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Christian Depeursinge

Cette personne a quitté l’EPFL

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Publications associées (87)

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Amplitude point spread function measurement of high NA microscope objectives by digital holographic microscopy

Pierre Marquet, Christian Depeursinge, Tristan Colomb, Florian Charrière, Mihaela Marian

We present here a three-dimensional evaluation of the amplitude point-spread function (APSF) of a microscope objective (MO), based on a single holographic acquisition of its pupil wavefront. The aberration function is extracted from this pupil measurements ...

2007

Monte Carlo simulation of the field back-scattered from rough surfaces

Christian Depeursinge, Stéphane Chamot

A novel approach for the simulation of the field back-scattered from a rough surface is presented. It takes into account polarization and multiple scattering events on the surface, as well as diffraction effects. The validity and usefulness of this simulat ...

2012

Method for measuring locally and superficially the scattering and absorption properties of turbid media

Christian Depeursinge

We present a method and apparatus for local and superficial measurement of the optical properties of turbid media. The depth probed is on the order of 1 transport mean free path of the photon. The absorption coefficient, reduced scattering coefficient and ...

2005

Comparative study of human erythrocytes by digital holographic microscopy, confocal microscopy, and impedance volume analyzer

Pierre Magistretti, Pierre Marquet, Christian Depeursinge, Benjamin Rappaz

Red blood cell (RBC) parameters such as morphology, volume, refractive index, and hemoglobin content are of great importance for diagnostic purposes. Existing approaches require complicated calibration procedures and robust cell perturbation. As a result, ...

2008

Apparatus and method for quantitative phase tomography through linear scanning with coherent and non-coherent detection

Christian Depeursinge, Nicolas Pavillon

The disclosed invention describes a new apparatus performing a new data acquisition for quantitative refractive index tomography. It is based on a linear scanning of the specimen, opposed to the classical approaches based on rotations of either the sample ...

2013

A Nonlinear Technique for Automatic Twin-Image and Zero-Order Term Suppression in Digital Holographic Microscopy

Michaël Unser, Christian Depeursinge, Nicolas Pavillon, Chandra Sekhar Seelamantula

ITO2009

Single-shot, simultaneous incoherent and holographic microscopy

Christian Depeursinge, Nicolas Pavillon, Etienne Shaffer

In this work, we demonstrate single-shot, simultaneous recording and subsequent retrieval of one incoherent and two holographic (intensity and phase) images from the same camera frame. Demultiplexing of incoherent and holographic signals in the spatial fre ...

Wiley-Blackwell2012

Off-axis low coherence interferometry contouring

Christian Depeursinge, Nicolas Pavillon, Florian Lang

In this article we present a method to achieve tri-dimensional contouring of macroscopic objects. A modified reference wave speckle interferometer is used in conjunction with a source of reduced coherence. The depth signal is given by the envelope of the i ...

2009

Method and apparatus for retrieval of amplitude and phase of nonlinear electromagnetic waves

Christian Depeursinge, Etienne Shaffer

The present invention discloses a method and its associated apparatus to retrieve the amplitude and, especially, the phase of nonlinear electromagnetic waves. The application field of the present invention is optical imaging. A sample is probed by coherent ...

2010

Exploring Neural Cell Dynamics with Digital Holographic Microscopy

Pierre Magistretti, Pierre Marquet, Christian Depeursinge

In this review, we summarize how the new concept of digital optics applied to the field of holographic microscopy has allowed the development of a reliable and flexible digital holographic quantitative phase microscopy (DH-QPM) technique at the nanoscale p ...

Annual Reviews2013

Real-time dual-wavelength digital holographic microscopy with a single hologram acquisition

Pierre Marquet, Christian Depeursinge, Jonas Kühn, Tristan Colomb, Etienne Cuche, Florian Charrière, Frédéric Montfort

A technique to perform two-wavelengths digital holographic microscopy (DHM) measurements with a single hologram acquisition is presented. The vertical measurement range without phase ambiguity is extended to the micron-range, thanks to the resulting synthe ...

2007

Early Cell Death Detection with Digital Holographic Microscopy

Pierre Magistretti, Pierre Marquet, Christian Depeursinge, Nicolas Pavillon, Pascal Jourdain

Background: Digital holography provides a non-invasive measurement of the quantitative phase shifts induced by cells in culture, which can be related to cell volume changes. It has been shown previously that regulation of cell volume, in particular as it r ...

Public Library of Science2012

Shot noise influence in reconstructed phase images SNR in digital holographic microscopy

Pierre Marquet, Christian Depeursinge, Etienne Cuche, Florian Charrière

We present a study based on simulations describing how shot noise, an intrinsic part of the recording process with a digital camera, influences the quality of the reconstructed phase images under different beams intensities configurations. ...

SPIE2005

Living specimen tomography by digital holographic microscopy: morphometry of testate amoeba

Pierre Marquet, Christian Depeursinge, Tristan Colomb, Nicolas Pavillon, Benjamin Rappaz, Florian Charrière

This paper presents an optical diffraction tomography technique based on digital holographic microscopy. Quantitative 2-dimensional phase images are acquired for regularly-spaced angular positions of the specimen covering a total angle of π, allowing to bu ...

2006

Dual wavelength full field imaging in low coherence digital holographic microscopy

Christian Depeursinge, Christophe Moser, Frédéric Montfort, Zahra Monemhaghdoust

A diffractive optical element (DOE) is presented to simultaneously manipulate the coherence plane tilt of a beam containing a plurality of discrete wavelengths. The DOE is inserted into the reference arm of an off-axis dual wavelength low coherence digital ...

Optical Society of America2011

Label-Free Cytotoxicity Screening Assay by Digital Holographic Microscopy

Pierre Magistretti, Christian Depeursinge, Gerardo Turcatti, Benjamin Rappaz, Jonas Kühn, Marc Chambon, Etienne Shaffer, Jérôme Parent, Julien Mena

We introduce a label-free technology based on digital holographic microscopy (DHM) with applicability for screening by imaging, and we demonstrate its capability for cytotoxicity assessment using mammalian living cells. For this first high content screenin ...

Mary Ann Liebert2013

Digital holographic microscopy applied to life sciences

Pierre Marquet, Christian Depeursinge, Jonas Kühn, Tristan Colomb, Benjamin Rappaz, Florian Charrière

Digital holographic microscopy (DHM) is applied to life sciences applications and demonstrate its capability of real-time imaging and quantitative measurements of physiological parameters such as cell volume or mean cell hemoglobin concentration (MCHC) of ...

IEEE2007

Quantitative phase restoration by direct inversion using the optical transfer function

Pierre Marquet, Christian Depeursinge

Quantitative phase recovery of phase objects is achieved by a direct inversion using the defocused weak object transfer function. The presented method is noniterative and is based on partially coherent principles. It also takes into account the optical pro ...

Optical Society of America2011

A Practical Inverse-Problem Approach to Digital Holographic Reconstruction

Michaël Unser, Christian Depeursinge, Nicolas Pavillon, Emrah Bostan, Aurélien Bourquard

In this paper, we propose a new technique for high-quality reconstruction from single digital holographic acquisitions. The unknown complex object field is found as the solution of a nonlinear inverse problem that consists in the minimization of an energy ...

OSA2013

Accelerated autofocusing of off-axis holograms using critical sampling

Christian Depeursinge, Muhammed Fatih Toy, Jérôme Parent

In this Letter we propose a fast off-axis hologram autofocusing (AF) approach that is based on the redundant data elimination by the critical resampling of the contained complex field. Implementation of the proposed methodology enables the real-time AF wit ...

Optical Society of America2012