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Christian Depeursinge

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Publications associées (88)

Cell Morphology and Intracellular Ionic Homeostasis explored with a Multimodal Approach combining Epifluorescence and Digital Holographic Microscopy

Christian Depeursinge, Pierre Marquet, Jonas Kühn, Pierre Magistretti, Nicolas Pavillon, Pascal Jourdain, Daniel Boss, Corinne Moratal, Aleksandr Benke

The authors have developed a live-cell multimodality microscope combining epifluorescence with digital holographic microscopy; it has been implemented with a decoupling procedure allowing to separately measure from the quantitative phase important cell par ...

2010

Signification of the phase function parameter gamma explored with a fractal distribution of scatterers

Stéphane Chamot, Christian Depeursinge, Pierre Marquet, Elena Migacheva

The optical properties within limited volumes of diffusive media can be probed by carrying spatially-resolved measurements of diffused light at short source-detector separation (typically one scattering mean free path). At such distance, analytical models ...

Optical Society of America2010

Label-free second harmonic phase imaging of biological specimen by digital holographic microscopy

Christian Depeursinge, Pierre Marquet, Pierre Magistretti, Corinne Moratal, Etienne Shaffer

We have previously developed a new way for nonscanning second-harmonic generation (SHG) microscopy [Opt. Lett. 34, 2450 (2009)]. Based on digital holography, this technique captures, in single-shot hologram acquisition, both the amplitude and the phase of ...

Optical Society of America2010

Iterative method for zero-order suppression in off-axis digital holography

Christian Depeursinge, Nicolas Pavillon, Cristian Arfire

We propose a method to suppress the so-called zero-order term in a hologram, based on an iterative principle. During the hologram acquisition process, the encoded information includes the intensities of the two beams creating the interference pattern, whic ...

Optical Society of America2010

Extended depth-of-focus by digital holographic microscopy

Tristan Colomb, Etienne Cuche, Christian Depeursinge, Jonas Kühn, Nicolas Pavillon

A recurrent problem in microscopy is the finite depth-of-focus linked to the NA of microscope objectives. Digital holographic microscopy (DHM) has the unique feature of being able to numerically change the focus from a single hologram without the need of m ...

Optical Society of America2010

Zero-Order-Free Image Reconstruction in Digital Holographic Microscopy

Christian Depeursinge, Michaël Unser, Nicolas Pavillon, Chandra Sekhar Seelamantula

We address the problem of zero-order-free image reconstruction in digital holographic microscopy. We show how the goal can be achieved by confining the object-wave modulation to one quadrant of the frequency domain, and by maintaining a reference-wave inte ...

IEEE2009

A Nonlinear Technique for Automatic Twin-Image and Zero-Order Term Suppression in Digital Holographic Microscopy

Christian Depeursinge, Michaël Unser, Nicolas Pavillon, Chandra Sekhar Seelamantula

ITO2009

Off-axis low coherence interferometry contouring

Christian Depeursinge, Yves Delacrétaz, Nicolas Pavillon, Florian Lang

In this article we present a method to achieve tri-dimensional contouring of macroscopic objects. A modified reference wave speckle interferometer is used in conjunction with a source of reduced coherence. The depth signal is given by the envelope of the i ...

2009

Red blood cell structure and dynamics explored with digital holographic microscopy

Christian Depeursinge, Pierre Marquet, Pierre Magistretti, Pascal Jourdain, Daniel Boss, Benjamin Rappaz

2009

Cellular Dynamics Revealed by Digital Holographic Microscopy

Christian Depeursinge

Digital holographic microscopy (DHM) is a new optical method taking advantage of coherent properties of light propagation and it provides, without the use of any contrast agent, real-time three-dimensional images of transparent living cells, with an axial ...

Elsevier2009

Spatial analysis of erythrocyte membrane fluctuations by digital holographic microscopy

Christian Depeursinge, Pierre Marquet, Pierre Magistretti, Annick Hoffmann, Daniel Boss, Benjamin Rappaz

Red blood cell (RBC) membrane fluctuations provide important insights into cell states. We present a spatial analysis of red blood cell membrane fluctuations by using digital holographic Microscopy (DHM). This interferometric and dye-free technique, posses ...

Elsevier2009

Optical recording of cell electrical activity by Digital Holographic Microscopy

Christian Depeursinge, Pierre Marquet, Pierre Magistretti, Pascal Jourdain, Daniel Boss, Benjamin Rappaz

2009

Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy

Tristan Colomb, Christian Depeursinge, Pierre Marquet, Jonas Kühn, Pierre Magistretti, Benjamin Rappaz, Viesturs Simanis, Elena Cano

Digital holography microscopy (DHM) is an optical technique which provides phase images yielding quantitative information about cell structure and cellular dynamics. Furthermore, the quantitative phase images allow the derivation of other parameters, inclu ...

SPIE Society of Photo-optical Instrumentation Engineers2009

Submicrometer tomography of cells by multiple-wavelength digital holographic microscopy in reflection

Tristan Colomb, Frédéric Montfort, Christian Depeursinge, Pierre Marquet, Jonas Kühn, Nicolas Pavillon, Benjamin Rappaz, Corinne Moratal

We present first results on a method enabling mechanical scanning-free tomography with submicrometer axial resolution by multiple-wavelength digital holographic microscopy. By sequentially acquiring reflection holograms and summing 20 wavefronts equally sp ...

2009

Digital holographic microscopy investigation of second harmonic generated at a glass/air interface

Christian Depeursinge, Jonas Kühn, Nicolas Pavillon, Etienne Shaffer

Optical second harmonic generation, thanks to its coherent nature, is a suitable signal for interferometric measurements, such as digital holography: a well-established imaging technique that allows recovering complex diffraction wavefields from which it i ...

2009

Suppression of the zero-order in off-axis digital holography through nonlinear filtering

Christian Depeursinge, Michaël Unser, Jonas Kühn, Nicolas Pavillon, Chandra Sekhar Seelamantula

We present experimental validation of a new reconstruction method for off-axis digital holographic microscopy (DHM). This method effectively suppresses the object auto-correlation — namely the zero-order term — from holographic data, thereby improving the ...

2009

Method and apparatus to measure and compute the amplitude point spread function and associated parameters of a coherent optical imaging system

Tristan Colomb, Christian Depeursinge, Pierre Marquet, Florian Charrière, Mihaela Marian

The invention concerns a method of and an apparatus for the measurement of the Amplitude Point Spread Function (APSF), of an Optical Imaging System (OIS), wherein the APSF is defined as the complex field distribution of the beam delivered by the OIS and or ...

2008

Simultaneous cell morphometry and refractive index measurement with dual- wavelength digital holographic microscopy and dye-enhanced dispersion of perfusion medium

Christian Depeursinge, Pierre Marquet, Florian Charrière, Pierre Magistretti, Benjamin Rappaz

Digital holographic microscopy (DHM) allows optical-path-difference (OPD) measurements with nanometric accuracy. OPD induced by transparent cells depends on both the refractive index (RI) of cells and their morphology. This Letter presents a dual-wavelengt ...

2008

Comparative study of human erythrocytes by digital holographic microscopy, confocal microscopy, and impedance volume analyzer

Christian Depeursinge, Pierre Marquet, Pierre Magistretti, Benjamin Rappaz

Red blood cell (RBC) parameters such as morphology, volume, refractive index, and hemoglobin content are of great importance for diagnostic purposes. Existing approaches require complicated calibration procedures and robust cell perturbation. As a result, ...

2008

Axial sub-nanometer accuracy in digital holographic microscopy

Tristan Colomb, Frédéric Montfort, Etienne Cuche, Christian Depeursinge, Pierre Marquet, Jonas Kühn, Florian Charrière

2008

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