Publications de Horst Pick | EPFL Graph Search

Publications associées (41)

Print-Light-Synthesis of Gold Thin Film Electrodes for Electrochemical Sensing

The one-step fabrication of gold films by inkjet printing of a gold precursor ink and its photochemical reduction by exposure to UV light is presented. Inkjet printing creates on a substrate with high control micrometer-thin reaction volumes in which upon ...

Wiley2023

Recombinant expression and functional characterization of the mouse olfactory receptor mOR256-17 in mammalian cells

Horst Arthur Vogel, Horst Pick, Luigino Grasso, Thamani Dahoun

Olfactory receptors (ORs) constitute the largest family of sensory membrane proteins in mammals. They play a key role within the olfactory system in recognizing and discriminating a nearly unlimited number of structurally diverse odorous molecules. The mol ...

2011

Dual activities of odorants on olfactory and nuclear hormone receptors

Horst Arthur Vogel, Horst Pick, Olivia Baud, Sylvain Etter

We have screened an odorant compound library and discovered molecules acting as chemical signals that specifically activate both G-protein-coupled olfactory receptors (ORs) on the cell surface of olfactory sensory neurons and the human nuclear estrogen rec ...

American Society for Biochemistry and Molecular Biology2009

Computational modeling of the olfactory receptor Olfr73 suggests a molecular basis for low potency of olfactory receptor-activating compounds

Horst Arthur Vogel, Horst Pick, Shuguang Yuan, Thamani Dahoun, Marc Brugarolas Campillos

The mammalian olfactory system uses hundreds of specialized G-protein-coupled olfactory receptors (ORs) to discriminate a nearly unlimited number of odorants. Cognate agonists of most ORs have not yet been identified and potential non-olfactory processes m ...

Springer2019

Immuno-affinity Amperometric Detection of Bacterial Infections

Hubert Girault, Andreas Stephan Lesch, Horst Pick, Michel Prudent, Milica Jovic, Yingdi Zhu

A combination of an immuno-affinity enrichment strategy and sensitive amperometric read-out was implemented in a point-of-care platform intended for bacterial infection analysis. Bacterial cells, selectively captured and enriched from complex matrices thro ...

Wiley-VCH Verlag GmbH2018

Tape-Stripping Electrochemical Detection of Melanoma

Hubert Girault, Andreas Stephan Lesch, Horst Pick, Tzu-En Lin, Yingdi Zhu, Sorour Darvishi

A noninvasive electrochemical melanoma detection approach based on using adhesive tapes for collecting and fixing cells from a suspicious skin area and transferring the cells into a scanning electrochemical microscope (SECM) is presented. The adhesive laye ...

AMER CHEMICAL SOC2019

Sensitive and fast identification of bacteria in blood samples by immunoaffinity mass spectrometry for quick BSI diagnosis

Hubert Girault, Horst Pick, Liang Qiao, Natalia Gasilova, Alexandra Bondarenko, Niels Erik Lion, Michel Prudent, Yingdi Zhu

Bloodstream infections rank among the most serious causes of morbidity and mortality in hospitalized patients, partly due to the long period (up to one week) required for clinical diagnosis. In this work, we have developed a sensitive method to quickly and ...

Royal Soc Chemistry2016

Visualizing odorant receptor trafficking in living cells down to the single-molecule level

Horst Arthur Vogel, Horst Pick, Jean-Manuel Segura, Valérie Jacquier

Despite the importance of trafficking for regulating G protein-coupled receptor signaling, for many members of the seven transmembrane helix protein family, such as odorant receptors, little is known about this process in live cells. Here, the complete lif ...

National Academy of Sciences2006

Detection of bacteria

Hubert Girault, Andreas Stephan Lesch, Horst Pick, Milica Jovic, Yingdi Zhu

A method for bacteria detection and the assessment of the antimicrobial susceptibility profile uses a bacterial viability assay based on combining a highly efficient affinity bacteria enrichment/ separation with an electrochemical redox interrogation measu ...

2019

Soft Electrochemical Probes for Mapping the Distribution of Biomarkers and Injected Nanomaterials in Animal and Human Tissues

Hubert Girault, Andreas Stephan Lesch, Horst Pick, Tzu-En Lin, Yu Lu

Monitoring biomarkers and injected theranostic nanomaterials in tissues and organs plays a pivotal role in numerous medical applications ranging from cancer diagnostics to drug delivery. Scanning electrochemical microscopy has been demonstrated as a powerf ...

Wiley-Blackwell2017

MALDI Detection of Exosomes: A Potential Tool for Cancer Studies

Hubert Girault, Horst Pick, Natalia Gasilova, Tzu-En Lin, Yingdi Zhu

Matrix-assisted laser desorption ionization time-of-flight mass spectrometry is used to detect intact whole exosomes, yielding exosomal fingerprints within minutes. This rapid exosome detection approach is proposed as a potential tool for cancer studies. M ...

2019

Single-Vesicle Assays Using Liposomes and Cell-Derived Vesicles: From Modeling Complex Membrane Processes to Synthetic Biology and Biomedical Applications

Ana Catarina Rodrigues Alves, Horst Arthur Vogel, Horst Pick

The plasma membrane is of central importance for defining the closed volume of cells in contradistinction to the extracellular environment. The plasma membrane not only serves as a boundary, but it also mediates the exchange of physical and chemical inform ...

AMER CHEMICAL SOC2018

Diffusion-Time Distribution Analysis Reveals Characteristic Ligand-Dependent Interaction Patterns of Nuclear Receptors in Living Cells

Horst Arthur Vogel, Horst Pick, Hanna Jankevics, Paulina Izewska

Nuclear receptors initiate transcription, interact with regulatory proteins, and are influenced by hormones, drugs, and pollutants. Herein, we discover ligand-specific mobility patterns of human estrogen receptor-a (ER) in living cells using diffusion-time ...

2005

The Mouse Eugenol Odorant Receptor: Structural and Functional Plasticity of a Broadly Tuned Odorant Binding Pocket

Horst Arthur Vogel, Horst Pick, Olivia Baud, Sylvain Etter

Molecular interactions of odorants with their olfactory receptors (ORs) are of central importance for the ability of the mammalian olfactory system to detect and discriminate a vast variety of odors with a limited set of receptors. How a particular OR bind ...

American Chemical Society2011

Ligand binding transmits conformational changes across the membrane-spanning region to the intracellular side of the 5-HT3 serotonin receptor

Horst Arthur Vogel, Horst Pick, Cédric Deluz, Erwin Ilegems

Conformational changes of channel activation: Five enhanced green fluorescent protein (EGFP) mols. (green cylinders) were integrated into the intracellular part of the homopentameric ionotropic 5-HT3 receptor. This allowed the detection of extracellular bi ...

2005

Highly Loaded Mildly Edge‐Oxidized Graphene Nanosheet Dispersions for Large‐Scale Inkjet Printing of Electrochemical Sensors

Hubert Girault, Andreas Stephan Lesch, Horst Pick, Milica Jovic, Victor Costa Bassetto, Yingdi Zhu, Bhawna Nagar

Inkjet printing of electrochemical sensors using a highly loaded mildly edge‐oxidized graphene nanosheet (EOGN) ink is presented. An ink with 30 mg/mL EOGNs is formulated in a mixture of N‐methyl pyrrolidone and propylene glycol with only 30 min of sonicat ...

2020

Effective removal of the rotifer Brachionus calyciflorus from a Chlorella vulgaris microalgal culture by homogeneous solar photo-Fenton at neutral pH

Christian Ludwig, Stefanos Giannakis, Horst Pick, Adrian Pulgarin

In this study, a citrate-modified photo-Fenton process was successfully applied to decontaminate a Chlorella vulgaris microalgae culture spiked with the rotifer Brachionus calyciflorus (5 individuals mL−1). The applied treatment (1 mg L−1 Fe2+, 20 mg L−1 H ...

2022

Purification of Cellulose and Chitin Polymers and Other Value-Added Products from the Microalga Chlorella vulgaris Using a Green Biorefinery Process

Maxime Frabboni, Christian Ludwig, Horst Pick, Eya Nicodeme, Enio Daniel Zanchetta

Microalgae produce renewable biomass at high efficiency by using photosynthesis to capture the greenhouse gas CO2 from the atmosphere and releasing O2. Chlorella vulgaris is one of the most frequently cultivated unicellular green microalgae of commercial r ...

2025

Monitoring proliferative activities of hormone-like odorants in human breast cancer cells by gene transcription profiling and electrical impedance spectroscopy

Horst Arthur Vogel, Cathrin Brisken, Horst Pick, Samuel Terrettaz, Olivia Baud

The human estrogen receptor alpha (ER!) mediates the proliferative action of hormones in breast cancer cells by regulating the expression of target genes to control cellular functions. Current methodologies do not permit a real-time assessment of these pro ...

Elsevier2013

Investigating cellular signaling reactions in single attoliter vesicles

Horst Arthur Vogel, Horst Pick, Rudolf Hovius, Erwin Ilegems

Understanding cellular signaling mediated by cell surface receptors is key to modern biomedical research and drug development. The discovery of a growing no. of potential mol. targets and therapeutic compds. requires downscaling and accelerated functional ...

2005