Publications de Jean-Baptiste Perez

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G Protein coupled receptors (GPCRs) constitute an abundant family of membrane proteins which play a central role in cellular signaling and are important targets for modern medicine. Despite intensive research, central questions of the molecular mechanism o ...

Cell-membrane sheets suitable for in-vitro functional fluorescence studies have been prepd. by direct detachment from cell membranes using poly-L-lysine-coated glass slides. The resulting transferred planar membranes conserve the compn. as well as most pro ...

How membrane receptors transduce external signals across the membrane and activate intracellular processes is a key question for fundamental research and for therapeutic purposes. Reliable in situ investigations requiest to preserve the full biological activity, the organization and the lateral mobility of proteins in the lipid membrane. Here we demonstrate the immobilization of native cell membranes on microstructured devices consisting of an array of 200 nm holes in a free standing planar SiN support. Thi

We combined biophys., biochem., and pharmacol. approaches to investigate the ability of the a1a- and a1b-adrenergic receptor (AR) subtypes to form homo- and hetero-oligomers. Receptors tagged with different epitopes (hemagglutinin and Myc) or fluorescent proteins (cyan and green fluorescent proteins) were transiently expressed in HEK-293 cells either individually or in different combinations. Fluorescence resonance energy transfer measurements provided evidence that both the a1a- and a1b-AR can form homo-ol

Supported cell-membrane sheets are promising in vitro systems to investigate the properties of membranes with native protein/lipid composition, in particular their sub-compartmentalization and the differential localization of proteins associated to them. W ...

Cell membrane receptors and ion channels are essential in many different cellular processes. To analyse the activity of membrane proteins in vesicles and biological cells, we fabricated micro- nanostructured chips, enabling the application of electrophysio ...

The authors present a method for spreading large (> 100 mm2) cell membrane fragments across nanoapertures in planar supports. Electron-beam and focused-ion-beam lithog. were used to fabricate arrays of 50-600 nm diam. holes in free-standing silicon nitride ...

Jean-Baptiste Perez

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Investigating molecular interactions of G protein coupled receptors by fluorescence techniques

Supported cell-membrane sheets for functional fluorescence imaging of membrane proteins

Native membranes on subwavelentgh holes for membrane receptors analysis

Oligomerization of the alpha 1a- and alpha 1b-Adrenergic Receptor Subtypes: Potential Implications in Receptor Internalization

Monitoring the Diffusion of Single Heterotrimeric G Proteins in Supported Cell-membrane Sheets Reveals their Partitioning into Microdomains

Micro- and nanostructured devices for investigating biomolecular interactions

Cell Membranes Suspended Across Nanoaperture Arrays