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Martinus Gijs

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Publications associées (370)

Method for quantitative measurement of a biomarker by in situ immunofluorescence and uses thereof

Martinus Gijs, Déborah Heintze, Ata Tuna Ciftlik, Diego Gabriel Dupouy

The invention relates to a method for quantitative measurement of a biomarker by in situ immunofluorescence and uses thereof. In particular, the invention relates to a method which is a useful tool for use in the field of diagnosis, prevention and/or treat ...

2017

Microfluidics for rapid cytokeratin immunohistochemical staining in frozen sections

Martinus Gijs, Diego Gabriel Dupouy, Saska Brajkovic

Frozen sections (FS) of tumor samples represent a cornerstone of pathological intraoperative consultation and have an important role in the microscopic analysis of specimens during surgery. So far, immunohistochemical (IHC) stainings on FS have been demons ...

Nature Publishing Group2017

Microfluidics-assisted fluorescence in situ hybridization for advantageous human epidermal growth factor receptor 2 assessment in breast cancer

Martinus Gijs, Raphaël Etienne Jean Trouillon, Huu Tuan Nguyen, Maryse Fiche, Seiya Matsuoka

Fluorescence in situ hybridization (FISH) is one of the recommended techniques for human epidermal growth factor receptor 2 (HER2) status assessment on cancer tissues. Here we develop microfluidics-assisted FISH (MA-FISH), in which hybridization of the FIS ...

Nature Publishing Group2017

Microsphere-based super-resolution scanning optical microscope

Martinus Gijs, Hui Yang, Gergely Huszka

High-refractive index dielectric microspheres positioned within the field of view of a microscope objective in a dielectric medium can focus the light into a so-called photonic nanojet. A sample placed in such nanojet can be imaged by the objective with su ...

Optical Soc Amer2017

An Integrated Microfluidic Device For C-Elegans Early Embryogenesis Studies And Drug Assays

Thomas Lehnert, Martinus Gijs, Pierre Gönczy, Matteo Cornaglia, Li Dong, Radek Jankele, Jingwei Zhang

The Caenorhabditis elegans embryo is a widely used model for the functional analysis of dynamic cellular processes, such as chromosome segregation, cytokinesis or lineage analysis. However, the conventional embryo preparation method that relies on manually ...

Ieee2017

Microfluidic systems for high-throughput and high-content screening using the nematode Caenorhabditis elegans

Thomas Lehnert, Martinus Gijs, Matteo Cornaglia

In a typical high-throughput drug screening (HTS) process, up to millions of chemical compounds are applied to cells cultured in well plates, aiming to find molecules that exhibit a robust dose-response, as evidenced for example by a fluorescence signal. I ...

Royal Soc Chemistry2017

A multiscale study of the role of dynamin in the regulation of glucose uptake

Martinus Gijs, Kristina Schoonjans, Johan Auwerx, Laurent Mouchiroud, Keir Joe Menzies, Raphaël Etienne Jean Trouillon, Maria Cristina Letizia

Glucose uptake in muscle cells in response to insulin is a fundamental mechanism for metabolism. The inability of cells to mobilize the specific glucose transporter GLUT4 is believed to be at least partially accountable for diseases, like diabetes, where c ...

Royal Soc Chemistry2017

C. Elegans Immobilization Using Deformable Microfluidics For In Vivo Studies Of Early Embryogenesis And Intestinal Microbiota

Thomas Lehnert, Martinus Gijs, Matteo Cornaglia, Li Dong, Xiaopeng Huang, Jingwei Zhang

The genome of the nematode Caenorhabditis elegans shows far-reaching similarities to that of humans. C. elegans is therefore considered as a powerful model organism in biological research related to humans, for instance for the study of human diseases. Suc ...

Ieee2017

A microfluidic platform for automated phenotyping during full lifespan of Caenorhabditis elegans at high-throughput

Martinus Gijs, Johan Auwerx, Matteo Cornaglia, Laurent Mouchiroud, Hüseyin Baris Atakan

We present a multiplexed and automated microfluidic platform for phenotyping of C. elegans during their full lifespan and at high-throughput. We used the fluorescent hsp-6::gfp strain of worms and performed experiments to observe the effect of four differe ...

2017

System for optical detection and imaging of sub-diffraction-limited nano-objects

Martinus Gijs, Hui Yang, Matteo Cornaglia

Nano-objects (8) with feature size smaller than the classical Abbe's diffraction limit can be optically detected via a microlens (4) array configured in a miniaturized microfluidic device. A hydrophilic microwell (3) array in a hydrophobic layer (2) is fir ...

2016

Continuous quantification of HER2 expression by microfluidic precision immunofluorescence estimates HER2 gene amplification in breast cancer

Martinus Gijs, Déborah Heintze, Ata Tuna Ciftlik, Diego Gabriel Dupouy, Maryse Fiche

Chromogenic immunohistochemistry (IHC) is omnipresent in cancer diagnosis, but has also been criticized for its technical limit in quantifying the level of protein expression on tissue sections, thus potentially masking clinically relevant data. Shifting f ...

Nature Research2016

Extra short incubation microfluidic assisted – fluorescence in situ hybridization (ESIMA-FISH)

Martinus Gijs, Estelle Annick Cuttaz, Raphaël Etienne Jean Trouillon, Huu Tuan Nguyen, Laurène Nathalie Dupont

Fluorescence in situ hybridization (FISH) is a powerful technique for evaluating the HER2 gene status in breast cancer specimen (1). However, most of FISH assays currently used in clinical laboratories are expensive and require long experimental time, up t ...

2016

Track C. Biomedical Instrumentation & Micro and Nano Systems

Thomas Lehnert, Martinus Gijs, Johan Auwerx, Shreya Narasimhan, Matteo Cornaglia, Laurent Mouchiroud, Alexis Pierre Henri Marette, Virginija Jovaisaite, Govindan Narayanan Krishnamani, Vincenzo Sorrentino

De Gruyter2016

FLUORESCENCE IN SITU HYBRIDIZATION (FISH) ENHANCEMENT USING MICROFLUIDIC FLOW FOR AN ACCURATE, FAST AND ECONOMICAL ASSESSMENT OF HER2 STATUS IN BREAST CANCER

Martinus Gijs, Raphaël Etienne Jean Trouillon, Huu Tuan Nguyen, Maryse Fiche, Seiya Matsuoka

The fluorescence in situ hybridization (FISH) is the gold standard in human epidermal growth factor receptor 2 (HER2) status assessment in breast cancer. The dissemination of the technique is impeded by the cost of reagents and long experimental time. For ...

RCS Publising2016

Super-Resolution Imaging of a Dielectric Microsphere Is Governed by the Waist of Its Photonic Nanojet

Martinus Gijs, Hui Yang, Raphaël Etienne Jean Trouillon, Gergely Huszka

Dielectric microspheres with appropriate refractive index can image objects with super-resolution, that is, with a precision well beyond the classical diffraction limit. A microsphere is also known to generate upon illumination a photonic nanojet, which is ...

American Chemical Society2016

Dynamic electrochemical quantitation of dopamine release from a cells-on-paper system

Martinus Gijs, Raphaël Etienne Jean Trouillon

A hybrid microfluidic/electrochemical system is described for the time-resolved detection of dopamine (DA) from neuron-like PC12 cells cultured on a patch of filter paper. Cell adherence to the surface of the paper is investigated using fluorescence micros ...

Royal Soc Chemistry2016

Microfluidic device, system and method for the study of organisms

Martinus Gijs, Matteo Cornaglia, Daniel Migliozzi

The invention discloses a microfluidic device for the culture, selection and/or analysis of sample organisms such as nematodes, as well as for other biological entities such as for instance animal embryos. The device features reservoirs, culture chambers a ...

2016

Synergistic effect of carboxylic and amine ligands on the synthesis of CdSe nanocrystals

Martinus Gijs, Virendra Kumar Parashar

We demonstrate the drastic improvement in absorbance and photoluminescence (UV-PL) of CdSe nanocrystals (NCs) upon adding both a carboxylic acid and an amine ligand to the cadmium acetate green chemistry source. We combined carboxylate complexes of lauric ...

Royal Soc Chemistry2016

Automated longitudinal monitoring of in vivo protein aggregation in neurodegenerative disease C. elegans models

Thomas Lehnert, Martinus Gijs, Johan Auwerx, Matteo Cornaglia, Laurent Mouchiroud, Govindan Narayanan Krishnamani, Vincenzo Sorrentino

Background: While many biological studies can be performed on cell-based systems, the investigation of molecular pathways related to complex human dysfunctions - e.g. neurodegenerative diseases - often requires long-term studies in animal models. The nemat ...

BioMed Central2016

Understanding the mixing process in 3D microfluidic nozzle/diffuser systems: simulations and experiments

Abdeljalil Sayah, Martinus Gijs

We characterise computationally and experimentally a three-dimensional (3D) microfluidic passive mixer for various Reynolds numbers ranging from 1 to 100, corresponding to primary flow rates of 10-870 mu l min(-1). The 3D mixing channel is composed of mult ...

Iop Publishing Ltd2016

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