Publications de Thomas Lehnert

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In a typical high-throughput drug screening (HTS) process, up to millions of chemical compounds are applied to cells cultured in well plates, aiming to find molecules that exhibit a robust dose-response, as evidenced for example by a fluorescence signal. I ...

The roundworm Caenorhabditis elegans (C. elegans) is a powerful model organism for addressing fundamental biological questions related to human disease and aging. Its life cycle consists of an embryo stage, four larval stages that can be clearly distinguis ...

Microorganisms, encompassing both uni- and multicellular entities, exhibit remarkable diversity as omnipresent life forms in nature. They play a pivotal role by supplying essential components for sustaining biological processes across diverse ecosystems, i ...

Phenotypic diversity in bacterial flagella-induced motility leads to complex collective swimming patterns, appearing as traveling bands with transient locally enhanced cell densities. Traveling bands are known to be a bacterial chemotactic response to self ...

DNA fragmentation is an essential process in developing genetic sequencing strategies, genetic research, as well as for the diagnosis of diseases with a genetic signature like cancer. Efficient on-chip DNA fragmentation protocols would be beneficial to pro ...

Like other animals, C. elegans nematodes have the ability to socially interact and to communicate through exchange and sensing of small soluble signaling compounds that help them cope with complex environmental conditions. For the time being, worm biocommu ...

Magnetic micro-and nanoparticles ('magnetic beads') have been used to advantage in many microfluidic devices for sensitive antigen (Ag) detection. Today, assays that use as read-out of the signal the number count of immobilized beads on a surface for quant ...

The treatment of critical size peripheral nerve defects represents one of the most serious problems in neurosurgery. If the gap size exceeds a certain limit, healing can't be achieved. Connection mismatching may further reduce the clinical success. The pre ...

We present a new method for three-dimensional (3D) magnetic focusing of magnetic microparticles in a microfluidic system. On-chip magnetic particle manipulation in themicrochannel is achieved by a high-gradientmagnetic field generated by means of a microma ...

Caenorhabditis elegans(C. elegans) constitutes an important model organism for use in nutrition and aging studies. We report a novel method for studying the dynamics ofEscherichia coli(E. coli) bacterial transit through the worms' intestine. A microfluidic ...

We describe the development of a microcalorimetric platform for accurate measurement of the heat power generated by chemical/biochemical reactions in liquid volumes of a few 10 µL, which we used to measure the heat of mixing of 1-propanol in deionized water. The system allows fast overall stabilization to a setpoint temperature with a stability of ± 1 mK. The combination of an open-well sample reservoir with a commercial nanocalorimetric chip (Xensor Integration) makes the platform easy to use and very vers

The genome of the nematode Caenorhabditis elegans shows far-reaching similarities to that of humans. C. elegans is therefore considered as a powerful model organism in biological research related to humans, for instance for the study of human diseases. Suc ...

Assays focusing on emerging biological phenomena in an animal's life can be performed during embryogenesis. While the embryo of Caenorhabditis elegans has been extensively studied, its biomechanical properties are largely unknown. Here, we demonstrate that ...

We report on the magnetic actuation of superparamagnetic particles in a microfluidic channel. The formation of a highly confined and dynamic plug of particles extending over the cross section of the microchannel is enabled by superposing a static and a tim ...

The Caenorhabditis elegans embryo is a widely used model for the functional analysis of dynamic cellular processes, such as chromosome segregation, cytokinesis or lineage analysis. However, the conventional embryo preparation method that relies on manually ...

Studies of the real-time dynamics of embryonic development require a gentle embryo handling method, the possibility of long-term live imaging during the complete embryogenesis, as well as of parallelization providing a population's statistics, while keepin ...

A straightforward and general way for monitoring chemical reactions is via their thermal signature. Such approach requires however an experimental setup with a high thermal stability that simultaneously allows time-resolved heat detection with high sensiti ...

Caenorhabditiselegans (C. elegans) has gained importance as a model for studying host-microbiota interactions and bacterial infections related to human pathogens. Assessing the fate of ingested bacteria in the worm's intestine is therefore of great interes ...

Cellular respiration is a fundamental feature of metabolic activity and oxygen consumption can be considered as a reliable indicator of bacterial aerobic respiration, including for facultative anaerobic bacteria like E. coli. Addressing the emerging global ...

Fast spreading of antimicrobial resistance is now considered a major global health threat. New technologies are required, enabling rapid diagnostics of bacterial infection combined with fast antimicrobial susceptibility testing (AST) for evaluating the eff ...

Thomas Lehnert

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Microfluidic systems for high-throughput and high-content screening using the nematode Caenorhabditis elegans

Versatile size-dependent sorting of C-elegans nematodes and embryos using a tunable microfluidic filter structure

Microfluidic systems for infectious disease diagnostics

Effect of inoculum size and antibiotics on bacterial traveling bands in a thin microchannel defined by optical adhesive

Bubble-enhanced ultrasonic microfluidic chip for rapid DNA fragmentation

On-chip microfluidic biocommunication assay for studying male-induced demise in C. elegans hermaphrodites

Dose-response curve of a microfluidic magnetic bead-based surface coverage sandwich assay

Surface Microstructures on Planar Substrates and Textile Fibers Guide Neurite Outgrowth: A Scaffold Solution to Push Limits of Critical Nerve Defect Regeneration?

Three-Dimensional Magnetic Focusing of Superparamagnetic Beads for On-Chip Agglutination Assays

Anin vivomicrofluidic study of bacterial transit inC. elegansnematodes

A microcalorimetric platform for studying the heat produced by chemical reactions in microliter volumes

C. Elegans Immobilization Using Deformable Microfluidics For In Vivo Studies Of Early Embryogenesis And Intestinal Microbiota

Force microscopy of the Caenorhabditis elegans embryonic eggshell

Quadrupolar magnetic actuation of superparamagnetic particles for enhanced microfluidic perfusion

An Integrated Microfluidic Device For C-Elegans Early Embryogenesis Studies And Drug Assays

An automated microfluidic platform for C. elegans embryo arraying, phenotyping, and long-term live imaging

Nanocalorimetric platform for accurate thermochemical studies in microliter volumes

An In Vivo Microfluidic Study of Bacterial Load Dynamics and Absorption in the C. elegans Intestine

Antimicrobial susceptibility testing by measuring bacterial oxygen consumption on an integrated platform

Fast antimicrobial susceptibility testing onEscherichia coliby metabolic heat nanocalorimetry