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Matthias Lütolf

Cette personne a quitté l’EPFL

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Publications associées (176)

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Label-Free Quantification Proteomics for the Identification of Mesenchymal Stromal Cell Matrisome Inside 3D Poly(Ethylene Glycol) Hydrogels

Matthias Lütolf, Yannick Robert Devaud

Cells modulate the functional properties of their environment by depositing extracellular matrix (ECM) proteins during biological processes in vivo and in vitro. Despite the ECMs central role in tissue formation, its quantification in hydrogels like Matrig ...

WILEY2018

3D niche microarrays for systems-level analyses of cell fate

Matthias Lütolf, Katarzyna Mosiewicz, Adrian Ranga, Samy Gobaa, Andrea Negro, Yuya Okawa

The behaviour of mammalian cells in a tissue is governed by the three-dimensional (3D) microenvironment and involves a dynamic interplay between biochemical and mechanical signals provided by the extracellular matrix (ECM), cell–cell interactions and solub ...

Nature Publishing Group2014

Engineering organoids

Matthias Lütolf, Moritz Hofer

Organoids are in vitro miniaturized and simplified model systems of organs that have gained enormous interest for modelling tissue development and disease, and for personalized medicine, drug screening and cell therapy. Despite considerable success in cult ...

NATURE RESEARCH2021

Gastruloids generated without exogenous Wnt activation develop anterior neural tissues

Matthias Lütolf, Mehmet Ugur Girgin, Nicolas Antoine Vincent Broguiere

When stimulated with a pulse from an exogenous WNT pathway activator, small aggregates of mouse embryonic stem cells (ESCs) can undergo embryo-like axial morphogenesis and patterning along the three major body axes. However, these structures, called gastru ...

2021

Bioengineering approaches to guide stem cell-based organogenesis

Matthias Lütolf, Adrian Ranga, Nikolche Gjorevski

During organogenesis, various molecular and physical signals are orchestrated in space and time to sculpt multiple cell types into functional tissues and organs. The complex and dynamic nature of the process has hindered studies aimed at delineating morpho ...

Company of Biologists2014

Capturing Complex Protein Gradients on Biomimetic Hydrogels for Cell-Based Assays

Matthias Lütolf, Steffen Cosson, Stefan Kobel

versatile strategy to rapidly immobilize complex gradients of virtually any desired protein on soft poly(ethylene glycol) (PEG) hydrogel surfaces that are reminiscent of natural extracellular matrices (ECM) is reported. A microfluidic chip is used to gener ...

2009

The NAD-Booster Nicotinamide Riboside Potently Stimulates Hematopoiesis through Increased Mitochondrial Clearance

Matthias Lütolf, Vincent Trachsel, Bart Deplancke, Nicola Vannini, Johan Auwerx, Pedro Romero, Carlos Canto Alvarez, Mukul Girotra, Aurélien Laurent Jean-Charles Oggier, Dongryeol Ryu, Eija Riitta Pirinen, Olaia Maria Naveiras Torres-Quiroga, Pernille Yde Rainer, Gennady Nikitin, Vasco Ledebur de Antas de Campos, Joanna Ratajczak, Josefine Catharina Pedersen Tratwal, George Coukos, Sonja Giger, Shanti Rojas-Sutterlin, Yang Li

It has been recently shown that increased oxidative phosphorylation, as reflected by increased mitochondrial activity, together with impairment of the mitochondrial stress response, can severely compromise hematopoietic stem cell (HSC) regeneration. Here w ...

CELL PRESS2019

Artificial stem cell niches

Matthias Lütolf

Stem cells are characterized by their dual ability to reproduce themselves (self-renew) and specialize (differentiate), yielding essentially unlimited numbers of daughter cells that can maintain and regenerate tissues. In contrast to their embryonic counte ...

Wiley2009

Developmental dynamics of the neural crest-mesenchymal axis in creating the thymic microenvironment

Matthias Lütolf, Tania Hübscher

The thymic stroma is composed of epithelial and nonepithelial cells providing separate microenvironments controlling homing, differentiation, and selection of hematopoietic precursor cells to functional T cells. Here, we explore at single-cell resolution t ...

AMER ASSOC ADVANCEMENT SCIENCE2022

Artificial niche microarrays for probing single stem cell fate in high throughput

Matthias Lütolf, Stefan Kobel, Samy Gobaa, Andrea Negro

To understand the regulatory role of niches in maintaining stem-cell fate, multifactorial in vitro models are required. These systems should enable analysis of biochemical and biophysical niche effectors in a combinatorial fashion and in the context of a p ...

Nature Publishing Group2011

Pharmacological induction of a progenitor state for the efficient expansion of primary human hepatocytes

Matthias Lütolf, Didier Trono, Nathalie Brandenberg, Marc Samuel Friedli, Marco Cassano, Carmen Maria Unzu Ezquerro, Evaristo Jose Planet Letschert

The liver is an organ with strong regenerative capacity, yet primary hepatocytes have a low amplification potential in vitro, a major limitation for the cell-based therapy of liver disorders and for ex vivo biological screens. Induced pluripotent stem cell ...

2019

Gastruloids as in vitro models of embryonic blood development with spatial and temporal resolution

Matthias Lütolf, Tania Hübscher, Sonja Giger, Giuliana Rossi

Gastruloids are three-dimensional embryonic organoids that reproduce key features of early mammalian development in vitro with unique scalability, accessibility, and spatiotemporal similarity to real embryos. Recently, we adapted the gastruloid culture con ...

Nature Portfolio2022

Integration column: microwell arrays for mammalian cell culture

Matthias Lütolf

Microwell arrays have emerged as robust and versatile alternatives to conventional mammalian cell culture substrates. Using standard microfabrication processes, biomaterials surfaces can be topographically patterned to comprise high-density arrays of micro ...

2009

High-throughput approaches for the analysis of extrinsic regulators of stem cell fate

Matthias Lütolf, Adrian Ranga

The complexity of stem cell niches poses a tremendous challenge to understanding mechanisms of extrinsic regulation of stem cell fate. In order to better understand niche signaling and its effect on stem cell fate choices, in vitro systems are being engine ...

2012

Capturing Cell-Cell Interactions via SNAP-tag and CLIP-tag Technology

Matthias Lütolf

Juxtacrine or contact-dependent signaling is a major form of cell communication in multicellular organisms. The involved cell-cell and cell-extracellular-matrix (ECM) interactions are crucial for the organization and maintenance of tissue architecture and ...

Amer Chemical Soc2015

Synthetic biomaterials as instructive extracellular microenvironments for morphogenesis in tissue engineering

Matthias Lütolf, Jeffrey Alan Hubbell

A review. New generations of synthetic biomaterials are being developed at a rapid pace for use as three-dimensional extracellular microenvironments to mimic the regulatory characteristics of natural extracellular matrixes (ECMs) and ECM-bound growth facto ...

2005

Part II: Fibroblasts preferentially migrate in the direction of principal strain

Matthias Lütolf, Jeffrey Alan Hubbell

A growing body of evidence suggests that the sensory information from the cytoskeleton and integrins may be responsible for guiding migration during mechano- and haptotaxis. However, the dual function of these subcellular structures as mechano-sensors and ...

Springer-Verlag2008

Progress and potential in organoid research

Matthias Lütolf, Andrea Manfrin, Giuliana Rossi

Tissue and organ biology are very challenging to study in mammals, and progress can be hindered, particularly in humans, by sample accessibility and ethical concerns. However, advances in stem cell culture have made it possible to derive in vitro 3D tissue ...

2018

Thymic epithelial organoids mediate T-cell development

Matthias Lütolf, Tania Hübscher, Jakob Josef Langer, Lucie Carrié Tillard

Although the advent of organoids has opened unprecedented perspectives for basic and translational research, immune system-related organoids remain largely underdeveloped. Here, we established organoids from the thymus, the lymphoid organ responsible for T ...

2024

High-throughput clonal analysis of neural stem cells in microarrayed artificial niches

Matthias Lütolf, Samy Gobaa

To better understand the extrinsic signals that control neural stem cell (NSC) fate, here we applied a microwell array platform which allows high-throughput clonal analyses of NSCs, cultured either as neurospheres or as adherent clones, exposed to poly(eth ...

2012