluorogenic probe for plasma membrane proteins based on the dye Nile Red and SNAP-tag is introduced. It takes advantage of Nile Red, a solvatochromic molecule highly fluorescent in an apolar environment, such as cellular membranes, but almost dark in a p ...

Amer Chemical Soc2014

Protein tag-mediated conjugation of oligonucleotides to recombinant affinity binders for proximity ligation

Kai Johnsson

While antibodies currently play a dominant role as affinity reagents in biological research and for diagnostics, a broad range of recombinant proteins are emerging as promising alternative affinity reagents in detection assays and quantification. DNA-media ...

Elsevier2013

Labelling of Fusion Proteins with Synthetic Probes

Kai Johnsson, Alexandre Juillerat

The invention relates to new proteins called alkylcytosine transferases (ACTs) derived from O6-alkylguanine-DNA alkyltransferase, and to substrates for ACTs specifically transferring a label to these ACTs and to fusion proteins comprising these. The substr ...

2013

Sensing Acetylcholine and Anticholinesterase Compounds

Kai Johnsson, Alberto Schena

Acetylcholine is a key neurotransmitter, and anticholinesterase agents are essential compounds used as medical drugs, pesticides, and chemical warfare agents. A semisynthetic fluorescence-based probe for the direct, real-time detection of acetylcholine and ...

Wiley-Blackwell2013

Tetrahydrobiopterin Biosynthesis as an Off-Target of Sulfa Drugs

Kai Johnsson, Florence Pojer, Miriam Grønlund Pedersen, Hirohito Haruki, Katarzyna Irena Gorska

The introduction of sulfa drugs for the chemotherapy of bacterial infections in 1935 revolutionized medicine. Although their mechanism of action is understood, the molecular bases for most of their side effects remain obscure. Here, we report that sulfamet ...

American Association for the Advancement of Science2013

Identification of a small molecule with activity against drug-resistant and persistent tuberculosis (vol 110, pg E2510, 2013)

Kai Johnsson, Claudia Trefzer, Feng Wang, Yong Zhang, Jianing Wang

National Academy of Sciences2013

Selective Chemical Crosslinking Reveals a Cep57-Cep63-Cep152 Centrosomal Complex

Kai Johnsson, Luc Reymond, Pierre Gönczy, Grazvydas Lukinavicius, Keitaro Umezawa

The centrosome functions as the main microtubule-organizing center of animal cells and is crucial for several fundamental cellular processes [1]. Abnormalities in centrosome number and composition correlate with tumor progression [2, 3] and other diseases ...

Elsevier2013

Investigating Endocytic Pathways to the Endoplasmic Reticulum and to the Cytosol Using SNAP-Trap

Kai Johnsson

Cholera toxin enters cells via an unusual pathway that involves trafficking through endosomes to the endoplasmic reticulum (ER). Whether the toxin induces its own pathway or travels along a physiological retrograde route is not known. To study its traffick ...

Wiley-Blackwell2013

Cell permeable, fluorescent dye

Kai Johnsson, Grazvydas Lukinavicius, Keitaro Umezawa

The invention pertains to a near-infrared fluorescent dye that is cell permeable and can be attached to selected proteins in living cells. The dye has the general formula (I) or its or its corresponding spirolactone (II) wherein Y is chosen from the group ...

2013

Substrates for Improved Live-Cell Fluorescence Labeling of SNAP-tag

Kai Johnsson, Luc Reymond, Grazvydas Lukinavicius

The SNAP-tag labeling technology provides a simple, robust, and versatile approach to the imaging of fusion proteins for a wide range of experimental applications. Owing to the specific and covalent nature of the labeling reaction, SNAP-tag is well suited ...

Bentham Science Publ Ltd2013

Yeast Three-Hybrid Screening for Identifying Anti-Tuberculosis Drug Targets

Kai Johnsson, Simone Moser

Mycobacterium goes yeast: Target deconvolution of anti-tuberculosis drugs can be a very challenging task. Here we report a yeast 3-hybrid system that allows promising small molecules to be screened for protein targets of a pathogen in nontoxic yeast cells. ...

Wiley-V C H Verlag Gmbh2013

A Fluorescent Sensor for GABA and Synthetic GABA(B) Receptor Ligands

Kai Johnsson, Luc Reymond, Anastasiya Masharina, Keitaro Umezawa

While gamma-aminobutyric acid (GABA) is the main inhibitory neurotransmitter, suitable tools to measure its concentration in living cells with high spatiotemporal resolution are missing. Herein, we describe the first ratiometric fluorescent sensor for GABA ...

American Chemical Society2012

A Semisynthetic Fluorescent Sensor Protein for Glutamate

Kai Johnsson, Luc Reymond, Matthias Albert Brun, Kui Thong Tan, Rudolf Griss

We report the semisynthesis of a fluorescent glutamate sensor protein on cell surfaces. Sensor excitation at 547 nm yields a glutamate-dependent emission spectrum between 550 and 700 nm that can be exploited for ratiometric sensing. On cells, the sensor di ...

2012

Triplet imaging of oxygen consumption during the contraction of a single smooth muscle cell (A7r5)

Iwan Märki, Marcel Leutenegger, Theo Lasser, Kai Johnsson, Matthias Geissbühler, Dimitri Nestor Alice Van De Ville, Boris Hinz, Thiemo Spielmann, Aurélie Formey de Saint Louvent

Triplet imaging is a novel optical technique that allows investigating oxy- gen metabolism at the single cell and the sub-cellular level. The method combines high temporal and spatial resolution, which are required for the monitoring of fast kinetics of ox ...

Springer Science+Business Media2012

Exploiting Ligand-Protein Conjugates to Monitor Ligand-Receptor Interactions

Kai Johnsson, Monica Rengifo Gonzalez, Hirohito Haruki

We introduce three assays for analyzing ligand-receptor interactions based on the specific conjugation of ligands to SNAP-tag fusion proteins. Conjugation of ligands to different SNAP-tag fusions permits the validation of suspected interactions in cell ext ...

Public Library of Science2012

Directed evolution of the suicide protein O⁶-alkylguanine-DNA alkyltransferase for increased reactivity results in an alkylated protein with exceptional stability

Kai Johnsson, Ursula Röthlisberger, Simone Schmitt, Florence Pojer, Birgit Mollwitz, Elizabeth Claire Brunk

Here we present a biophysical, structural, and computational analysis of the directed evolution of the human DNA repair protein O-6-alkylguanine-DNA alkyltransferase (hAGT) into SNAP-tag, a self-labeling protein tag. Evolution of hAGT led not only to incre ...

2012

Liver-specific ablation of Krüppel-associated box-associated protein 1 in mice leads to male-predominant hepatosteatosis and development of liver adenoma

Kai Johnsson, Didier Trono, Karolina Bojkowska, Francesca Santoni de Sio, Sandra Eloise Kjeldsen, Adamantia Kapopoulou, Nadine Fournier, Cristina Cartoni, Marco Cassano, Fabio Aloisio

Wiley-Blackwell2012

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