Concept

Membrane estrogen receptor

Publications associées (29)

Bioconjugated lanthanide luminescent helicates as multilabels for lab-on-a-chip detection of cancer biomarkers

Martinus Adela Maria Gijs, Jean-Claude Bünzli, Caroline Vandevyver, Anne-Sophie Chauvin, Venkataragavalu Sivagnanam, Bo Song, Hans-Anton Lehr

The lanthanide binuclear helicate [Eu2(LC2(CO2H))3] is coupled to avidin to yield a luminescent bioconjugate EuB1 (Q ¼ 9.3%, s(5D0) ¼ 2.17 ms). MALDI/TOF mass spectrometry confirms the covalent binding of the Eu chelate and UV-visible spectroscopy allows o ...

2010

The orphan nuclear receptor small heterodimer partner mediates male infertility induced by diethylstilbestrol in mice

Johan Auwerx, Kristina Schoonjans

Studies in rodents have shown that male sexual function can be disrupted by fetal or neonatal administration of compounds that alter endocrine homeostasis, such as the synthetic nonsteroidal estrogen diethylstilbestrol (DES). Although the molecular basis f ...

American Society for Clinical Investigation2009

Relationship between estrogen receptor alpha location and gene induction reveals the importance of downstream sites and cofactors

Felix Naef, Mauro Delorenzi

BACKGROUND: To understand cancer-related modifications to transcriptional programs requires detailed knowledge about the activation of signal-transduction pathways and gene expression programs. To investigate the mechanisms of target gene regulation by hum ...

BioMed Central2009

Miniaturized bioanalytics to probe the function of membrane proteins

G-protein coupled receptors (GPCRs) are the most abundant class of proteins in the cell body. Such receptors are of major interest as potential therapeutic targets. Downscaling and parallelization of bioanalytics opens novel routes to rapidly screen and id ...

EPFL2008

The Ret receptor tyrosine kinase pathway functionally interacts with the ERalpha pathway in breast cancer

Cathrin Brisken

A limited number of receptor tyrosine kinases (e.g., ErbB and fibroblast growth factor receptor families) have been genetically linked to breast cancer development. Here, we investigated the contribution of the Ret receptor tyrosine kinase to breast tumor ...

2008

Method for testing a substance interacting with a target molecule

Horst Arthur Vogel, Hanna Jankevics

The present invention relates to a method for testing a substance interacting with a target molecule in a cell by fluorescence correlation spectroscopy (FCS) by adding a test substance to the cell and determining a distribution of diffusion coefficients fo ...

2006

Determining a receptor-ligand interaction useful in e.g. drug screening involves repetitive application of reversibly binding fluorescent ligands with fast association-dissociation kinetics

Horst Arthur Vogel, Rudolf Hovius, Jean-Manuel Segura, Marinela Gjoni Boillat, Christoph Schreiter

NOVELTY - Determining a receptor-ligand interaction involves repeatedly contacting a cell comprising a receptor, with a ligand, of the receptor in which the ligand has a fluorescence labeling group and binds reversibly to the receptor under conditions in w ...

2006

Diffusion-Time Distribution Analysis Reveals Characteristic Ligand-Dependent Interaction Patterns of Nuclear Receptors in Living Cells

Horst Arthur Vogel, Horst Pick, Hanna Jankevics, Paulina Izewska

Nuclear receptors initiate transcription, interact with regulatory proteins, and are influenced by hormones, drugs, and pollutants. Herein, we discover ligand-specific mobility patterns of human estrogen receptor-a (ER) in living cells using diffusion-time ...

2005

Reversible sequential-binding probe receptor-ligand interactions in single cells

Horst Arthur Vogel, Rudolf Hovius, Jean-Manuel Segura, Marinela Gjoni Boillat, Christoph Schreiter

With the reversible sequential (ReSeq) binding assay, we present a novel approach for the ultrasensitive profiling of receptor function in single living cells. This assay is based on the repetitive application of fluorescent ligands that have fast assocn.- ...

2005