Publications by Jean-Baptiste Perez

Related publications (5)

Investigating molecular interactions of G protein coupled receptors by fluorescence techniques

G Protein coupled receptors (GPCRs) constitute an abundant family of membrane proteins which play a central role in cellular signaling and are important targets for modern medicine. Despite intensive research, central questions of the molecular mechanism o ...

EPFL2005

Supported cell-membrane sheets for functional fluorescence imaging of membrane proteins

Horst Arthur Vogel, Jean-Manuel Segura, Jean-Baptiste Perez

Cell-membrane sheets suitable for in-vitro functional fluorescence studies have been prepd. by direct detachment from cell membranes using poly-L-lysine-coated glass slides. The resulting transferred planar membranes conserve the compn. as well as most pro ...

2006

Monitoring the Diffusion of Single Heterotrimeric G Proteins in Supported Cell-membrane Sheets Reveals their Partitioning into Microdomains

Horst Arthur Vogel, Jean-Manuel Segura, Joachim Piguet, Jean-Baptiste Perez

Supported cell-membrane sheets are promising in vitro systems to investigate the properties of membranes with native protein/lipid composition, in particular their sub-compartmentalization and the differential localization of proteins associated to them. W ...

Elsevier2006

Micro- and nanostructured devices for investigating biomolecular interactions

Jürgen Brugger, Horst Arthur Vogel, Christian Santschi, Christophe Danelon, Christoph Schreiter, Jean-Baptiste Perez

Cell membrane receptors and ion channels are essential in many different cellular processes. To analyse the activity of membrane proteins in vesicles and biological cells, we fabricated micro- nanostructured chips, enabling the application of electrophysio ...

2006

Cell Membranes Suspended Across Nanoaperture Arrays

Jürgen Brugger, Horst Arthur Vogel, Christian Santschi, Christophe Danelon, Jean-Baptiste Perez

The authors present a method for spreading large (> 100 mm2) cell membrane fragments across nanoapertures in planar supports. Electron-beam and focused-ion-beam lithog. were used to fabricate arrays of 50-600 nm diam. holes in free-standing silicon nitride ...

2006